FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10601228
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC5911385.576127595784187No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC4775664.504817743755724No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2167372.04445183142934No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1811831.7090755901108816No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT340000.3207175621541203No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC296180.2793827281141392No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG223300.21063597537945605No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA184100.173659127036981No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG179070.16891439369099503No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC176140.16615056293478453No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT159620.15056746256188436No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGAGAG134480.12685322870142968No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT133570.12599483757919366No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG115210.10867608922287117No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC114170.10769507079745856No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTT106320.10029026825948842No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG1036700.0131.95661
AGTCTCT550500.0114.221371
GTCTCTC578000.0108.573732
TCTCTCA632300.099.146553
TCAGCTG645950.097.321267
AGCTGGT705700.088.755049
CAGCTGG740950.084.1314248
CTCTCAG745700.083.874714
CTCAGCT794500.078.539686
GGCTCAA1879400.072.1912847
CTCAAAC1896450.071.754549
GCTCAAA1903000.071.423758
CTGATGG1922350.071.015512
TCTCAGC889550.070.3927465
ATGGCTC1919300.070.082025
GATGGCT1945900.069.567384
TGGCTCA2018300.067.08926
TGATGGC2046400.066.565393
TCAAACA1922900.058.8378529
CGCAAAC18050.047.799079
Produced by FastQC (version 0.11.5)