FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11005527
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG5414854.9201187730492135No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3607593.277980236657454No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC2840442.5809213861362568No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2466822.2414374159456423No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC2099871.908014036947072No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTGA1103501.0026780180540196No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTGACC701420.6373343139315364No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTG522990.4752066847866531No Hit
GTGAGATTTTCTCCCTCTTGGATGCTTAGAAACTGAGGGCTCTGCTCCAG310050.28172208382206504No Hit
CTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACCTGCCCACA309980.28165847941675126No Hit
GGAAAAAACACTTGAGGAGTTGCAGTACACAGTGAGATTTTCTCCCTCTT278690.2532273102414814No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG269100.24451350671349042No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC259560.23584513490358072No Hit
GGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGG256520.2330828864442384No Hit
GTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGAC255340.23201069789752005No Hit
GTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTC233460.21212977806514854No Hit
CTTAGAAACTGAGGGCTCTGCTCCAGCAGCTGGGTGCTCACCCATGCCAA232140.21093038070780254No Hit
GGTCTGAGTGACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGG225030.20446999039664343No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT224770.20423374546262074No Hit
GGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACCTGCCCACAGG223390.2029798300435772No Hit
GGACCTTCCCCAGGCTCCTGTCTGTACCATTGTAAGCTGGAAAAAACACT222980.20260728995531063No Hit
GCCCCATGCCTGGGTCTTGTCGATACCAGGACATGTATTCATGGTTCATA216920.19710096572385857No Hit
GCTGGAAAAAACACTTGAGGAGTTGCAGTACACAGTGAGATTTTCTCCCT212170.19278495250613623No Hit
CTCTGCTCCAGCAGCTGGGTGCTCACCCATGCCAACTGAATCCAAAGAAT209960.1907768705669433No Hit
GTTGCAGTACACAGTGAGATTTTCTCCCTCTTGGATGCTTAGAAACTGAG206320.18746944149062558No Hit
GTCGATACCAGGACATGTATTCATGGTTCATATCCTGGGCACACTGCAGT206260.18741492342892804No Hit
GAGATTTTCTCCCTCTTGGATGCTTAGAAACTGAGGGCTCTGCTCCAGCA205920.18710598774597526No Hit
CCAGCAGCTGGGTGCTCACCCATGCCAACTGAATCCAAAGAATGGACACG205790.18698786527896394No Hit
GTCTGAGTGACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGGC195710.17782883091377633No Hit
AATGAATCAGCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACATG195040.17722004589148707No Hit
CAAAGAATGGACACGGAGAATTTCAGGACCATCTTGTCTTTCTATCACAT193030.1753936908246193No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC191690.17417612078004077No Hit
GTAATGAATCAGCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACA191070.17361276747583282No Hit
GCTCCAGCAGCTGGGTGCTCACCCATGCCAACTGAATCCAAAGAATGGAC189980.17262235602166076No Hit
GGACCATCTTGTCTTTCTATCACATGGTGGACATGGCCCCTGACTTTAGC186690.16963294897191203No Hit
CCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACCTGCCCAC184330.16748857187847524No Hit
CATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTC183350.166598110204082No Hit
GCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGA180960.16442647407979646No Hit
GCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACATGTATTCATGG179410.16301809081927654No Hit
GCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAG178550.16223666526827837No Hit
GGGCTCTGCTCCAGCAGCTGGGTGCTCACCCATGCCAACTGAATCCAAAG176540.1604103102014106No Hit
ATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGA175790.1597288344301913No Hit
GTCAGTGATACCAGCACCAACTGAGTAATGAATCAGCCTCAGCCCCATGC175100.15910187672066953No Hit
ATCAGCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACATGTATTC172930.15713014015594165No Hit
ATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACCTGCCCACAGGAGA171400.15573992958265423No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT171200.1555582027103291No Hit
