FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12031148
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG3538632.9412238965059694No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3469962.8841470489765397No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC2414592.006948962808869No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2292021.9050717354653104No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC1546231.2851890775510366No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG1290771.0728568878048876No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA767520.6379441097391537No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG635790.5284533113548266No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG510600.42439840321139766No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACC508640.42276929849088385No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG508290.4224783869336492No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG330830.2749779156569265No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC317390.26380691185911764No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG306160.2544728067512759No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG295100.2452800015426624No Hit
GTCACATTCTGTCCTTCCTTTCTGAACAGGTACTTTGGGGACTGAGTGAT281530.2340009448807379No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC278030.23109182930839187No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTG252110.20954775055547484No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTGA247660.2058490178992063No Hit
GGAAGAGAATGAGAAAAGAGACTTGGCTCGGGGGCTTCTCCTGGCCTAAT243300.20222509107194092No Hit
GTGCAAAGGGGGCCTTCTTGGAGCTCCAAGCACTTGTTGGAAGAGAATGA232800.19349774435490277No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT231750.19262500968319898No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCCTGCCAG225860.18772938376287948No Hit
GGTACTTTGGGGACTGAGTGATTCCACCATCCACGGTGTTTGCTCCCAGG223260.1855683264805653No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG223040.18538546778744638No Hit
GAGTGATTCCACCATCCACGGTGTTTGCTCCCAGGAAACAAAGGACCACA218480.18159530578461838No Hit
GTTCACAACTCAGGGTCACATTCTGTCCTTCCTTTCTGAACAGGTACTTT215530.17914333694506956No Hit
CAAAGGACCACACAGCAGAGCACCTGGTTACTCATAGTGCAAAGGGGGCC209780.17436407564764392No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGG208840.17358277032249958No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC208630.1734082233881588No Hit
GTTTGCTCCCAGGAAACAAAGGACCACACAGCAGAGCACCTGGTTACTCA206030.1712471661058446No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA202400.16822999766938285No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA198140.1646891884298988No Hit
GCAAAGGGGGCCTTCTTGGAGCTCCAAGCACTTGTTGGAAGAGAATGAGA196760.16354216571851662No Hit
GCTCCAAGCACTTGTTGGAAGAGAATGAGAAAAGAGACTTGGCTCGGGGG194710.16183825516899966No Hit
CCAAGCACTTGTTGGAAGAGAATGAGAAAAGAGACTTGGCTCGGGGGCTT194610.16175513758121834No Hit
GGGCCTTCTTGGAGCTCCAAGCACTTGTTGGAAGAGAATGAGAAAAGAGA188840.15695925276623643No Hit
CCCAGGAAACAAAGGACCACACAGCAGAGCACCTGGTTACTCATAGTGCA185570.15424130764578742No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTGACC173690.14436693821736712No Hit
CCACCATCCACGGTGTTTGCTCCCAGGAAACAAAGGACCACACAGCAGAG172080.14302874505408794No Hit
CTTGGCTCGGGGGCTTCTCCTGGCCTAATGTGCAGAGTTTGTCAGAGGCT167910.13956274164360707No Hit
GGGTCACATTCTGTCCTTCCTTTCTGAACAGGTACTTTGGGGACTGAGTG166310.13823286023910603No Hit
GGACCACACAGCAGAGCACCTGGTTACTCATAGTGCAAAGGGGGCCTTCT165360.13744324315518353No Hit
CATAGTGCAAAGGGGGCCTTCTTGGAGCTCCAAGCACTTGTTGGAAGAGA165040.1371772668742833No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCCTA164430.1366702495888173No Hit
CAGCAGAGCACCTGGTTACTCATAGTGCAAAGGGGGCCTTCTTGGAGCTC160480.1333871048714553No Hit
CTCCCAGGAAACAAAGGACCACACAGCAGAGCACCTGGTTACTCATAGTG159720.13275541120431733No Hit
AGAAAAGAGACTTGGCTCGGGGGCTTCTCCTGGCCTAATGTGCAGAGTTT159460.13253930547608592No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA157560.13096007130824092No Hit
