FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11008959
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGAA31700.0125.557029
CGTCTAC47000.0124.943911
TGACTAG26250.0124.282831
CACCGAA26600.0117.466418
CTCTACG57300.0116.275661
CATCGAA39300.0115.29631
TTGGACG19100.0115.010422
ATTGGAC57950.0114.846341
CGCTTCA45850.0114.4793551
CGCGGTA64200.0114.280831
AGTACGA28950.0114.191648
CGTACGA21150.0113.800927
TCGCAAG30200.0113.786168
CGCTATC33650.0113.7566451
TACACGA45600.0113.676081
CTAATGG30100.0112.7216341
ACACGAT30700.0112.4078142
TAGCCGG43900.0112.298071
GGCTCGA35650.0112.2554551
AGGACGA35700.0111.28348
Produced by FastQC (version 0.11.5)