FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17851811
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTT8448934.732813942518213No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC5251162.9415278931644524No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC4327452.4240957962192184No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTTA1622880.9090842380081214No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG1121190.6280539268536957No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG1097820.614962818058067No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTTACT862960.48340193608368365No Hit
CTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACA573820.3214351754004118No Hit
GTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGAC472260.2645445887814968No Hit
GGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGG455100.2549321186517155No Hit
GGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGG437680.2451740050351194No Hit
GTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTC432180.24209308512172797No Hit
GGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGG429300.24047980342162484No Hit
GTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGC408680.2289291545826919No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG381270.21357497006886306No Hit
GTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCT375070.21010193307558545No Hit
GAAAAGGCCACACAGCACAGGAGCCCGATGCTCATGGCAGGTGCTACAGG371290.20798450084420003No Hit
GTCACAGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGAC367350.20577744185169786No Hit
CCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCAC335510.18794171638944643No Hit
GTCGATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGT326480.18288340605891468No Hit
GGAGAGAAAAGGCCACACAGCACAGGAGCCCGATGCTCATGGCAGGTGCT325940.18258091574014534No Hit
GTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGA325460.18231203545679484No Hit
GCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTTATGG324290.18165663976612792No Hit
ATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGA322070.18041306845563176No Hit
GCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAG316840.17748339370162503No Hit
TGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAG314120.1759597387626387No Hit
CACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACACAGCAC310660.174021560053487No Hit
CCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACACAGCACAG309050.17311969076974879No Hit
GTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTTATGGTTCATA307900.1724754984242215No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT307850.17244749006137247No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG304730.1706997682195941No Hit
GCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGA304450.1705429213876396No Hit
CCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCAC302500.1694505952365281No Hit
GCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGG300930.1685711326430691No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC300880.16854312428022009No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCCTGCCAG298710.16732756133257293No Hit
GCTGTTTGTACCAGAACAAATAATAATTACTCTCACTGGTGTCATATGTG297530.1666665639693362No Hit
CTTGTCGATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGC296100.16586552479185446No Hit
GTGCAACTCAGGGTCACAGTCTCTGCCTCCTGCACAGACATCTCTGGTTG295850.1657254829776094No Hit
GTCAGTGGTACCCTCAGAAGCTGAGTAATAAATCAGCCTCAGTCCCATGC295840.1657198813050396No Hit
GTCTTGTCGATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACT294580.16501407056124445No Hit
GTAATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACA294410.16491884212755784No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC289550.16219642925863376No Hit
AATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATG288080.16137298339087278No Hit
CTTGCCCACAGGAGAGAAAAGGCCACACAGCACAGGAGCCCGATGCTCAT282630.15832007184033037No Hit
GGCTGTTTGTACCAGAACAAATAATAATTACTCTCACTGGTGTCATATGT281510.15769268451251248No Hit
GGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCC280930.15736778750346392No Hit
GTTTAATCTGGAGACATTGTAGCCATTGGGGACTTCTCCTTTGTCAGTGG280590.15717733063609066No Hit
CCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGC279970.15683002693676287No Hit
CTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGAC279600.1566227650516802No Hit
ATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGA276020.15461736627169087No Hit
GCCGACTCCAGCCTGAGCGAGAACTCCCGTTTGTTTAATCTGGAGACATT274960.1540235889792918No Hit
CTCAGAAGCTGAGTAATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTC267630.1499175629856265No Hit
CTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCT266360.14920615056926156No Hit
GAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACA261940.14673021129340882No Hit
GGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAG261010.14620925574441718No Hit
CTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACT258810.14497688777906062No Hit
CACTGGACTTGCCCACAGGAGAGAAAAGGCCACACAGCACAGGAGCCCGA257660.14433269543353333No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG256380.1436156813445986No Hit
CTTCTCCTTTGTCAGTGGTACCCTCAGAAGCTGAGTAATAAATCAGCCTC256140.14348124120292333No Hit
CATATGTGCAACTCAGGGTCACAGTCTCTGCCTCCTGCACAGACATCTCT255870.14332999604353866No Hit
CCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTTATGGTTCATATC253810.14217605149415932No Hit
GGTGTCATATGTGCAACTCAGGGTCACAGTCTCTGCCTCCTGCACAGACA253660.14209202640561228No Hit
GTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTG251980.14115094541388545No Hit
GATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGTC248750.13934160517383923No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC248590.13925197841272238No Hit
GGTCACAGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGA246500.13808122884563365No Hit
CATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACAC245060.13727458799558206No Hit
GGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAA243880.13661359063234538No Hit
GGTACCCTCAGAAGCTGAGTAATAAATCAGCCTCAGTCCCATGCCTGGGT236790.13264200478035534No Hit
GTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGG236520.13249075962097068No Hit
ACGAGAATCATCTGCCTGCTGGGAGGCTGTTTGTACCAGAACAAATAATA236040.13222187933762014No Hit
CTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCA233180.1306198009826566No Hit
GCTGAGTAATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCA232180.13005963372567636No Hit
GGGTCACAGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTG229000.12827830184847913No Hit
GGACTTGCCCACAGGAGAGAAAAGGCCACACAGCACAGGAGCCCGATGCT227670.1275332793966954No Hit
ACTCAGGGTCACAGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACT227070.1271971790425072No Hit
GTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACACA226170.126693028511225No Hit
ATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACC224070.12551667727156646No Hit
TATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTC222130.12442995279302477No Hit
CATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTC221480.12406584407598759No Hit
CACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGT221100.1238529805183351No Hit
CCAGAACAAATAATAATTACTCTCACTGGTGTCATATGTGCAACTCAGGG220850.12371293870409002No Hit
AATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGT219380.12288949283632905No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAGA215620.12078326395008328No Hit
AGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTC210010.11764072563842402No Hit
ACCAGAACAAATAATAATTACTCTCACTGGTGTCATATGTGCAACTCAGG207530.11625151084111299No Hit
CCTGGGTCTTGTCGATACCAGTACATGGAGTTATGGTTCATATCCTGGGC207410.11618429077027537No Hit
GCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGACTGTCTGGGCCAT207130.11602744393832089No Hit
GTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGACTGTCTG206680.11577536867267976No Hit
GGGGACTTCTCCTTTGTCAGTGGTACCCTCAGAAGCTGAGTAATAAATCA206650.11575856365497036No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA204990.11482868600838313No Hit
GGCCACACAGCACAGGAGCCCGATGCTCATGGCAGGTGCTACAGGATGGA204830.11473905924726628No Hit
CTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGC203860.11419569700799542No Hit
CCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTTATGGT202410.11338345448537407No Hit
CTCACTGGTGTCATATGTGCAACTCAGGGTCACAGTCTCTGCCTCCTGCA199700.11186540121895756No Hit
GCCCGATGCTCATGGCAGGTGCTACAGGATGGAGGGGTCTTCTGGGTCTG197750.11077307506784605No Hit
GGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTG196300.1099608325452247No Hit
GTCATATGTGCAACTCAGGGTCACAGTCTCTGCCTCCTGCACAGACATCT195410.10946228368651226No Hit
CCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAAT195260.10937825859796521No Hit
ATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTT195070.10927182681913895No Hit
AGAACAAATAATAATTACTCTCACTGGTGTCATATGTGCAACTCAGGGTC194880.10916539504031272No Hit
ATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCA193160.10820190735830668No Hit
ACTGGACTTGCCCACAGGAGAGAAAAGGCCACACAGCACAGGAGCCCGAT192390.10777057857043186No Hit
GCCATTGGGGACTTCTCCTTTGTCAGTGGTACCCTCAGAAGCTGAGTAAT191390.10721041131345162No Hit
CCTTTGTCAGTGGTACCCTCAGAAGCTGAGTAATAAATCAGCCTCAGTCC191020.10700314942836892No Hit
GCGAGAACTCCCGTTTGTTTAATCTGGAGACATTGTAGCCATTGGGGACT190930.1069527343752407No Hit
GCAACTCAGGGTCACAGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAG190690.10681829423356544No Hit
GCCCACAGGAGAGAAAAGGCCACACAGCACAGGAGCCCGATGCTCATGGC189260.10601725505608366No Hit
GACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACACAGC188880.10580439149843117No Hit
GTTTGTACCAGAACAAATAATAATTACTCTCACTGGTGTCATATGTGCAA187250.10489131886955334No Hit
GAGTAATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTA185930.10415189809033941No Hit
CGATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGT185840.10410148303721119No Hit
CCCACAGGAGAGAAAAGGCCACACAGCACAGGAGCCCGATGCTCATGGCA184620.10341807898369527No Hit
CTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATT183220.1026338448239229No Hit
GTTGCTTATAAGCTTCTTGGCGAATAACGAGAATCATCTGCCTGCTGGGA182250.10209048258465205No Hit
GCCTGCTGGGAGGCTGTTTGTACCAGAACAAATAATAATTACTCTCACTG182060.10198405080582582No Hit
GAGAAAAGGCCACACAGCACAGGAGCCCGATGCTCATGGCAGGTGCTACA179640.10062844604393359No Hit
AGCTTCTTGGCGAATAACGAGAATCATCTGCCTGCTGGGAGGCTGTTTGT179410.10049960757482813No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTT179090.10032035405259443No Hit
GCTGCTTTCTGGAAGTTCACAGAGAAACGATTCTCCGTTGCATTCTGTTG179020.10028114234460583No Hit
GACATCTCTGGTTGAGACTGAGTGACTGTCTGGGCCATGCCGGATTCAAG178980.10025873565432661No Hit
GCACAGACATCTCTGGTTGAGACTGAGTGACTGTCTGGGCCATGCCGGAT178590.10004027042410432No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)