FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14950364
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC6553364.383411668103867No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT5359783.5850498355759095No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC5006083.348466967091905No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC3109642.0799761129561793No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA2766311.8503295304381888No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC2491601.6665814959421723No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTTA975290.6523520096233109No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTTACT569960.38123486491700137No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAA512790.3429949932991598No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT360220.2409439663141312No Hit
GTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACAG335200.22420858783103875No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC324360.21695792824843596No Hit
CTGAGATCTGACCCCAAAGAAAGTGCCCAGGAGAAGGGCAAGGAGAGAGC323670.2164964010240821No Hit
GTCTGAGATCTGACCCCAAAGAAAGTGCCCAGGAGAAGGGCAAGGAGAGA320870.2146235369252548No Hit
CTCAAGGCCTGAGGATCCTCTTCTCCCTGTTGACTGTTCACCCTAGCCAG314550.21039621510218748No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACA308300.20621571488159085No Hit
GTGGCATTTTCACCCTCCTGGATGCTCAAGGCCTGAGGATCCTCTTCTCC296170.19810220005345688No Hit
GCTGGCCATTGATGAATAGTCTGAGATCTGACCCCAAAGAAAGTGCCCAG295190.19744669761886735No Hit
GGCTGCACCAGGGTCGCTGGCCATTGATGAATAGTCTGAGATCTGACCCC283250.18946026999743953No Hit
GATGAATAGTCTGAGATCTGACCCCAAAGAAAGTGCCCAGGAGAAGGGCA272450.18223636561624854No Hit
GGTTGGGGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTG256560.17160786185540366No Hit
GCCCACAGGCTGCACCAGGGTCGCTGGCCATTGATGAATAGTCTGAGATC247760.1657217175448036No Hit
GTTTATACTAGTTTTGTAACTGCAGTTCATGGTGGCATTTTCACCCTCCT239270.1600429260451451No Hit
GGCATTTTCACCCTCCTGGATGCTCAAGGCCTGAGGATCCTCTTCTCCCT232000.1551801681885471No Hit
GCCCAGGAGAAGGGCAAGGAGAGAGCAGAGCATCATCCAAGCCAGCCTTT226450.1514678839926573No Hit
CCCCAAAGAAAGTGCCCAGGAGAAGGGCAAGGAGAGAGCAGAGCATCATC226030.15118695437783322No Hit
GCATCATCCAAGCCAGCCTTTCCTTCAGCTAGTCTGGGGGACAGACGCCT224340.15005654711818386No Hit
CATTTTCACCCTCCTGGATGCTCAAGGCCTGAGGATCCTCTTCTCCCTGT223990.14982243910583046No Hit
GAGCAGAGCATCATCCAAGCCAGCCTTTCCTTCAGCTAGTCTGGGGGACA223220.14930740147865296No Hit
CCTGAATTTTGTCTATACCACTGTAAATTGTTTATACTAGTTTTGTAACT220460.14746129258123747No Hit
GTTCATGGTGGCATTTTCACCCTCCTGGATGCTCAAGGCCTGAGGATCCT220440.14744791498053159No Hit
