FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11585849
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTC5359124.625573835806077No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC4806444.148543624209154No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC4208553.6324916715210076No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3810093.2885721193155546No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC3196702.7591417771800755No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTC1546401.3347317058939747No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCA982500.8480172665809816No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCACA591620.5106401783762243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG317220.273799529063429No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC310850.2683014425615248No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTCA287520.24816480863853826No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC285550.24646445849587717No Hit
GAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCAGA281180.24269261579362894No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT274500.23692696150277806No Hit
CCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGAC259460.2239456081293654No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT251000.21664359685682077No Hit
GCTAAACATTATCTTCAGAAATTTCTTAGAGTCCTGTTTATACCAATACA235770.2034982503224408No Hit
GCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGA222930.1924157651286496No Hit
GTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTT222280.19185473589376142No Hit
CCAATACATAGTATCATGGCCCAGATTTTGTTCACATTTAATGGACTTGT216830.18715072153969897No Hit
GTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCACACAGCAGAGGAGCC206460.17820014743848292No Hit
GGCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTG202830.1750670149421074No Hit
GCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCACACAGCAGAG200260.17284879165954953No Hit
GTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGT199810.17246038680462691No Hit
GTCCTGTTTATACCAATACATAGTATCATGGCCCAGATTTTGTTCACATT194300.1677045851365748No Hit
GCTCCTTATTATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCC182540.15755427159459787No Hit
GTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGAC181430.15659620628578882No Hit
GTTTCATTTATAATGAGCTCCTTATTATTGTAGCTAAACATTATCTTCAG178730.15426577715625328No Hit
GTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCT177420.15313508746747864No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTCACC173760.1499760613141083No Hit
CTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCA167340.1444348187172127No Hit
GGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGG161210.13914388147126724No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA160710.13871232052135324No Hit
CTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAG156900.13542382608300868No Hit
GTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTG153140.13217848773965551No Hit
GTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCCTGTTTATACCAAT152290.13144483412480173No Hit
CCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAA151520.1307802302619342No Hit
CCTGTTTATACCAATACATAGTATCATGGCCCAGATTTTGTTCACATTTA149660.12917482352825416No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG146840.12674081977073928No Hit
CACATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGA144280.12453122770717968No Hit
GGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAA143700.12403061700527945No Hit
GGCAGAAGACCACACAGCAGAGGAGCCTGCAGCCCATGGCAGAGTGAGGC143540.12389251750130699No Hit
GCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCACATT141680.12228711076762695No Hit
GGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAGCCACAG140700.12144125130579556No Hit
CCAAGTTCTTGCTTATACCAAGGGAAGTAGTTTGAGGCACTGTCTGAATA139550.12044866112099338No Hit
GGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCACA136450.11777298323152666No Hit
GGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCACAC135410.11687533645570558No Hit
ATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGG134130.11577054042392577No Hit
GTTGAAGGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAG133840.11552023507297565No Hit
AGAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCAG133750.11544255410199115No Hit
GGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGA131870.11381988493031456No Hit
CAGAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCA131020.11308623131546078No Hit
GTTTATACCAATACATAGTATCATGGCCCAGATTTTGTTCACATTTAATG130030.11223174063463108No Hit
GGAGATTTAGGTGAGAAGCGATTTGGAACTGTTTCATTTATAATGAGCTC129780.1120159601596741No Hit
GTGAGCTTTGTCTGGAGATTTAGGTGAGAAGCGATTTGGAACTGTTTCAT129430.11171386749473432No Hit
GAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGT129320.11161892408575323No Hit
GTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCA127650.11017751051304052No Hit
AGTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAG127010.10962511249715061No Hit
CCTTATTATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCCTGT125960.10871883450233125No Hit
CTTCAGAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGC125050.10793339357348779No Hit
GCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAGCCACAGGAATATA125030.10791613113549123No Hit
ATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGA124600.1075449887185652No Hit
CGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTG124540.10749320140457552No Hit
CAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGA123370.1064833487817768No Hit
ATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGT123210.10634524927780432No Hit
ATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCCTGTTTATACC123000.10616399367884045No Hit
GGTCTTTTTCCAAGTTCTTGCTTATACCAAGGGAAGTAGTTTGAGGCACT122200.10547349615897808No Hit
CAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCACA121700.10504193520906409No Hit
GACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTT120830.10429101915621376No Hit
GGTGAGAAGCGATTTGGAACTGTTTCATTTATAATGAGCTCCTTATTATT120490.10399755771027225No Hit
GTATCATGGCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCAT120060.10362641529334622No Hit
GTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGC119660.10328116653341501No Hit
GTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCT118070.10190880271268857No Hit
ATGAGCTCCTTATTATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGA117840.10171028467572812No Hit
ATCATGGCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCT117600.10150313541976942No Hit
CAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCACACAGCAG117570.10147724176277458No Hit
CATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGT117520.10143408566778318No Hit
AATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGG116750.10076948180491564No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)