FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005028941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22671800
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGTGAATCTCGTAT251540.11094840286170483TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA139500.029.1766451
ATCGGAA145900.027.980372
TCGGAAG150650.026.9019413
CGGAAGA160500.025.3393944
TATGCCG52750.016.43679445-49
AGAGCAC278950.014.8654498
CGTATGC59200.014.6459645-49
TCTCGTA59150.014.29065840-44
CTCGTAT59350.014.02263340-44
GAGCACA302300.013.8611139
GAAGAGC316150.013.3456026
ATGCCGT67200.012.47087645-49
GCCGTCT71900.012.341350-54
TGCCGTC69500.011.80782750-54
TCGTATG69550.011.77849145-49
ATCTCGT73750.011.5992140-44
CCGTCTT85550.010.45691450-54
CGTCTTC85100.010.39294650-54
AAGAGCA439650.09.9595177
GGAAGAG508750.08.5212865
Produced by FastQC (version 0.11.5)