FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005028861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35995930
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAC22350.027.4808031
GCGACCA32900.020.2763143
GGCGACC34550.019.5182022
CGACCAC40400.016.6909524
ACCGAGA61650.011.2886419
CCACCGA64300.010.7106587
ACGACGC14250.09.86949945-49
CGACGCT15500.09.72836645-49
TATGCCG60250.09.12053745-49
CACCGAG80300.08.57652958
ACCACCG82750.08.4978156
TCTCGTA70150.07.83341940-44
CTCGTAT71100.07.810322840-44
CGTATGC69050.07.79019845-49
CACGACG19600.06.8796345-49
GATCGGA192200.06.84773251
ATGCCGT80900.06.774566745-49
GCCGTCT82900.06.681156650-54
TATCTCG77900.06.64462640-44
ATCGGAA210400.06.5825252
Produced by FastQC (version 0.11.5)