FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9020532
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC3783554.194375675403624No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3602933.994143582662309No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGG2495112.7660341984264343No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC2376582.63463396615632No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2166642.401898247243067No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGGA517990.574234424311116No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTC410890.45550528505414095No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGAAGAG397330.4404729122406528No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGGATG318290.352850585752592No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT248750.27575978889050007No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC247390.27425211728088766No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG175250.19427900704747791No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC161830.17940183572321455No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT158530.17574351490577275No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG131980.14631066105635454No Hit
CAAAGGACCACACAGCAGAGCACCTGGTTGCTCATAGTGCAAAGGGGGCC129180.14320663127185845No Hit
GGAAGAGAATGAGAAAAGAGACTTGGCTCGGGGGCTTCTCCTGGCCTAAT126040.1397256835849593No Hit
GAGTGATTCCACCATCCACGGTGTTTGCTCCCAGGAGACAAAGGACCACA122580.13588998963697485No Hit
GTCACATTCTGTCCTTCCTTTCTGAACAGGTACTTCGGGGACTGAGTGAT116730.12940478455150983No Hit
GTGCAAAGGGGGCCTTCTTGGAGCTCCAAGCACTTGTTGGAAGAGAATGA114990.1274758517568587No Hit
GGTACTTCGGGGACTGAGTGATTCCACCATCCACGGTGTTTGCTCCCAGG111230.12330758318910681No Hit
GTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCT102690.11384029234639377No Hit
GCAAAGGGGGCCTTCTTGGAGCTCCAAGCACTTGTTGGAAGAGAATGAGA100520.11143466926340928No Hit
CCAAGCACTTGTTGGAAGAGAATGAGAAAAGAGACTTGGCTCGGGGGCTT100420.11132381105682014No Hit
GCTCCAAGCACTTGTTGGAAGAGAATGAGAAAAGAGACTTGGCTCGGGGG99310.11009328496368065No Hit
GTTTGCTCCCAGGAGACAAAGGACCACACAGCAGAGCACCTGGTTGCTCA98770.10949465064809924No Hit
GGGCCTTCTTGGAGCTCCAAGCACTTGTTGGAAGAGAATGAGAAAAGAGA96310.10676753876600625No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA95670.10605804624383573No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC92700.10276555750813811No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)