FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13025277
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAAA59300.0131.668148
ATGCGAT69150.0125.809591
GACCAAT73500.0124.972361
TACACGA39050.0123.274071
CAGACGC60250.0123.214738
TTACGCA22850.0123.1034553
ACAGCCG98900.0123.004311
TCGCGAG39350.0122.3342361
TTCGCGA29200.0121.906919
CTCGAAA57700.0121.625391
TAAACCG41300.0121.4732741
TCCACGA56900.0121.040598
GTTTGAC53050.0120.532148
TCGTCCG65450.0119.406748
CTAACGG23800.0119.104869
ATCGTCC59150.0118.642517
CTACGTC50750.0118.5677951
CGACGGA44050.0118.170279
TCGCGAA22150.0118.1580059
ATAGACC62900.0117.6792141
Produced by FastQC (version 0.11.5)