FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13025277
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC7320015.619849773636292No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC5183663.979692715939937No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA4288863.292720761331985No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAA775910.5956955848232632No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGAGAG598620.4595833163471303No Hit
GTCAGGCTCTGTCCAGGTTGCTGACGGTACCAGAACATCATGGTGACTTG479700.36828391442270286No Hit
GTCCAGGTTGCTGACGGTACCAGAACATCATGGTGACTTGGCTATCGACT456240.35027278114699595No Hit
CTGACGGTACCAGAACATCATGGTGACTTGGCTATCGACTTGACACTGGA437540.3359160807098383No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACT434360.33347467389753016No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA416970.3201237102289648No Hit
CTTGGCTATCGACTTGACACTGGATCGTCAGGGAGGTTCCACGTTGACAG409150.31411999913706246No Hit
GTCAGGGAGGTTCCACGTTGACAGATATCCCTGCTTGGCTTTTGAGAGAT396430.3043543718878301No Hit
GTTGCGATCAGTGTCAGGCTCTGTCCAGGTTGCTGACGGTACCAGAACAT392290.3011759366038818No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT379740.29154082481316906No Hit
GCCTCAGAGCCCTGATTTGCAGTTGCGATCAGTGTCAGGCTCTGTCCAGG370220.2842319591360706No Hit
GCCCTGATTTGCAGTTGCGATCAGTGTCAGGCTCTGTCCAGGTTGCTGAC369770.2838864770399892No Hit
CCAGAACATCATGGTGACTTGGCTATCGACTTGACACTGGATCGTCAGGG350430.26903842428840474No Hit
GCTGACGGTACCAGAACATCATGGTGACTTGGCTATCGACTTGACACTGG346720.266190116340712No Hit
GGTTGCTGACGGTACCAGAACATCATGGTGACTTGGCTATCGACTTGACA343800.2639483214061398No Hit
GACTTGACACTGGATCGTCAGGGAGGTTCCACGTTGACAGATATCCCTGC343780.26393296664631394No Hit
GGCTCTGTCCAGGTTGCTGACGGTACCAGAACATCATGGTGACTTGGCTA338730.26005588979029004No Hit
GACGGTACCAGAACATCATGGTGACTTGGCTATCGACTTGACACTGGATC336420.2582824150304059No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC334220.2565933914495638No Hit
GGAAACTTGTCAATGACAAATCCACTCTCATATGTGGCCTCAGAGCCCTG329010.25259347651493325No Hit
GTGTCAGGCTCTGTCCAGGTTGCTGACGGTACCAGAACATCATGGTGACT328470.2521788979996356No Hit
GCTCTGTCCAGGTTGCTGACGGTACCAGAACATCATGGTGACTTGGCTAT309660.23773774638343584No Hit
GATCAGTGTCAGGCTCTGTCCAGGTTGCTGACGGTACCAGAACATCATGG308850.2371158786104894No Hit
CCTCAGAGCCCTGATTTGCAGTTGCGATCAGTGTCAGGCTCTGTCCAGGT305230.2343366670820129No Hit
GTTCCACGTTGACAGATATCCCTGCTTGGCTTTTGAGAGATGACAGCACT290680.22316607930871643No Hit
GGTTTGGGCGGCTGATGGGAAACTTGTCAATGACAAATCCACTCTCATAT279450.21454438166650888No Hit
CTATCGACTTGACACTGGATCGTCAGGGAGGTTCCACGTTGACAGATATC278360.21370754725600077No Hit
GGCGGCTGATGGGAAACTTGTCAATGACAAATCCACTCTCATATGTGGCC275120.21122007616421515No Hit