GCTGGGTGCTCACCCATGCCAACTGAATCCAAAGAATGGACACGGAGAAT168070.15271417715844046No Hit
CTCACCCATGCCAACTGAATCCAAAGAATGGACACGGAGAATTTCAGGAC166090.1509150811224215No Hit
CCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGGCTGCACAGCACAG164900.14983380623208684No Hit
CTTGTCGATACCAGGACATGTATTCATGGTTCATATCCTGGGCACACTGC164900.14983380623208684No Hit
ATTCATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTC164840.1497792881703893No Hit
GTCACCAGGAGGACAGGACCTTCCCCAGGCTCCTGTCTGTACCATTGTAA164190.14918867583533257No Hit
CCAGGACATGTATTCATGGTTCATATCCTGGGCACACTGCAGTGTCATGC163680.1487252723109034No Hit
GGAGAGACAAGGCTGCACAGCACAGGAGGCCGATGCTCATGGCAGATGCT163130.14822552341200926No Hit
CACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGGCTGCACAGCAC162410.14757130667163873No Hit
CCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCAC160640.14596302385156112No Hit
TGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACCTGCCCACAG159320.14476362649421512No Hit
GTGATACCAGCACCAACTGAGTAATGAATCAGCCTCAGCCCCATGCCTGG157940.1435097110751716No Hit
GCAGTACACAGTGAGATTTTCTCCCTCTTGGATGCTTAGAAACTGAGGGC156570.1422648819997443No Hit
GCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGG155760.14152888816682743No Hit
GTCTTGTCGATACCAGGACATGTATTCATGGTTCATATCCTGGGCACACT154820.14067477186689925No Hit
GGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACCTGCC154570.1404476132764928No Hit
GTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGA154430.1403204044658652No Hit
GTAAGCTGGAAAAAACACTTGAGGAGTTGCAGTACACAGTGAGATTTTCT153850.13979339653612227No Hit
GCTCTGCTCCAGCAGCTGGGTGCTCACCCATGCCAACTGAATCCAAAGAA152150.13824871812135847No Hit
GAGTGACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGGCTGCA151020.13722196129272138No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA149240.13560459212902753No Hit
GCCGACAGCAGCCTGAGCGGGAAATCCTCTGTGGTTGATCTGGAGACATT149030.13541377891308612No Hit
CCCTCTTGGATGCTTAGAAACTGAGGGCTCTGCTCCAGCAGCTGGGTGCT148840.13524113838437724No Hit
CCAGCACCAACTGAGTAATGAATCAGCCTCAGCCCCATGCCTGGGTCTTG148530.13495946173227324No Hit
CTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCT147290.1338327551238573No Hit
GGACAGGACCTTCCCCAGGCTCCTGTCTGTACCATTGTAAGCTGGAAAAA145340.1320609181186871No Hit
GATTTTCTCCCTCTTGGATGCTTAGAAACTGAGGGCTCTGCTCCAGCAGC145250.13197914102614078No Hit
CCTTCCCCAGGCTCCTGTCTGTACCATTGTAAGCTGGAAAAAACACTTGA143470.13036177186244693No Hit
GTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTG142160.1291714608487172No Hit
GAGGGCTCTGCTCCAGCAGCTGGGTGCTCACCCATGCCAACTGAATCCAA141990.12901699300724082No Hit
CTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGAC141920.128953388601927No Hit
GATACCAGCACCAACTGAGTAATGAATCAGCCTCAGCCCCATGCCTGGGT141670.12872623001152056No Hit
CCATTGTAAGCTGGAAAAAACACTTGAGGAGTTGCAGTACACAGTGAGAT140650.1277994229626623No Hit
GAGTAATGAATCAGCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGA139990.12719972428398932No Hit
GACAAGGCTGCACAGCACAGGAGGCCGATGCTCATGGCAGATGCTGCATG139930.12714520622229175No Hit
CCCATGCCAACTGAATCCAAAGAATGGACACGGAGAATTTCAGGACCATC138120.12550057802774914No Hit
CTGGAAAAAACACTTGAGGAGTTGCAGTACACAGTGAGATTTTCTCCCTC136960.12444656216826329No Hit
CATGTATTCATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTC136530.12405584939276419No Hit
GTACCATTGTAAGCTGGAAAAAACACTTGAGGAGTTGCAGTACACAGTGA136140.12370148199173016No Hit
CTCCAGCAGCTGGGTGCTCACCCATGCCAACTGAATCCAAAGAATGGACA135700.12330168287261482No Hit
GGGTGCTCACCCATGCCAACTGAATCCAAAGAATGGACACGGAGAATTTC135700.12330168287261482No Hit
GAATCCAAAGAATGGACACGGAGAATTTCAGGACCATCTTGTCTTTCTAT135280.122920056440732No Hit
GGGTCTGAGTGACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAG135010.12267472516309305No Hit
CCTGCCCACAGGAGAGACAAGGCTGCACAGCACAGGAGGCCGATGCTCAT133540.1213390326515032No Hit
CACTGGACCTGCCCACAGGAGAGACAAGGCTGCACAGCACAGGAGGCCGA133060.12090288815792283No Hit
GGAGAATTTCAGGACCATCTTGTCTTTCTATCACATGGTGGACATGGCCC132710.12058486613135383No Hit
CATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACAC132570.12045765732072622No Hit
GGGGACTTCTCCTTGGTCAGTGATACCAGCACCAACTGAGTAATGAATCA131930.11987613132928573No Hit