ATCCAAGCCAGCCTTTCCTTCAGCTAGTCTGGGGGACAGACGCCTCCTCT217780.14566869408664565No Hit
GCAAGGAGAGAGCAGAGCATCATCCAAGCCAGCCTTTCCTTCAGCTAGTC217770.1456620052862927No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG214470.14345470116981768No Hit
GTTCACCCTAGCCAGTTGAAGCCATAGAATCACCAAAGACACTCCCAGGA207080.1385116777089842No Hit
GGCAAGGAGAGAGCAGAGCATCATCCAAGCCAGCCTTTCCTTCAGCTAGT202310.13532111994062487No Hit
GGCCATTGATGAATAGTCTGAGATCTGACCCCAAAGAAAGTGCCCAGGAG202190.1352408543363894No Hit
CTAGTTTTGTAACTGCAGTTCATGGTGGCATTTTCACCCTCCTGGATGCT199330.13332785743544437No Hit
GGGCAAGGAGAGAGCAGAGCATCATCCAAGCCAGCCTTTCCTTCAGCTAG198730.1329265294142671No Hit
GGTGGACAAGGCCTCTACCTGAATTTTGTCTATACCACTGTAAATTGTTT198220.13258540059626642No Hit
GGCCTGAGGATCCTCTTCTCCCTGTTGACTGTTCACCCTAGCCAGTTGAA198030.13245831338956027No Hit
CCCAGGAGAAGGGCAAGGAGAGAGCAGAGCATCATCCAAGCCAGCCTTTC197550.13213725097261847No Hit
GTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACAGGCTGCAC195180.1305520052889682No Hit
GGATCCTCTTCTCCCTGTTGACTGTTCACCCTAGCCAGTTGAAGCCATAG193810.12963563964061342No Hit
GCAGAGCATCATCCAAGCCAGCCTTTCCTTCAGCTAGTCTGGGGGACAGA193070.12914066841449479No Hit
CTGGCCATTGATGAATAGTCTGAGATCTGACCCCAAAGAAAGTGCCCAGG190980.12774270914072727No Hit
GTTGGGGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGC189230.1265721690789602No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT188360.12599024344825316No Hit
GCCTGAGGATCCTCTTCTCCCTGTTGACTGTTCACCCTAGCCAGTTGAAG188300.12595011064613543No Hit
GTCTATACCACTGTAAATTGTTTATACTAGTTTTGTAACTGCAGTTCATG187340.1253079858122518No Hit
GCCATTGATGAATAGTCTGAGATCTGACCCCAAAGAAAGTGCCCAGGAGA187190.12520765380695748No Hit
GCCATAGAATCACCAAAGACACTCCCAGGAGAGTTTCCATTCTTCTTCCT186770.12492672419213338No Hit
CCCTCCTGGATGCTCAAGGCCTGAGGATCCTCTTCTCCCTGTTGACTGTT184080.1231274368971886No Hit
GGCTGCCCACAGGCTGCACCAGGGTCGCTGGCCATTGATGAATAGTCTGA182080.12178967682659766No Hit
GATCTGACCCCAAAGAAAGTGCCCAGGAGAAGGGCAAGGAGAGAGCAGAG182070.12178298802624471No Hit
CTCTTCTCCCTGTTGACTGTTCACCCTAGCCAGTTGAAGCCATAGAATCA181710.12154219121353835No Hit
GGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACA179240.11989005752635856No Hit
GCCTGTCGGTACCAGTATAGGTTGGGGTTTGATGTTCCCTCCACAGTGCA177570.11877302786741514No Hit
GTAAATTGTTTATACTAGTTTTGTAACTGCAGTTCATGGTGGCATTTTCA177340.11861918545929717No Hit
GCCAGTTGAAGCCATAGAATCACCAAAGACACTCCCAGGAGAGTTTCCAT173200.11585002211317397No Hit
CCCAAAGAAAGTGCCCAGGAGAAGGGCAAGGAGAGAGCAGAGCATCATCC173190.11584333331282101No Hit
GTTTTGTAACTGCAGTTCATGGTGGCATTTTCACCCTCCTGGATGCTCAA173150.11581657811140919No Hit
AGGGCAAGGAGAGAGCAGAGCATCATCCAAGCCAGCCTTTCCTTCAGCTA171180.11449888444187714No Hit
CAAAGAAAGTGCCCAGGAGAAGGGCAAGGAGAGAGCAGAGCATCATCCAA170500.11404404601787622No Hit
