FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005029131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005029131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31769213
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA156950.031.2005441
ATCGGAA170350.028.6247352
TCGGAAG177850.027.2459133
CGGAAGA189250.025.527854
TATGCCG76450.016.1822345-49
CTCGTAT86150.014.56216440-44
TCTCGTA84250.013.996448540-44
AGAGCAC377150.013.1745468
CGTATGC85000.013.02064245-49
ATGCCGT99150.012.60886845-49
GAGCACA405200.012.3161649
GAAGAGC421450.011.8584356
ATCTCGT102650.011.23356140-44
GCCGTCT99700.011.04293150-54
CGTCTTC117650.010.7125650-54
TCGTATG93850.010.63513445-49
TGCCGTC98350.010.1045250-54
CCGTCTT120100.010.09597850-54
AAGAGCA586550.08.8663267
ACACGTC143200.08.45703110-14
Produced by FastQC (version 0.11.5)