GATACCAGGACATGTATTCATGGTTCATATCCTGGGCACACTGCAGTGTC131920.11986704498566947No Hit
CTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACT131700.11966714542611182No Hit
GTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGG131540.11952176392825169No Hit
GTACACAGTGAGATTTTCTCCCTCTTGGATGCTTAGAAACTGAGGGCTCT131280.11928551899422898No Hit
GAATCAGCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACATGTAT130540.11861312956662592No Hit
GGGGTCTGAGTGACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAA130510.11858587053577717No Hit
ATTTCAGGACCATCTTGTCTTTCTATCACATGGTGGACATGGCCCCTGAC130390.11847683441238208No Hit
GGAGACATTGTAGCCATTGGGGACTTCTCCTTGGTCAGTGATACCAGCAC130090.11820424410389434No Hit
GCCTGAGCGGGAAATCCTCTGTGGTTGATCTGGAGACATTGTAGCCATTG129620.11777718595393023No Hit
CACCCATGCCAACTGAATCCAAAGAATGGACACGGAGAATTTCAGGACCA129520.11768632251776766No Hit
ATTGTAAGCTGGAAAAAACACTTGAGGAGTTGCAGTACACAGTGAGATTT128590.11684129256145571No Hit
CCTTGGTCAGTGATACCAGCACCAACTGAGTAATGAATCAGCCTCAGCCC128110.11640514806787534No Hit
GGAGTTGCAGTACACAGTGAGATTTTCTCCCTCTTGGATGCTTAGAAACT127500.11585088110728364No Hit
GTGCTCACCCATGCCAACTGAATCCAAAGAATGGACACGGAGAATTTCAG126690.11511488727436678No Hit
CTGAGGGCTCTGCTCCAGCAGCTGGGTGCTCACCCATGCCAACTGAATCC125370.11391548991702077No Hit
GGTGCTCACCCATGCCAACTGAATCCAAAGAATGGACACGGAGAATTTCA124800.11339756833089411No Hit
ATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACC124230.11287964674476741No Hit
AGCAGCTGGGTGCTCACCCATGCCAACTGAATCCAAAGAATGGACACGGA124030.11269791987244228No Hit
CCTGGGTCTTGTCGATACCAGGACATGTATTCATGGTTCATATCCTGGGC123610.11231629344055946No Hit
AGCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACATGTATTCATG123250.11198918507037418No Hit
GGACCTGCCCACAGGAGAGACAAGGCTGCACAGCACAGGAGGCCGATGCT121730.11060806084070304No Hit
CCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAAT121190.11011739828542513No Hit
GGCTCCTGTCTGTACCATTGTAAGCTGGAAAAAACACTTGAGGAGTTGCA120210.1092269366110319No Hit
CTCTTGGATGCTTAGAAACTGAGGGCTCTGCTCCAGCAGCTGGGTGCTCA120110.10913607317486931No Hit
GACACGGAGAATTTCAGGACCATCTTGTCTTTCTATCACATGGTGGACAT120000.10903612339509049No Hit
GGTTGATCTGGAGACATTGTAGCCATTGGGGACTTCTCCTTGGTCAGTGA119620.1086908423376727No Hit
GTGACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGGCTGCACA118770.1079185031302908No Hit
CCTGTCTGTACCATTGTAAGCTGGAAAAAACACTTGAGGAGTTGCAGTAC118640.10780038066327946No Hit
CTGAGTGACACCAGCATTCACTGGACCTGCCCACAGGAGAGACAAGGCTG117060.10636473837191077No Hit
TGAGGAGTTGCAGTACACAGTGAGATTTTCTCCCTCTTGGATGCTTAGAA116630.10597402559641168No Hit
GAAACTGAGGGCTCTGCTCCAGCAGCTGGGTGCTCACCCATGCCAACTGA116440.1058013850677028No Hit
CTTCTCCTTGGTCAGTGATACCAGCACCAACTGAGTAATGAATCAGCCTC116030.10542884497943623No Hit
GGCTCTGCTCCAGCAGCTGGGTGCTCACCCATGCCAACTGAATCCAAAGA115650.10508356392201845No Hit
CAACTGAATCCAAAGAATGGACACGGAGAATTTCAGGACCATCTTGTCTT115340.10480188726991446No Hit
AATCAGCCTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACATGTATT115310.1047746282390657No Hit
GGAGGACAGGACCTTCCCCAGGCTCCTGTCTGTACCATTGTAAGCTGGAA115280.10474736920821692No Hit
CTGCTCCAGCAGCTGGGTGCTCACCCATGCCAACTGAATCCAAAGAATGG113940.10352979916363843No Hit
CACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGT113800.10340259035301082No Hit
GATGCTTAGAAACTGAGGGCTCTGCTCCAGCAGCTGGGTGCTCACCCATG113550.10317543176260437No Hit
CGGAGAATTTCAGGACCATCTTGTCTTTCTATCACATGGTGGACATGGCC112890.10257573308393138No Hit
ACAGGACCTTCCCCAGGCTCCTGTCTGTACCATTGTAAGCTGGAAAAAAC112470.10219410665204855No Hit
AACTGAGGGCTCTGCTCCAGCAGCTGGGTGCTCACCCATGCCAACTGAAT112440.10216684762119979No Hit
ATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCA112360.10209415687226972No Hit
ATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTG112280.10202146612333966No Hit
ACCAGGAGGACAGGACCTTCCCCAGGCTCCTGTCTGTACCATTGTAAGCT111550.10135816303935286No Hit
GCCATTGGGGACTTCTCCTTGGTCAGTGATACCAGCACCAACTGAGTAAT110230.10015876568200688No Hit
CTCAGCCCCATGCCTGGGTCTTGTCGATACCAGGACATGTATTCATGGTT110110.10004972955861179No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)