AGAACATCATGGTGACTTGGCTATCGACTTGACACTGGATCGTCAGGGAG271360.20833338131695778No Hit
CGATCAGTGTCAGGCTCTGTCCAGGTTGCTGACGGTACCAGAACATCATG270660.2077959647230535No Hit
CCAGGTTGCTGACGGTACCAGAACATCATGGTGACTTGGCTATCGACTTG269520.20692074341298075No Hit
GTCAGAGTTGAGAATGTTAGGTTTGGGCGGCTGATGGGAAACTTGTCAAT262470.2015081905743732No Hit
GGGAGGTTCCACGTTGACAGATATCCCTGCTTGGCTTTTGAGAGATGACA252660.19397668087980008No Hit
GTGGCCTCAGAGCCCTGATTTGCAGTTGCGATCAGTGTCAGGCTCTGTCC252560.1938999070806709No Hit
ATGGTGACTTGGCTATCGACTTGACACTGGATCGTCAGGGAGGTTCCACG251940.19342390952606997No Hit
GGCCTCAGAGCCCTGATTTGCAGTTGCGATCAGTGTCAGGCTCTGTCCAG246260.18906315773553223No Hit
CTGATTTGCAGTTGCGATCAGTGTCAGGCTCTGTCCAGGTTGCTGACGGT242670.18630697834679447No Hit
GGATCGTCAGGGAGGTTCCACGTTGACAGATATCCCTGCTTGGCTTTTGA238890.18340492873971126No Hit
GTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCT236810.1818080337178242No Hit
GGTGACTTGGCTATCGACTTGACACTGGATCGTCAGGGAGGTTCCACGTT236640.1816775182593046No Hit
CGGTACCAGAACATCATGGTGACTTGGCTATCGACTTGACACTGGATCGT234920.18035700891428258No Hit
GTACCAGAACATCATGGTGACTTGGCTATCGACTTGACACTGGATCGTCA234170.17978120542081372No Hit
GCAGTTGCGATCAGTGTCAGGCTCTGTCCAGGTTGCTGACGGTACCAGAA232480.17848372821553046No Hit
GAAACTTGTCAATGACAAATCCACTCTCATATGTGGCCTCAGAGCCCTGA229940.17653367371764914No Hit
GGGAAACTTGTCAATGACAAATCCACTCTCATATGTGGCCTCAGAGCCCT227210.1744377490014224No Hit
GCTGATGGGAAACTTGTCAATGACAAATCCACTCTCATATGTGGCCTCAG225290.17296369205814202No Hit
GTTTGGGCGGCTGATGGGAAACTTGTCAATGACAAATCCACTCTCATATG219690.16866435930690762No Hit
CCCTGATTTGCAGTTGCGATCAGTGTCAGGCTCTGTCCAGGTTGCTGACG216410.16614617869547035No Hit
AATCCACTCTCATATGTGGCCTCAGAGCCCTGATTTGCAGTTGCGATCAG215510.1654552145033077No Hit
CTCATATGTGGCCTCAGAGCCCTGATTTGCAGTTGCGATCAGTGTCAGGC214320.16454160629367037No Hit
ACCAGAACATCATGGTGACTTGGCTATCGACTTGACACTGGATCGTCAGG212450.1631059362499546No Hit
GTTGAGAATGTTAGGTTTGGGCGGCTGATGGGAAACTTGTCAATGACAAA211470.16235355301848858No Hit
CAATGACAAATCCACTCTCATATGTGGCCTCAGAGCCCTGATTTGCAGTT209750.1610330436734666No Hit
CAGGTTGCTGACGGTACCAGAACATCATGGTGACTTGGCTATCGACTTGA206930.15886802253802357No Hit
CAAATCCACTCTCATATGTGGCCTCAGAGCCCTGATTTGCAGTTGCGATC205850.15803886550742838No Hit
GATGGGAAACTTGTCAATGACAAATCCACTCTCATATGTGGCCTCAGAGC201880.1549909456819997No Hit
GTTAGGTTTGGGCGGCTGATGGGAAACTTGTCAATGACAAATCCACTCTC201470.15467617310557003No Hit
CACTGGATCGTCAGGGAGGTTCCACGTTGACAGATATCCCTGCTTGGCTT200220.15371650061645523No Hit
GACAGATATCCCTGCTTGGCTTTTGAGAGATGACAGCACTGAACACAGAG200150.1536627589570648No Hit
GCGGCTGATGGGAAACTTGTCAATGACAAATCCACTCTCATATGTGGCCT199300.1530101816644667No Hit
ATCATGGTGACTTGGCTATCGACTTGACACTGGATCGTCAGGGAGGTTCC196790.15108315930632416No Hit
