FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005029215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005029215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22690486
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAGCTGATCTCGTAT419670.18495416977847015TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA146300.050.8448521
ATCGGAA156300.047.9470862
TCGGAAG159800.046.709953
CGGAAGA171800.043.4051444
AGAGCAC294350.025.5031018
GAGCACA313500.023.5752379
GAAGAGC327550.023.0288056
TATGCCG78350.020.39260745-49
CTCGTAT83700.018.59285240-44
TCTCGTA81350.018.237740-44
CGTATGC87600.017.7219745-49
AAGAGCA449000.017.1063297
ATGCCGT95050.016.45596145-49
TCGTATG93150.016.02265545-49
GCCGTCT95350.016.01801750-54
TGCCGTC93550.015.5192250-54
ATCTCGT93750.015.4383440-44
GGAAGAG504500.015.3680915
CGTCTTC109250.014.43185250-54
CCGTCTT111350.013.78156350-54
Produced by FastQC (version 0.11.5)