FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11008959
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC5477674.975647561227179No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC4049173.6780680171485787No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC2803802.5468348097217914No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC2006381.8224974768277362No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA591450.5372442571545593No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT288700.2622409621109498No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC243800.2214559977923435No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG231720.21048311652355142No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC150580.13677950839856884No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG146070.13268284494474No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT142600.1295308666332575No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT141380.1284226782931974No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGAAGAG140520.12764149634856484No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG139420.1266423101403139No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA135410.12299982223569005No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG132980.12079252906655388No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC127920.1161962725085996No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGG124070.11269912077972132No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC122480.1112548425332495No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT121360.11023748930303037No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC115560.10496905293225273No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG114240.1037700294823516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG1052000.0131.807461
AGTCTCT789250.0113.2241741
GTCTCTC809100.0109.944622
TCAGCTG868000.0102.521697
TCTCTCA874850.0101.6646043
AGCTGGT886500.0100.196379
CAGCTGG896500.098.488358
CTCAGCT958350.092.0880056
CTCTCAG998400.089.054364
TCTCAGC1029350.086.0668265
GGCTCAA1810350.075.859967
CTCAAAC1824300.075.583649
GCTCAAA1829100.075.2386258
CTGATGG1855800.074.444422
GATGGCT1850200.074.104814
ATGGCTC1891300.072.103445
TGGCTCA1969850.069.5855566
TGATGGC2063400.066.869893
TCAAACA1851600.055.4427349
CGCAAAC18150.047.1372839
Produced by FastQC (version 0.11.5)