CAGGCTCTGTCCAGGTTGCTGACGGTACCAGAACATCATGGTGACTTGGC196740.15104477240675956No Hit
GTTGCTGACGGTACCAGAACATCATGGTGACTTGGCTATCGACTTGACAC189370.1453865434109386No Hit
GACTTGGCTATCGACTTGACACTGGATCGTCAGGGAGGTTCCACGTTGAC188670.1448491268170343No Hit
CTTGTCAATGACAAATCCACTCTCATATGTGGCCTCAGAGCCCTGATTTG188320.14458041852008213No Hit
GCGATCAGTGTCAGGCTCTGTCCAGGTTGCTGACGGTACCAGAACATCAT187990.14432706498295583No Hit
GTGACTTGGCTATCGACTTGACACTGGATCGTCAGGGAGGTTCCACGTTG185420.14235397834533578No Hit
AATGACAAATCCACTCTCATATGTGGCCTCAGAGCCCTGATTTGCAGTTG185410.14234630096542283No Hit
GACAAATCCACTCTCATATGTGGCCTCAGAGCCCTGATTTGCAGTTGCGA185310.14226952716629365No Hit
GCTTTTGAGAGATGACAGCACTGAACACAGAGCCTAGTCCCAGGAGAAGG184090.1413328868169176No Hit
CAGGGAGGTTCCACGTTGACAGATATCCCTGCTTGGCTTTTGAGAGATGA181670.1394749608779913No Hit
GATATCCCTGCTTGGCTTTTGAGAGATGACAGCACTGAACACAGAGCCTA179650.13792413013558177No Hit
ACTTGACACTGGATCGTCAGGGAGGTTCCACGTTGACAGATATCCCTGCT178690.13718710166394157No Hit
CAGAACATCATGGTGACTTGGCTATCGACTTGACACTGGATCGTCAGGGA178590.1371103278648124No Hit
GCTCACAGTCAGAGTTGAGAATGTTAGGTTTGGGCGGCTGATGGGAAACT177920.13659594341064685No Hit
GCTATCGACTTGACACTGGATCGTCAGGGAGGTTCCACGTTGACAGATAT177890.1365729112709081No Hit
AGGCTCTGTCCAGGTTGCTGACGGTACCAGAACATCATGGTGACTTGGCT177560.1363195577337818No Hit
CCAAGTTCTTGCTTATACCAAGGGAAGTAGTTTGAGGCACTGTCTGAATA176120.13521401502632152No Hit
ATCAGTGTCAGGCTCTGTCCAGGTTGCTGACGGTACCAGAACATCATGGT175760.13493762934945644No Hit
GGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGA174910.13428505205685837No Hit
GTCAATGACAAATCCACTCTCATATGTGGCCTCAGAGCCCTGATTTGCAG173870.13348660454591485No Hit
GGTTCCACGTTGACAGATATCCCTGCTTGGCTTTTGAGAGATGACAGCAC173760.13340215336687275No Hit
CTGATGGGAAACTTGTCAATGACAAATCCACTCTCATATGTGGCCTCAGA172260.13225054637993494No Hit
GGTACCAGAACATCATGGTGACTTGGCTATCGACTTGACACTGGATCGTC171700.13182061310481152No Hit
CCTGATTTGCAGTTGCGATCAGTGTCAGGCTCTGTCCAGGTTGCTGACGG170810.13113732629256178No Hit
ATCGACTTGACACTGGATCGTCAGGGAGGTTCCACGTTGACAGATATCCC170770.13110661677291008No Hit
CTTTTGAGAGATGACAGCACTGAACACAGAGCCTAGTCCCAGGAGAAGGA167240.12839650166364983No Hit
CAGTTGCGATCAGTGTCAGGCTCTGTCCAGGTTGCTGACGGTACCAGAAC165400.12698386375967283No Hit
GCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCACATT164030.12593206271160298No Hit
CTCAGAGCCCTGATTTGCAGTTGCGATCAGTGTCAGGCTCTGTCCAGGTT162220.12454245694736472No Hit
GCTGTCTTCAGGGCTCATGTTGCTCACAGTCAGAGTTGAGAATGTTAGGT161600.12406645939276378No Hit
GGGCTCATGTTGCTCACAGTCAGAGTTGAGAATGTTAGGTTTGGGCGGCT161310.12384381537528914No Hit
CTCTCATATGTGGCCTCAGAGCCCTGATTTGCAGTTGCGATCAGTGTCAG160460.12319123808269106No Hit
TGAGAATGTTAGGTTTGGGCGGCTGATGGGAAACTTGTCAATGACAAATC160260.1230376904844327No Hit
