FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005029201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005029201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59378693
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAC84250.069.0359041
GCGACCA98000.063.8397523
CGACCAC112750.056.0668834
GGCGACC112400.055.27552
ACCGAGA152000.041.4463169
CCACCGA163100.039.1142357
CACCGAG192600.033.1987768
ACCACCG194500.032.72516
ACGACGC68250.017.9746345-49
GACCACC366400.017.9060255
CGACGCT70100.017.54163745-49
CACGACG76400.015.60165745-49
TCCGATC84850.014.64497355-59
TACACGA107300.012.13487740-44
CCGATCT108300.011.84880155-59
ACACGAC110250.011.49454140-44
TTCCGAT110100.011.14147455-59
GACGCTC120400.010.61064345-49
CTACACG125650.010.24729540-44
ACGCTCT138700.09.46159645-49
Produced by FastQC (version 0.11.5)