FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005029127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005029127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71529190
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG252250.08.03539945-49
TCGGAAG1312250.07.076643
ATCGGAA1322450.07.063562
CGGAAGA1319900.07.03559884
GATCGGA1338250.06.9162781
ATGCCGT292100.06.914345345-49
GCCGTCT292750.06.8047850-54
CGTATGC292800.06.73440745-49
TCTCGTA292250.06.712392340-44
CTCGTAT294450.06.672088640-44
TGCCGTC291450.06.56152850-54
CGTCTTC329200.06.337605550-54
AGAGCAC1525900.06.23328
GAGCACA1545800.06.15764679
CCGTCTT342950.06.01586950-54
TCGTATG319650.05.99636845-49
AGATCGG1350950.05.8973017145
GAAGAGC1680350.05.78093436
ATCTCGT345900.05.696432640-44
TAGATCG420200.05.663802145