GCTTCAAATTCTGTTCACAACTCAGGGTCACATTCTGTCCTTCCTTTCTG157340.130777212615122No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT157230.1306857832685626No Hit
GAATGAGAAAAGAGACTTGGCTCGGGGGCTTCTCCTGGCCTAATGTGCAG152950.12712835051152227No Hit
GTTTGTCAGAGGCTGGAATGAAGAGGTGGGAGGTTCCTGGAGATAATCAA152550.126795880160397No Hit
GGTCAATTCACCAGCCTTATATAAGGCTATCAAGAGGACAGGACCTTCCC150800.12534132237422396No Hit
GCCTTCTTGGAGCTCCAAGCACTTGTTGGAAGAGAATGAGAAAAGAGACT149740.12446027594374204No Hit
GTCCTTCCTTTCTGAACAGGTACTTTGGGGACTGAGTGATTCCACCATCC148310.12327169443846922No Hit
ATGAGAAAAGAGACTTGGCTCGGGGGCTTCTCCTGGCCTAATGTGCAGAG145480.12091946670425799No Hit
GTTAAATATGCTTGAAGAAGTGCAGTTCATGGAGACATCTTCTCCTTCCT144270.11991374389210406No Hit
GCAGAGCACCTGGTTACTCATAGTGCAAAGGGGGCCTTCTTGGAGCTCCA142630.11855061545249049No Hit
CCTGGTTACTCATAGTGCAAAGGGGGCCTTCTTGGAGCTCCAAGCACTTG142130.11813502751358392No Hit
GATTCCACCATCCACGGTGTTTGCTCCCAGGAAACAAAGGACCACACAGC141900.11794385706168688No Hit
CTTCCTTTCTGAACAGGTACTTTGGGGACTGAGTGATTCCACCATCCACG141610.11770281605712107No Hit
CTCATAGTGCAAAGGGGGCCTTCTTGGAGCTCCAAGCACTTGTTGGAAGA141030.11722073404798942No Hit
CTCTGATTCAGCTGTTGACTCTGATTCAGCTGTTGACCACTGACCCATGT140150.11648929927551385No Hit
CTCTGATTCAGCTGTTGACCACTGACCCATGTCAGCTGCATCCACAAGAT138890.1154420176694693No Hit
GATCAATCTCAGCCCTTGCCCTGGGTCCTGTCGGTACCAGTACATGGCAT137990.11469395937943744No Hit
GCCAGGTGTTAAATATGCTTGAAGAAGTGCAGTTCATGGAGACATCTTCT137710.11446123013364976No Hit
GTGCAGAGTTTGTCAGAGGCTGGAATGAAGAGGTGGGAGGTTCCTGGAGA137520.11430330671686526No Hit
CCTTCTTGGAGCTCCAAGCACTTGTTGGAAGAGAATGAGAAAAGAGACTT137140.11398745988329625No Hit
GGACTGAGTGATTCCACCATCCACGGTGTTTGCTCCCAGGAAACAAAGGA136920.11380460119017737No Hit
GTCCTGTCGGTACCAGTACATGGCATCGTGCTTCAAATTCTGTTCACAAC136560.11350537787416462No Hit
GGCTTCTCCTGGCCTAATGTGCAGAGTTTGTCAGAGGCTGGAATGAAGAG135760.11284043717191411No Hit
ACTCAGGGTCACATTCTGTCCTTCCTTTCTGAACAGGTACTTTGGGGACT135560.11267420199635148No Hit
ACACAGCAGAGCACCTGGTTACTCATAGTGCAAAGGGGGCCTTCTTGGAG135510.11263264320246082No Hit
GAAGAGAATGAGAAAAGAGACTTGGCTCGGGGGCTTCTCCTGGCCTAATG134940.1121588729521073No Hit
GTCAGAGGCTGGAATGAAGAGGTGGGAGGTTCCTGGAGATAATCAAGACA134910.11213393767577293No Hit
GGTACCAGTACATGGCATCGTGCTTCAAATTCTGTTCACAACTCAGGGTC134120.11147730873230052No Hit
GCACCTGGTTACTCATAGTGCAAAGGGGGCCTTCTTGGAGCTCCAAGCAC133680.11111159134606274No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGA133630.11107003255217207No Hit
AAATTCTGTTCACAACTCAGGGTCACATTCTGTCCTTCCTTTCTGAACAG133360.11084561506516254No Hit
GTGTTTGCTCCCAGGAAACAAAGGACCACACAGCAGAGCACCTGGTTACT133200.11071262692471243No Hit
CAGGAAACAAAGGACCACACAGCAGAGCACCTGGTTACTCATAGTGCAAA131570.10935781024387697No Hit
GACTTGGCTCGGGGGCTTCTCCTGGCCTAATGTGCAGAGTTTGTCAGAGG130790.10870949305918272No Hit
GCCCTTGCCCTGGGTCCTGTCGGTACCAGTACATGGCATCGTGCTTCAAA130610.10855988140117635No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCC130520.10848507557217316No Hit
GGGACTGAGTGATTCCACCATCCACGGTGTTTGCTCCCAGGAAACAAAGG128760.10702220602722201No Hit
ATTCCACCATCCACGGTGTTTGCTCCCAGGAAACAAAGGACCACACAGCA128050.10643207115397467No Hit
GCTCCCAGGAAACAAAGGACCACACAGCAGAGCACCTGGTTACTCATAGT127550.1060164832150681No Hit
GCAGAGTTTGTCAGAGGCTGGAATGAAGAGGTGGGAGGTTCCTGGAGATA127490.10596661266239929No Hit
CACAACTCAGGGTCACATTCTGTCCTTCCTTTCTGAACAGGTACTTTGGG127460.1059416773860649No Hit
GGACCTTCCCCAGGGTCCTGCTTGTACCATAGCCAGGTGTTAAATATGCT127350.10585024803950545No Hit
GAACAGGTACTTTGGGGACTGAGTGATTCCACCATCCACGGTGTTTGCTC126160.10486114874490779No Hit
CTGAGTGATTCCACCATCCACGGTGTTTGCTCCCAGGAAACAAAGGACCA125440.10426270211288233No Hit
GGGGGCCTTCTTGGAGCTCCAAGCACTTGTTGGAAGAGAATGAGAAAAGA125150.10402166110831651No Hit
CACGGTGTTTGCTCCCAGGAAACAAAGGACCACACAGCAGAGCACCTGGT124830.10375568482741629No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTT123730.10284139136182184No Hit
AAAGAGACTTGGCTCGGGGGCTTCTCCTGGCCTAATGTGCAGAGTTTGTC121990.10139514533442694No Hit
GGAAACAAAGGACCACACAGCAGAGCACCTGGTTACTCATAGTGCAAAGG120470.10013175800015094No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)