GTCGCTGGCCATTGATGAATAGTCTGAGATCTGACCCCAAAGAAAGTGCC168930.11299390436246234No Hit
GGTACCAGTATAGGTTGGGGTTTGATGTTCCCTCCACAGTGCACTCCAGA168280.1125591323395203No Hit
CTCCAGAGAGAGCGGGCTGCCCACAGGCTGCACCAGGGTCGCTGGCCATT167970.1123517795285787No Hit
CAAGGCCTGAGGATCCTCTTCTCCCTGTTGACTGTTCACCCTAGCCAGTT167160.11180998669998937No Hit
CTGAATTTTGTCTATACCACTGTAAATTGTTTATACTAGTTTTGTAACTG165550.1107330898431637No Hit
GAATAGTCTGAGATCTGACCCCAAAGAAAGTGCCCAGGAGAAGGGCAAGG162010.10836525451821774No Hit
GACTGTTCACCCTAGCCAGTTGAAGCCATAGAATCACCAAAGACACTCCC161940.10831843291574707No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC161770.10820472330974684No Hit
ACCAAAGACACTCCCAGGAGAGTTTCCATTCTTCTTCCTTATTGAAATCA161090.10774988488574593No Hit
CCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACAGGCTGCACCAGG160550.10738868966668638No Hit
CCAGAGAGAGCGGGCTGCCCACAGGCTGCACCAGGGTCGCTGGCCATTGA160450.10732180166315682No Hit
GGCCAATACCAACGGAGTAGAAGAGCAGCTGGAGGCCCCTGCCTGCAGCC160300.1072214696578625No Hit
GTCAAGCGTGACTCTTAATCTTCCACTGTGTTTCTCTCTTTCATTTGAAC159690.10681345283633228No Hit
GCCGTGATCAACAAGGAACTGCTTTTCTTGGAAGTGTCAAGCGTGACTCT159520.10669974323033206No Hit
GATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACAGGCTG158340.10591046478868339No Hit
GCCAGCCTTTCCTTCAGCTAGTCTGGGGGACAGACGCCTCCTCTTCTGGG157110.10508774234526999No Hit
GGGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCAC154850.10357607346550225No Hit
GGCCCCTGCCTGCAGCCTGTCGGTACCAGTATAGGTTGGGGTTTGATGTT154290.10320150064573679No Hit
GACGCCTCCTCTTCTGGGCCAGTGATGTGGTCAGGCTGCTCCCTCTGGGA153580.102726595820677No Hit
CCTGAGGATCCTCTTCTCCCTGTTGACTGTTCACCCTAGCCAGTTGAAGC153400.10260619741432384No Hit
CTGCAGTTCATGGTGGCATTTTCACCCTCCTGGATGCTCAAGGCCTGAGG151960.10164301016349836No Hit
GTAACTGCAGTTCATGGTGGCATTTTCACCCTCCTGGATGCTCAAGGCCT151300.10120154934020337No Hit
GTTGACTGTTCACCCTAGCCAGTTGAAGCCATAGAATCACCAAAGACACT149780.10018485168655426No Hit
ATTTTGTCTATACCACTGTAAATTGTTTATACTAGTTTTGTAACTGCAGT149720.10014471888443652No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG1156750.0134.534411
AGTCTCT1194750.0134.436461
GTCTCTC1339700.0119.413972
TCTCTCA1388150.0115.1255953
TCAGCTG1540200.0104.114737
AGCTGGT1586100.0101.145619
TCTCAGC1667150.095.745785
CTCTCAG1674250.095.473994
CTCAGCT1802650.088.608876
CTGATGG1987900.078.091142
TGATGGC2050300.075.558633
ACCCCTC214200.073.67151145
GGCTCAA2103450.073.59877
GATGGCT2111650.073.0850754
CTCAAAC2131700.072.873579
GCTCAAA2130200.072.802368
ATGGCTC2116550.072.6487055
CAGCTGG2253500.071.672778
TGATCAA1130850.070.73396145
GATATGA444400.069.63262145
Produced by FastQC (version 0.11.5)