FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23506074
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258380.10992052522254461No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCAA929400.0139.143519
TGGTCAA842450.0137.858989
CGGTCAA805600.0137.748259
TCGTCAA684500.0137.555889
GGGTCAA1053100.0137.050319
GAGTCAA549650.0136.560099
GGTTCCG628550.0136.224089
AGGTCAA608800.0135.212889
GTGTCAA931950.0135.090879
CAGTCAA530100.0134.634899
CCCGTCA140750.0134.318548
ATGTCAA752650.0134.29039
CATTCCG242800.0134.222349
TGTTCCG434850.0134.064689
GCGTCAA714400.0134.061849
CTTTCCG282850.0133.928799
CGTTCCG355200.0133.632839
AGTTCCG355000.0133.115869
CCGGTCA188250.0132.48938
GCTTCCG375900.0131.404399
Produced by FastQC (version 0.11.5)