FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5720388
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC3172965.54675661860699No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3132525.47606211326924No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC866491.514739909250911No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC860831.5048454755166958No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT250840.43850172400893084No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA229490.40117908085955006No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC205970.3600629887343306No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT200300.3501510736684295No Hit
GAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCAGA128100.22393585889628467No Hit
CCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGAC124770.21811457544488239No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTAAGATCC112950.19745164139215732No Hit
GCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGA112430.1965426121444909No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA111320.19460218432735682No Hit
GTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCACACAGCAGAGGAGCC111200.1943924083471261No Hit
GCTAAACATTATCTTCAGAAATTTCTTAGAGTCCTGTTTATACCAATACA107930.1886760128858392No Hit
GCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCACACAGCAGAG107240.18746980099951263No Hit
GTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTT105610.18462034393471213No Hit
CCAATACATAGTATCATGGCCCAGATTTTGTTCACATTTAATGGACTTGT101380.17722574063157953No Hit
GTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGT100270.17528531281444545No Hit
GGCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTG96800.16921929071944072No Hit
GTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGAC93460.16338052593635258No Hit
GTCCTGTTTATACCAATACATAGTATCATGGCCCAGATTTTGTTCACATT86920.15194773501377878No Hit
CTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCA84730.1481193233745683No Hit
GGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGG82690.1445531317106462No Hit
GGCAGAAGACCACACAGCAGAGGAGCCTGCAGCCCATGGCAGAGTGAGGC79420.1388367362493593No Hit
GCTCCTTATTATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCC79220.13848710961564145No Hit
GTTTCATTTATAATGAGCTCCTTATTATTGTAGCTAAACATTATCTTCAG77740.13589987252612934No Hit
GGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAA76770.13420418335259776No Hit
CCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAA75660.13226375553546368No Hit
CTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAG74970.13105754364913708No Hit
GGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCACAC74860.13086524900059227No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG73420.12834793723782373No Hit
GGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCACA72830.12731653866835604No Hit
GTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTG72180.12618025210877304No Hit
CACATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGA68960.1205512633059156No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG68680.12006178601871062No Hit
CCTGTTTATACCAATACATAGTATCATGGCCCAGATTTTGTTCACATTTA68170.11917023810273009No Hit
GTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCCTGTTTATACCAAT66370.11602359839926941No Hit
ATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGG66100.11555160244375032No Hit
AGAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCAG62470.1092058790417713No Hit
CAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCACACAGCAG62390.10906602838828415No Hit
CGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTG62380.10904854705659826No Hit
GACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTT61880.10817448047230362No Hit
ATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGA61020.10667108594731686No Hit
GTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGC60970.1065836792888874No Hit
CATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGT60860.10639138464034258No Hit
GTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCT60780.10625153398685543No Hit
CAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGA60750.10619908999179777No Hit
GGAGGCAGAAGACCACACAGCAGAGGAGCCTGCAGCCCATGGCAGAGTGA60430.10563968737784919No Hit
CATAGTATCATGGCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTC59670.10431110616972135No Hit
CATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGG59600.10418873684792011No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58740.10268534232293335No Hit
ATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGT58060.10149661176829262No Hit
TGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCT57860.10114698513457479No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT57830.1010945411395171No Hit
CAGAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCA57770.10098965314940175No Hit
GTATCATGGCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCAT57500.10051765719388266No Hit
GTTTATACCAATACATAGTATCATGGCCCAGATTTTGTTCACATTTAATG57430.1003952878720814No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)