FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9878567
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC3950743.99930475746128No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3264083.3042039397009706No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTT2954172.9904843485902357No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC2186182.213053775917094No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGTTGTTG1914151.9376798274486573No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC1699201.7200875390124901No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGG758490.7678137932353953No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTTA372610.37719033539986113No Hit
CTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACCTGCCCACA270200.2735214530609551No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGTTGTTGC249000.25206085052619476No Hit
GTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGAC237130.24004493769187374No Hit
GGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGG231890.23474052461252728No Hit
GTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTC220400.22310928295571614No Hit
GGTCTGAGTGACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGG219710.22241080108076405No Hit
GTCCAGAATTCTGCTCCACCTCCTTCTGTTGGCTCCAAACCCAGCTCAAC213760.2163876602750176No Hit
GTAATAAATCAGCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACA203490.20599141555652759No Hit
GGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACCTGCCCACAGG202030.20451346840083184No Hit
GCCCCATGCCTGGGTCTTGTCGATACCAGGACATGTATTCATGGTTCATA198790.20123364046627412No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT197200.19962409527616706No Hit
GTCTGAGTGACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGGC194820.19721483895386852No Hit
GCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACATGTATTCATGG190030.19236595753209954No Hit
AATAAATCAGCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACATG185150.1874259697788151No Hit
GTCGATACCAGGACATGTATTCATGGTTCATATCCTGGGCACACTGCAGT184510.1867781025324827No Hit
CCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACCTGCCCAC183970.18623146454338974No Hit
CATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTC176380.1785481639189166No Hit
CCAGAATTCTGCTCCACCTCCTTCTGTTGGCTCCAAACCCAGCTCAACTG176330.17849754929029688No Hit
GTTGGCTCCAAACCCAGCTCAACTGAAGCCACAGGATCACTAGTAAAACT172590.17471157506954196No Hit
GTCAGTGATACCAGCACCAATTGAGTAATAAATCAGCCTCAGCCCCATGC168750.17082437159154765No Hit
ATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGA167550.1696096205046744No Hit
GCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGA165370.16740282269685472No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC164770.1667954471534181No Hit
CCAGAATATTGTCTGTACCAGAAGAAGGACTGGGAACCTCGGTCACTGTA163830.1658438921353674No Hit
GTCTTGTCGATACCAGGACATGTATTCATGGTTCATATCCTGGGCACACT163490.16549971266075333No Hit
TGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACCTGCCCACAG161670.1636573401789956No Hit
GGCAATGGCTCCCTCTGGAACACTGAGGGGTCCAGAATTCTGCTCCACCT161150.16313094804135053No Hit
CACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGGCTGCACAGCAC160740.16271590808666886No Hit
CCAGGACATGTATTCATGGTTCATATCCTGGGCACACTGCAGTGTCATGC159660.16162263210848293No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTTACT158650.16060021661036464No Hit
ATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACCTGCCCACAGGAGA158480.1604281268730576No Hit
ATTCATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTC157100.15903116312315338No Hit
GAGTAATAAATCAGCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGA156620.1585452626884041No Hit
GCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAG155210.15711793016132805No Hit
GTGATACCAGCACCAATTGAGTAATAAATCAGCCTCAGCCCCATGCCTGG153240.15512371379371118No Hit
AATCAGCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACATGTATT151710.1535749061579478No Hit
GGCTCCAAACCCAGCTCAACTGAAGCCACAGGATCACTAGTAAAACTCTC151160.1530181452431309No Hit
CTCCAAACCCAGCTCAACTGAAGCCACAGGATCACTAGTAAAACTCTCAA150450.15229941751673093No Hit
GCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGG148140.14996102167449996No Hit
CTTGTCGATACCAGGACATGTATTCATGGTTCATATCCTGGGCACACTGC147930.14974844023429712No Hit
GGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACCTGCC147230.1490398354336211No Hit
GGAGAGACAAGGCTGCACAGCACAGGAGGCCGATGCTCATGGCAGATGCT146830.14863491840466336No Hit
CCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGGCTGCACAGCACAG146630.14843245989018447No Hit
ATCAGCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACATGTATTC145840.14763274875799293No Hit
GGAACACTGAGGGGTCCAGAATTCTGCTCCACCTCCTTCTGTTGGCTCCA145350.1471367253975197No Hit
GCTCCACCTCCTTCTGTTGGCTCCAAACCCAGCTCAACTGAAGCCACAGG143800.14556767191030845No Hit
CTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGAC143640.14540570509872536No Hit
GCCGACAGCAGCCTGAGCGGGAAATCCTCTGTGGTTGATCTGGAGACATT142100.14384677453723804No Hit
CCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCAC141410.14314829266228593No Hit
GTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGA140690.14241944201016202No Hit
GAGTGACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGGCTGCA139720.14143751821493947No Hit
GCCACAGGATCACTAGTAAAACTCTCAAGGATTTCATCATTCTTCTCTGC137840.13953440817883808No Hit
GTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTG137390.1390788765212606No Hit
CTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCT135580.13724662696522683No Hit
GATACCAGCACCAATTGAGTAATAAATCAGCCTCAGCCCCATGCCTGGGT133550.1351916730432663No Hit
CATGTATTCATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTC130710.13231676213766633No Hit
CTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACT130280.13188147633153674No Hit
GTTGAGAGAGGCAATGGCTCCCTCTGGAACACTGAGGGGTCCAGAATTCT129970.1315676656340945No Hit
GGGTCTGAGTGACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAG129630.13122348615948043No Hit
GGGGTCTGAGTGACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAA128560.13014033310701845No Hit
GATACCAGGACATGTATTCATGGTTCATATCCTGGGCACACTGCAGTGTC128400.12997836629543535No Hit
GTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGG128230.12980627655812832No Hit
CACTGGACCTGCCCACAGGAGAGACAAGGCTGCACAGCACAGGAGGCCGA126870.12842955865967198No Hit
GACAAGGCTGCACAGCACAGGAGGCCGATGCTCATGGCAGATGCTGCATG126490.12804488748216214No Hit
GCCTGAGCGGGAAATCCTCTGTGGTTGATCTGGAGACATTGTAGACATTG125850.12739702023582974No Hit
AGTAAAACTCTCAAGGATTTCATCATTCTTCTCTGCCCTGGTTCACTGAG124130.12565587701131145No Hit
CATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACAC123080.12459296981029738No Hit
GTGCAGTTGAGAGAGGCAATGGCTCCCTCTGGAACACTGAGGGGTCCAGA123070.12458284688457344No Hit
CCTGCCCACAGGAGAGACAAGGCTGCACAGCACAGGAGGCCGATGCTCAT121700.12319600606039316No Hit
GGGAACCTCGGTCACTGTAAGTGCAGTTGAGAGAGGCAATGGCTCCCTCT121610.12310489972887767No Hit
CCAGCACCAATTGAGTAATAAATCAGCCTCAGCCCCATGCCTGGGTCTTG120050.1215257233159425No Hit
CTTCTCCTTGGTCAGTGATACCAGCACCAATTGAGTAATAAATCAGCCTC119480.1209487165496777No Hit
GGGGACTTCTCCTTGGTCAGTGATACCAGCACCAATTGAGTAATAAATCA118950.12041220148630868No Hit
GGTTGATCTGGAGACATTGTAGACATTGGGGACTTCTCCTTGGTCAGTGA118550.12000728445735095No Hit
ATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACC117700.11914683577081574No Hit
GGAGACATTGTAGACATTGGGGACTTCTCCTTGGTCAGTGATACCAGCAC117360.11880265629620167No Hit
CCTGGGTCTTGTCGATACCAGGACATGTATTCATGGTTCATATCCTGGGC117280.11872167289041012No Hit
CCTTGGTCAGTGATACCAGCACCAATTGAGTAATAAATCAGCCTCAGCCC114600.11600872879639323No Hit
CACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGT113400.11479397770952002No Hit
GGACCTGCCCACAGGAGAGACAAGGCTGCACAGCACAGGAGGCCGATGCT112720.11410561876029185No Hit
GGACTGGGAACCTCGGTCACTGTAAGTGCAGTTGAGAGAGGCAATGGCTC111320.11268840915893977No Hit
GAGGCAATGGCTCCCTCTGGAACACTGAGGGGTCCAGAATTCTGCTCCAC110820.11218226287274258No Hit
GCTCCAAACCCAGCTCAACTGAAGCCACAGGATCACTAGTAAAACTCTCA110790.11215189409557073No Hit
ATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTG110520.11187857510102427No Hit
GTCTGTACCAGAAGAAGGACTGGGAACCTCGGTCACTGTAAGTGCAGTTG110440.11179759169523272No Hit
ATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCA109750.11109910982028061No Hit
CAAATTTTAAACCCTTAAAAAATGCACCTGGAATGGATATTTCTACTTTA109120.11046136549967217No Hit
GTGACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGGCTGCACA109020.11036013624243274No Hit
CTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACATGTATTCATGGTT108800.11013743187650597No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGTTGTTGCCA108080.10940858122438205No Hit
GCAATGGCTCCCTCTGGAACACTGAGGGGTCCAGAATTCTGCTCCACCTC107970.10929722904141866No Hit
GGTCCAGAATTCTGCTCCACCTCCTTCTGTTGGCTCCAAACCCAGCTCAA106460.10776866725710318No Hit
CTGAGTGACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGGCTG106200.10750547118828063No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105990.10729288974807784No Hit
GGCTTTTCCCAGAATATTGTCTGTACCAGAAGAAGGACTGGGAACCTCGG105970.10727264389662995No Hit
CAGAATTCTGCTCCACCTCCTTCTGTTGGCTCCAAACCCAGCTCAACTGA104530.10581494259238207No Hit
GACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGGCTGCACAGC104490.10577445088948628No Hit
CCAGAAGAAGGACTGGGAACCTCGGTCACTGTAAGTGCAGTTGAGAGAGG104200.10548088604349194No Hit
CCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAAT104070.10534928800908067No Hit
AGAAGAAGGACTGGGAACCTCGGTCACTGTAAGTGCAGTTGAGAGAGGCA103000.10426613495661871No Hit
CAAGGCTGCACAGCACAGGAGGCCGATGCTCATGGCAGATGCTGCATGAC102240.103496792601599No Hit
CGATACCAGGACATGTATTCATGGTTCATATCCTGGGCACACTGCAGTGT102030.10328421116139617No Hit
GGGACTTCTCCTTGGTCAGTGATACCAGCACCAATTGAGTAATAAATCAG101780.1030311380182976No Hit
AAATCAGCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACATGTAT101710.10296027753822999No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT101010.10225167273755394No Hit
GGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTG99770.10099642994778493No Hit
CCCAGCTCAACTGAAGCCACAGGATCACTAGTAAAACTCTCAAGGATTTC99440.1006623733988948No Hit
CTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATT99320.10054089829020747No Hit
GAGAGGCAATGGCTCCCTCTGGAACACTGAGGGGTCCAGAATTCTGCTCC99070.10028782514710889No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)