FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11224296
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT5275094.699706778937405No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC5198984.631898517287855No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3782743.3701356414691843No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGC1847281.645786960714507No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTA1038990.9256616183322322No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACT626000.5577187201763032No Hit
GGGGCCCCTGACCCAGGACCTGTTGGTACCAGGACACACTCTTGTGCCCA377610.3364219902967634No Hit
GAAAGATAAACTGGGGCCCCTGACCCAGGACCTGTTGGTACCAGGACACA367230.32717419426572497No Hit
GGAGAGCATCTCAGAGTCACGTGCTGTCCTCTCGTTTTGATCAGGTGTGT362990.32339667449967463No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGCA358050.3189955076024367No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC354180.31554762989144264No Hit
GTGGGACTTTGGGTGACTCCAGCGTCCACTGGGCCTGCTCCCAGGAGACA330040.2940407131102031No Hit
GATAAACTGGGGCCCCTGACCCAGGACCTGTTGGTACCAGGACACACTCT328520.29268650791105294No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG328030.2922499549192217No Hit
GTCACGTGCTGTCCTCTCGTTTTGATCAGGTGTGTGGGACTTTGGGTGAC305820.2724625223711135No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT299470.26680515196676924No Hit
GGTGACTCCAGCGTCCACTGGGCCTGCTCCCAGGAGACAAAGCAGCACCC293490.2614774236174812No Hit
CCCAGGACCTGTTGGTACCAGGACACACTCTTGTGCCCACAGATAGGAGA282760.25191780402084907No Hit
AAACTGGGGCCCCTGACCCAGGACCTGTTGGTACCAGGACACACTCTTGT275830.2457436974220922No Hit
GTTTTGATCAGGTGTGTGGGACTTTGGGTGACTCCAGCGTCCACTGGGCC270810.2412712565670043No Hit
GCCCACAGATAGGAGAGCATCTCAGAGTCACGTGCTGTCCTCTCGTTTTG267210.23806392846375402No Hit
GGTACCAGGACACACTCTTGTGCCCACAGATAGGAGAGCATCTCAGAGTC263560.23481205413684744No Hit
ATAAACTGGGGCCCCTGACCCAGGACCTGTTGGTACCAGGACACACTCTT253880.2261879052369966No Hit
CACTCTTGTGCCCACAGATAGGAGAGCATCTCAGAGTCACGTGCTGTCCT249430.22222329133158997No Hit
GTTTCCTCTTCCTCTCTCTTCTTTCTCATAATACTGAAAGATAAACTGGG247810.22077999368512735No Hit
GGGACTTTGGGTGACTCCAGCGTCCACTGGGCCTGCTCCCAGGAGACAAA241890.21550572080422684No Hit
GTTGGTACCAGGACACACTCTTGTGCCCACAGATAGGAGAGCATCTCAGA238560.2125389423087203No Hit
GATAGGAGAGCATCTCAGAGTCACGTGCTGTCCTCTCGTTTTGATCAGGT233810.20830705106137615No Hit
GATCAGGTGTGTGGGACTTTGGGTGACTCCAGCGTCCACTGGGCCTGCTC232290.206952845862226No Hit
AACTGGGGCCCCTGACCCAGGACCTGTTGGTACCAGGACACACTCTTGTG230780.20560754990780716No Hit
GGTGTGTGGGACTTTGGGTGACTCCAGCGTCCACTGGGCCTGCTCCCAGG228560.20362969757746946No Hit
