FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9898138
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTA47100.0121.4431468
TGCGGAA37000.0120.699892
CGTAACA23050.0118.261893
GAGCGAA29000.0116.7472842
TGACTAG41350.0116.418081
TATTACC46450.0116.278241
CGATTGA30950.0115.950451
AATCCGA17900.0115.834129
AGACGAC23850.0115.204058
GCGGAAG38850.0114.765663
GCGCGAT33950.0113.819151
CATATTC44500.0112.7389451
ACTAGAG29200.0112.719983
GCGCCAA35850.0112.235881
CTCGTAC46800.0112.0005951
CTGCGGA45850.0111.94941
TATCGCA16400.0111.8407759
TGCGTGG37600.0111.83251
CGAGCAC43850.0111.599461
CTGATAG69200.0111.471381
Produced by FastQC (version 0.11.5)