FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24824921
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG291880.11757539933359706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCAA791050.0137.541619
CGGTCAA693150.0137.146859
GGTTCCG683750.0136.455679
TGGTCAA753250.0136.454979
GAGTCAA499750.0135.514189
GGGTCAA935900.0135.171559
TGTTCCG469550.0134.73419
TCGTCAA622500.0134.450099
CATTCCG268550.0134.338159
CGTTCCG383950.0134.238789
GCTTCCG403950.0133.874289
GTGTCAA817400.0133.675459
AGGTCAA553050.0133.64949
AGTTCCG391450.0133.092969
CAGTCAA477900.0133.062689
GCGTCAA650050.0132.956659
CTTTCCG299850.0132.91259
ATGTCAA646450.0132.171789
CCGGTCA165550.0131.816368
TTAGTAA544000.0130.47529
Produced by FastQC (version 0.11.5)