CTGAAAGATAAACTGGGGCCCCTGACCCAGGACCTGTTGGTACCAGGACA223600.19921071219076905No Hit
CCCACAGATAGGAGAGCATCTCAGAGTCACGTGCTGTCCTCTCGTTTTGA222500.19823069527033144No Hit
GCATCTCAGAGTCACGTGCTGTCCTCTCGTTTTGATCAGGTGTGTGGGAC220740.19666266819763126No Hit
CTCCAGCGTCCACTGGGCCTGCTCCCAGGAGACAAAGCAGCACCCAGCAG216820.19317024426298096No Hit
GGCCCCTGACCCAGGACCTGTTGGTACCAGGACACACTCTTGTGCCCACA214750.19132603060361203No Hit
GGCGAGCTGAGAATCGATCAGGGAAGTTTCCTCTTCCTCTCTCTTCTTTC212840.1896243648599431No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGCACG211630.18854634624746175No Hit
AGATAAACTGGGGCCCCTGACCCAGGACCTGTTGGTACCAGGACACACTC211280.188234522681868No Hit
ACCAGGACACACTCTTGTGCCCACAGATAGGAGAGCATCTCAGAGTCACG209630.18676449730121158No Hit
GCTCAGAGCTATAGTTAGGGAACTGGCGAGCTGAGAATCGATCAGGGAAG209070.18626557959626155No Hit
GATCAGGGAAGTTTCCTCTTCCTCTCTCTTCTTTCTCATAATACTGAAAG208350.18562411397561146No Hit
GTCCTCTCGTTTTGATCAGGTGTGTGGGACTTTGGGTGACTCCAGCGTCC208230.18551720303883648No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC205430.18302261451408625No Hit
GCCCTCTTAAACCGCTGACTGTGTAACTGCAGTTGAGACTGCTACTCTCT190500.1697211121303287No Hit
GGGAAGTTTCCTCTTCCTCTCTCTTCTTTCTCATAATACTGAAAGATAAA186710.16634450837718465No Hit
CCAGGACACACTCTTGTGCCCACAGATAGGAGAGCATCTCAGAGTCACGT185260.16505266789115328No Hit
AAGATAAACTGGGGCCCCTGACCCAGGACCTGTTGGTACCAGGACACACT183530.16351136855264686No Hit
CTCTCGTTTTGATCAGGTGTGTGGGACTTTGGGTGACTCCAGCGTCCACT182670.1627451735057593No Hit
CCTGTTGGTACCAGGACACACTCTTGTGCCCACAGATAGGAGAGCATCTC180400.16072277495176535No Hit
GTGCTGTCCTCTCGTTTTGATCAGGTGTGTGGGACTTTGGGTGACTCCAG178980.15945766219992774No Hit
CTTTGGGTGACTCCAGCGTCCACTGGGCCTGCTCCCAGGAGACAAAGCAG175240.1561256046704399No Hit
GGACTTTGGGTGACTCCAGCGTCCACTGGGCCTGCTCCCAGGAGACAAAG174910.15583159959430862No Hit
GTACCAGGACACACTCTTGTGCCCACAGATAGGAGAGCATCTCAGAGTCA173270.1543704834583835No Hit
CACGTGCTGTCCTCTCGTTTTGATCAGGTGTGTGGGACTTTGGGTGACTC167650.1493634879194205No Hit
GGAATTCAGGGCCTTTCCCAGGATCTTGCCTATACCAGAACAGCCCTCTT164990.1469936288209078No Hit
GCCTGCTCCCAGGAGACAAAGCAGCACCCAGCAGAGGAGCCCAGGGCCCA164010.1461205228372452No Hit
CCAGCGTCCACTGGGCCTGCTCCCAGGAGACAAAGCAGCACCCAGCAGAG161720.14408030579378875No Hit
CCCTGACCCAGGACCTGTTGGTACCAGGACACACTCTTGTGCCCACAGAT161410.14380411920711997No Hit
GTTGAGACTGCTACTCTCTCCCTCCTGGAGTCTCAGGGCCTCGGGACTCT161340.14374175449400123No Hit
CAAAGCAGCACCCAGCAGAGGAGCCCAGGGCCCATGGCACAGTGGGGCAG161280.14368829902561372No Hit
GGACACACTCTTGTGCCCACAGATAGGAGAGCATCTCAGAGTCACGTGCT159890.14244991400796986No Hit
GCCCCTGACCCAGGACCTGTTGGTACCAGGACACACTCTTGTGCCCACAG158120.14087297769053844No Hit
