FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9898138
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC5435805.491739961596818No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC4170734.213651092761083No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTTGGGTC2882042.911699149880513No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1838021.8569351124423605No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1466181.4812684971658305No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTTGGGTCT515230.5205322455597204No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTTGGGTCTTC299550.30263267697419455No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT291790.29479281860891415No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG288050.2910143301699774No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTTGGAAA265860.26859597229297066No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC248320.2508754676889734No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATC175770.17757885371976023No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT140490.14193578630647502No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG139910.1413498175111319No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA135330.1367226846099741No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG134930.13631856819939264No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA133950.13532848299346806No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT123090.12435672244618129No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC115840.11703211250439224No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA113280.11444576747667086No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT103410.10447419504557322No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG964400.0127.393061
AGTCTCT996500.0120.5901951
GTCTCTC1039150.0115.1942752
TCTCTCA1098200.0109.1581953
TCAGCTG1143650.0104.694397
CAGCTGG1179000.0101.158698
AGCTGGT1185950.0100.926579
CTCTCAG1241050.096.381874
CTCAGCT1247350.095.516316
TCTCAGC1280950.093.232545
GGCTCAA1639600.074.220087
CTCAAAC1653000.073.8794259
GCTCAAA1661950.073.359428
CTGATGG1682850.072.88092
GATGGCT1697550.071.590624
TGATGGC1822150.067.141843
ATGGCTC1802350.067.06995
GGTGTTC593050.065.940544145
TGGCTCA1880500.064.578816
TCAAACA1682500.057.1183669
Produced by FastQC (version 0.11.5)