FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10601228
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA47850.0131.207051
CGTCGAA14050.0129.513347
CGGCTAG51700.0127.18561
CCCGATA22200.0125.7271042
GATTGAC39450.0124.595942
CGTTGGG57200.0123.9549941
CGAACGG29200.0122.152819
CCGGTAG44250.0122.057431
CGCTATC28750.0117.760661
GGCTCGA36800.0116.034911
CCGAACG20100.0115.057739
CGAAGTC26150.0114.775134
GATTACG13100.0114.003222
GATCGCG35250.0113.527511
TTTCGAA26700.0113.496956
ATTGACA43500.0112.995633
ATCGTAC14850.0112.284167
TTACCGA20100.0112.172279
CTACACG26650.0112.07822
GACAATA30600.0111.8257459
Produced by FastQC (version 0.11.5)