CCAGGACCTGTTGGTACCAGGACACACTCTTGTGCCCACAGATAGGAGAG156610.1395276817361196No Hit
GGGCCGAGTCCCCCAGCAACAAGGCGTTCACATTCAGCTCAGAGCTATAG156260.13921585817052579No Hit
CTCGTTTTGATCAGGTGTGTGGGACTTTGGGTGACTCCAGCGTCCACTGG153930.13714000414814437No Hit
ATCAGGTGTGTGGGACTTTGGGTGACTCCAGCGTCCACTGGGCCTGCTCC153530.13678363435889432No Hit
CACACTCTTGTGCCCACAGATAGGAGAGCATCTCAGAGTCACGTGCTGTC153120.13641835532491303No Hit
TAAACTGGGGCCCCTGACCCAGGACCTGTTGGTACCAGGACACACTCTTG152190.13558979556490672No Hit
GGGCCCCTGACCCAGGACCTGTTGGTACCAGGACACACTCTTGTGCCCAC151320.13481469127328788No Hit
CTCAGAGTCACGTGCTGTCCTCTCGTTTTGATCAGGTGTGTGGGACTTTG150820.13436922903672532No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC150800.13435141054726282No Hit
GCTGAGAATCGATCAGGGAAGTTTCCTCTTCCTCTCTCTTCTTTCTCATA148200.13203500691713763No Hit
CTCTTGTGCCCACAGATAGGAGAGCATCTCAGAGTCACGTGCTGTCCTCT147440.13135790431756256No Hit
AGAGCATCTCAGAGTCACGTGCTGTCCTCTCGTTTTGATCAGGTGTGTGG146520.13053825380228745No Hit
CCAGGATCTTGCCTATACCAGAACAGCCCTCTTAAACCGCTGACTGTGTA145700.1298076957343249No Hit
AGGACACACTCTTGTGCCCACAGATAGGAGAGCATCTCAGAGTCACGTGC144130.1284089443115185No Hit
GACACACTCTTGTGCCCACAGATAGGAGAGCATCTCAGAGTCACGTGCTG142590.12703692062290586No Hit
GGGCCTGCTCCCAGGAGACAAAGCAGCACCCAGCAGAGGAGCCCAGGGCC140020.12474724472697442No Hit
CTCAGGGCCTCGGGACTCTGCGTCACCTGGTCTTCTCCACTCAACCAGCC139530.12431069173514313No Hit
GCTATAGTTAGGGAACTGGCGAGCTGAGAATCGATCAGGGAAGTTTCCTC137580.12257338901254922No Hit
GAGTCACGTGCTGTCCTCTCGTTTTGATCAGGTGTGTGGGACTTTGGGTG136810.1218873771682429No Hit
GTTAGGGAACTGGCGAGCTGAGAATCGATCAGGGAAGTTTCCTCTTCCTC136810.1218873771682429No Hit
CCAGAACAGCCCTCTTAAACCGCTGACTGTGTAACTGCAGTTGAGACTGC135560.12077372157683654No Hit
ATCTCAGAGTCACGTGCTGTCCTCTCGTTTTGATCAGGTGTGTGGGACTT134900.12018571142457397No Hit
GTGCCCACAGATAGGAGAGCATCTCAGAGTCACGTGCTGTCCTCTCGTTT134870.12015898369038022No Hit
GAGAATCGATCAGGGAAGTTTCCTCTTCCTCTCTCTTCTTTCTCATAATA134320.11966897523016144No Hit
CTTGTGCCCACAGATAGGAGAGCATCTCAGAGTCACGTGCTGTCCTCTCG132680.11820785909423628No Hit
ACTGGGGCCCCTGACCCAGGACCTGTTGGTACCAGGACACACTCTTGTGC132500.11804749268907377No Hit
CGTTTTGATCAGGTGTGTGGGACTTTGGGTGACTCCAGCGTCCACTGGGC131500.11715656821594869No Hit
CATAATACTGAAAGATAAACTGGGGCCCCTGACCCAGGACCTGTTGGTAC130410.11618546054024234No Hit
GTCCACTGGGCCTGCTCCCAGGAGACAAAGCAGCACCCAGCAGAGGAGCC129960.11578454452733605No Hit
ACACACTCTTGTGCCCACAGATAGGAGAGCATCTCAGAGTCACGTGCTGT129410.11529453606711727No Hit
CTGTCCTCTCGTTTTGATCAGGTGTGTGGGACTTTGGGTGACTCCAGCGT128800.11475107213851096No Hit
