FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005028966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005028966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65307350
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC288100.014.1432289
AGAGCGT330450.012.39588
GATCGGA337850.012.34971
CGCCGTA80150.011.63192855-59
ATCGGAA361350.011.5612882
TCGGAAG374900.011.2352373
CGGAAGA401000.010.5580094
AAGAGCG406250.010.3148047
TCGCCGT96200.09.8570555-59
GTCGCCG111050.08.56504355-59
GCCGTAT115300.08.34993555-59
TGGTCGC134950.07.22000550-54
GTGGTCG145650.06.80905350-54
CGTATCA147950.06.67383960-64
GGTCGCC140050.06.22208155-59
CCGTATC138500.06.17654860-64
GAAGAGC872550.05.5585616
TCTCGGT202050.05.159569745-49
GCGTCGT208000.05.022478145
Produced by FastQC (version 0.11.5)