GGTCTTTTTCCAAGTTCTTGCTTATACCAAGGGAAGTAGTTTGAGGCACT159950.12279969170713222No Hit
ATGACAAATCCACTCTCATATGTGGCCTCAGAGCCCTGATTTGCAGTTGC159880.12274595004774179No Hit
TGACACTGGATCGTCAGGGAGGTTCCACGTTGACAGATATCCCTGCTTGG158310.12154060140141358No Hit
CGACTTGACACTGGATCGTCAGGGAGGTTCCACGTTGACAGATATCCCTG158070.12135634428350353No Hit
TATCGACTTGACACTGGATCGTCAGGGAGGTTCCACGTTGACAGATATCC157980.12128724786428725No Hit
CTCACAGTCAGAGTTGAGAATGTTAGGTTTGGGCGGCTGATGGGAAACTT156900.12045809083369206No Hit
CTTGACACTGGATCGTCAGGGAGGTTCCACGTTGACAGATATCCCTGCTT156740.12033525275508537No Hit
ATCCACTCTCATATGTGGCCTCAGAGCCCTGATTTGCAGTTGCGATCAGT156600.1202277694363045No Hit
GTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCA155890.11968267546248729No Hit
ACATCATGGTGACTTGGCTATCGACTTGACACTGGATCGTCAGGGAGGTT155870.11966732070266145No Hit
GAGAATGTTAGGTTTGGGCGGCTGATGGGAAACTTGTCAATGACAAATCC155660.11950609572449016No Hit
CAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCACA155040.11903009816988921No Hit
AGTGTCAGGCTCTGTCCAGGTTGCTGACGGTACCAGAACATCATGGTGAC154910.11893029223102128No Hit
GGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAGCCACAG153950.11819326375938108No Hit
CAGATATCCCTGCTTGGCTTTTGAGAGATGACAGCACTGAACACAGAGCC153520.1178631364231256No Hit
CTTCAGGGCTCATGTTGCTCACAGTCAGAGTTGAGAATGTTAGGTTTGGG153170.11759442812617343No Hit
CTCTGTCCAGGTTGCTGACGGTACCAGAACATCATGGTGACTTGGCTATC153080.11752533170695718No Hit
GGCTCATGTTGCTCACAGTCAGAGTTGAGAATGTTAGGTTTGGGCGGCTG149680.11491502253656487No Hit
TGGCTATCGACTTGACACTGGATCGTCAGGGAGGTTCCACGTTGACAGAT149560.11482289397760984No Hit
GAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGT149430.1147230880387419No Hit
GTTGACAGATATCCCTGCTTGGCTTTTGAGAGATGACAGCACTGAACACA149160.11451579878109311No Hit
AGTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAG148690.11415496192518594No Hit
CATATGTGGCCTCAGAGCCCTGATTTGCAGTTGCGATCAGTGTCAGGCTC148590.11407818812605673No Hit
GTTGAAGGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAG148190.11377109292954No Hit
CTCAGGGTTGAAGGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAG148150.11374038340988832No Hit
GAGAGATGACAGCACTGAACACAGAGCCTAGTCCCAGGAGAAGGAGCAGA147590.1133104501347649No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG147460.11321064419589695No Hit
GCCCCAGAAACCCCCTGTCCTTCAACGCTGCAGAGATATATGCTGCTGTC147040.11288819423955437No Hit
TGGGAAACTTGTCAATGACAAATCCACTCTCATATGTGGCCTCAGAGCCC146200.11224329432686922No Hit
GACACTGGATCGTCAGGGAGGTTCCACGTTGACAGATATCCCTGCTTGGC145800.11193619913035247No Hit
ACTGGATCGTCAGGGAGGTTCCACGTTGACAGATATCCCTGCTTGGCTTT145000.11132200873731898No Hit
GCACTGAACACAGAGCCTAGTCCCAGGAGAAGGAGCAGAAGACTCAGCAT144790.1111607837591477No Hit