GGGAACTGGCGAGCTGAGAATCGATCAGGGAAGTTTCCTCTTCCTCTCTC128790.11474216289377971No Hit
GGGTGACTCCAGCGTCCACTGGGCCTGCTCCCAGGAGACAAAGCAGCACC128470.11445706706237968No Hit
GAAGTTTCCTCTTCCTCTCTCTTCTTTCTCATAATACTGAAAGATAAACT127830.11388687539957963No Hit
CTGGGGCCCCTGACCCAGGACCTGTTGGTACCAGGACACACTCTTGTGCC127140.11327213751312332No Hit
GACCCAGGACCTGTTGGTACCAGGACACACTCTTGTGCCCACAGATAGGA126360.11257721642408576No Hit
CCCAGGAGACAAAGCAGCACCCAGCAGAGGAGCCCAGGGCCCATGGCACA125360.11168629195096066No Hit
GCTGTCCTCTCGTTTTGATCAGGTGTGTGGGACTTTGGGTGACTCCAGCG125050.11141010536429188No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCCTGCCAG124790.11117846500127936No Hit
GTGTAACTGCAGTTGAGACTGCTACTCTCTCCCTCCTGGAGTCTCAGGGC123480.1100113539414855No Hit
CCTCTCGTTTTGATCAGGTGTGTGGGACTTTGGGTGACTCCAGCGTCCAC123310.10985989678105423No Hit
GAGAGCATCTCAGAGTCACGTGCTGTCCTCTCGTTTTGATCAGGTGTGTG123280.1098331690468605No Hit
CAACCAGCCAAGCTGAAGCCACAAGACTATGAATGCACACTCCAACATTT123270.10982425980212923No Hit
ACTGAAAGATAAACTGGGGCCCCTGACCCAGGACCTGTTGGTACCAGGAC122890.1094857085023417No Hit
CCACAGATAGGAGAGCATCTCAGAGTCACGTGCTGTCCTCTCGTTTTGAT122400.1090491555105104No Hit
GAACTGGCGAGCTGAGAATCGATCAGGGAAGTTTCCTCTTCCTCTCTCTT121660.10838987140039785No Hit
CTTCTTTCTCATAATACTGAAAGATAAACTGGGGCCCCTGACCCAGGACC119660.10660802245414769No Hit
CACAGATAGGAGAGCATCTCAGAGTCACGTGCTGTCCTCTCGTTTTGATC117940.10507563236037254No Hit
ACTTTGGGTGACTCCAGCGTCCACTGGGCCTGCTCCCAGGAGACAAAGCA117900.10503999538144754No Hit
GACTTTGGGTGACTCCAGCGTCCACTGGGCCTGCTCCCAGGAGACAAAGC117090.1043183465582162No Hit
AGGGAAGTTTCCTCTTCCTCTCTCTTCTTTCTCATAATACTGAAAGATAA116970.1042114356214412No Hit
GTCTCAGGGCCTCGGGACTCTGCGTCACCTGGTCTTCTCCACTCAACCAG116680.10395306752423493No Hit
CTCTGCGTCACCTGGTCTTCTCCACTCAACCAGCCAAGCTGAAGCCACAA116310.10362342546917866No Hit
CACTGGGCCTGCTCCCAGGAGACAAAGCAGCACCCAGCAGAGGAGCCCAG116290.10360560697971613No Hit
GGGCCTTTCCCAGGATCTTGCCTATACCAGAACAGCCCTCTTAAACCGCT116200.10352542377713489No Hit
GACCTGTTGGTACCAGGACACACTCTTGTGCCCACAGATAGGAGAGCATC115970.10332051114831611No Hit
GAATCGATCAGGGAAGTTTCCTCTTCCTCTCTCTTCTTTCTCATAATACT115740.10311559851949735No Hit
GACTGCTACTCTCTCCCTCCTGGAGTCTCAGGGCCTCGGGACTCTGCGTC115490.10289286740121607No Hit
TGTTGGTACCAGGACACACTCTTGTGCCCACAGATAGGAGAGCATCTCAG115420.1028305026880973No Hit
CTTGCCTATACCAGAACAGCCCTCTTAAACCGCTGACTGTGTAACTGCAG114410.10193066897024097No Hit
TACCAGGACACACTCTTGTGCCCACAGATAGGAGAGCATCTCAGAGTCAC112830.10052300830270336No Hit
CCTGCTCCCAGGAGACAAAGCAGCACCCAGCAGAGGAGCCCAGGGCCCAT112790.10048737132377834No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)