FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005029191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005029191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50484275
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC31550.013.60281225-29
CGACGCT32200.013.5533625-29
GATCGGA518600.012.5970671
TCGGAAG534200.012.4198543
ATCGGAA531000.012.4130132
TATGCCG142000.012.07904645-49
CGGAAGA549300.012.0521094
CACGACG37450.011.8081820-24
GTTGGAC213900.011.3903312
CGTATGC162750.010.34302245-49
CTCGTAT163900.010.28827640-44
TCTCGTA161800.010.28739140-44
ATGCCGT171950.09.75590245-49
GCCGTCT177450.09.48618150-54
TATCTCG170850.09.36905940-44
TCCGATC52050.08.774857535-39
TGGACTA287250.08.5826374
CGTCTTC207100.08.54112250-54
AGAGCAC789800.08.5199918
ATCTCGT192800.08.48289140-44
Produced by FastQC (version 0.11.5)