FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005029199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005029199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19916606
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAC27600.058.8466151
GCGACCA33900.054.7502023
CGACCAC36950.050.031554
GGCGACC39050.047.3520472
ACCGAGA51550.035.4396139
CCACCGA55150.034.7036827
ACCACCG65050.029.5335546
CACCGAG63100.029.5271058
ACGACGC20350.017.81241245-49
GACCACC121450.016.2363955
CGACGCT23300.015.99279345-49
CACGACG23700.014.9887345-49
TCCGATC27050.013.668509555-59
CCGATCT34450.011.27960155-59
TACACGA33500.011.12363640-44
ACACGAC37650.09.82050140-44
TTCCGAT36850.09.67934255-59
CTACACG41300.09.61964340-44
GACGCTC40600.09.28526845-49
ACGCTCT45000.08.50625845-49
Produced by FastQC (version 0.11.5)