GGCTATCGACTTGACACTGGATCGTCAGGGAGGTTCCACGTTGACAGATA144480.11092278498184723No Hit
GGGCGGCTGATGGGAAACTTGTCAATGACAAATCCACTCTCATATGTGGC142710.10956388873726063No Hit
GATTTGCAGTTGCGATCAGTGTCAGGCTCTGTCCAGGTTGCTGACGGTAC142640.10951014707787021No Hit
CACTCTCATATGTGGCCTCAGAGCCCTGATTTGCAGTTGCGATCAGTGTC141950.10898040786387882No Hit
CATCATGGTGACTTGGCTATCGACTTGACACTGGATCGTCAGGGAGGTTC141640.10874240908657834No Hit
GTAGTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTC141460.10860421624814581No Hit
CTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCT140370.1077673818376377No Hit
CACGTTGACAGATATCCCTGCTTGGCTTTTGAGAGATGACAGCACTGAAC140220.10765222113894393No Hit
TATGTGGCCTCAGAGCCCTGATTTGCAGTTGCGATCAGTGTCAGGCTCTG139970.10746028664112095No Hit
GGAGGTTCCACGTTGACAGATATCCCTGCTTGGCTTTTGAGAGATGACAG138940.10666951651009034No Hit
CTGGATCGTCAGGGAGGTTCCACGTTGACAGATATCCCTGCTTGGCTTTT138730.10650829153191907No Hit
ATGGGAAACTTGTCAATGACAAATCCACTCTCATATGTGGCCTCAGAGCC136790.10501887982881286No Hit
CCAAGGGAAGTAGTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAG136720.10496513816942242No Hit
CTTGGCTTTTGAGAGATGACAGCACTGAACACAGAGCCTAGTCCCAGGAG136260.10461197869342817No Hit
CACAGTCAGAGTTGAGAATGTTAGGTTTGGGCGGCTGATGGGAAACTTGT136190.10455823703403774No Hit
TGCAGTTGCGATCAGTGTCAGGCTCTGTCCAGGTTGCTGACGGTACCAGA135260.10384424070213631No Hit
GAGTTGAGAATGTTAGGTTTGGGCGGCTGATGGGAAACTTGTCAATGACA134710.10342198480692578No Hit
CCCTGCTTGGCTTTTGAGAGATGACAGCACTGAACACAGAGCCTAGTCCC134420.10319934078945114No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC134360.10315327650997365No Hit
ATTTGCAGTTGCGATCAGTGTCAGGCTCTGTCCAGGTTGCTGACGGTACC133620.10258515039641768No Hit
ATCGTCAGGGAGGTTCCACGTTGACAGATATCCCTGCTTGGCTTTTGAGA133190.10225502306016217No Hit
CTTCAACGCTGCAGAGATATATGCTGCTGTCTTCAGGGCTCATGTTGCTC132810.10196328262347128No Hit
GCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAGCCACAGGAATATA131740.10114180297278899No Hit
AGGGAGGTTCCACGTTGACAGATATCCCTGCTTGGCTTTTGAGAGATGAC131290.10079632087670765No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC130450.10015142096402249No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG904100.0137.20781
GTCTCTC898350.0118.667972
TCTCTCA911550.0116.6548163
CTCTCAG980150.0108.089984
TCTCAGC991100.0106.6104665
AGCTGGT1008700.0105.1869
TCAGCTG1011400.0105.031817
AGTCTCT1027550.0104.347261
CAGCTGG1022450.0103.2821668
CTCAGCT1075350.097.879976
GATGGCT1579650.077.729984
GGCTCAA1612250.076.4295357
GCTCAAA1634750.075.680928
ATGGCTC1614950.075.5415955
TGGCTCA1628950.075.35946
CTCAAAC1646650.075.252879
TCTCTGT962100.066.50635145
ACCCCTC89000.059.37556145
CCTTCCA97000.059.111908145
TCAAACA1644650.054.4660879
Produced by FastQC (version 0.11.5)