FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005029142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005029142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104998874
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTGAAACGTGTAGATCT2217700.21121178880451613Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC816050.042.9059079
AGAGCGT883100.039.7632188
GATCGGA923050.038.9372021
ATCGGAA977700.036.8285682
TCGGAAG999850.035.960693
AAGAGCG1005350.035.2812047
CGGAAGA1038300.034.6430134
CGCCGTA347350.022.39755855-59
TCGCCGT368950.020.90953655-59
GAAGAGC1757450.020.6855896
GTCGCCG401900.019.70011155-59
GCCGTAT401850.019.36715355-59
TGGTCGC435300.018.10934850-54
CGTATCA444450.017.60565860-64
GTGGTCG457200.017.4257750-54
CCGTATC429900.016.0270560-64
GGTCGCC449400.015.47006555-59
TCTCGGT549250.014.94648145-49
GGAAGAG2664700.014.00443655
CGGTGGT576600.013.963150-54
Produced by FastQC (version 0.11.5)