FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18793653
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC8452294.497417292955233No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC7114883.7857887447427068No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG2378831.26576243586066No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1687590.8979574114729053No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1428330.7600065830735515No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT498580.2652916918280868No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC483700.25737412519003094No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGA474620.252542706838314No Hit
GCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTT452090.2405546170294833No Hit
CCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCA437780.23294034427473997No Hit
GCCTTGGAGCCCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCGG384860.20478190163455715No Hit
GTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAA369840.19678984176200337No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTT360070.19159127818311852No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG357970.19047387966565094No Hit
GACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCG353860.1882869711386073No Hit
CCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTA335970.1787677999588478No Hit
GGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACG305810.16271982886988495No Hit
GGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCT304590.16207067354068952No Hit
CTCTGTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTC300530.1599103697402522No Hit
CCCAGCTCGGATGTTGAGAGACGACAGCACCAAGCCCGGAGCCTGGCCCC298720.1589472786371016No Hit
GGAAGTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGACGATACCAAA298580.15887278540260374No Hit
GCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGAT292070.15540884999845425No Hit
GTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGG283340.15076366473298192No Hit
GCCATCAGCATGAGACTCTGTTTCGGGAACTGACGATACCAAAACATAGT279280.14860336093254461No Hit
GGCTTGCATGGTTGATGAGAAACTTGTCCTTCTCGACGCCTTGCTCGTAT278520.1481989690881278No Hit
GTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCC271600.14451687492580606No Hit
GCATGAGACTCTGTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCC265180.14110082802954807No Hit
GTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGACGATACCAAAACAT264760.14087734832605453No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGATG254390.13535952802789325No Hit
CGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGG254010.1351573321056848No Hit
GGTTCCACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGACAGCAC252710.1344656092139192No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG244640.13017160633965094No Hit
CAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCC236850.12602658993437837No Hit
GTTCCACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGACAGCACC231030.12292979975739682No Hit
CTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACT224030.11920513803250492No Hit
ACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGATGT222910.11860919215652221No Hit
GGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGG219300.11668833089554223No Hit
CTCGGATGTTGAGAGACGACAGCACCAAGCCCGGAGCCTGGCCCCAGAAG217370.11566138844853631No Hit
GTGGCCTTGGAGCCCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTT212610.11312861847560983No Hit
GACTCTGTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAG212160.1128891759361525No Hit
GACGACAGCACCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGAAGCAGCA211810.1127029428499079No Hit
GATAACCCAGCTCGGATGTTGAGAGACGACAGCACCAAGCCCGGAGCCTG211710.11264973339669515No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC210970.11225598344292087No Hit
CACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGACAGCACCAAGC210360.11193140577832315No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTGTG208880.11114390587077455No Hit
GCCCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGAC206810.1100424701892708No Hit
CTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGATGTT206590.10992540939220277No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC202700.10785556166222714No Hit
GAAACTTGTCCTTCTCGACGCCTTGCTCGTATGTGGCCTTGGAGCCCTCA201230.10707338269999983No Hit
GCACCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGAAGCAGCAGCATCTT200130.10648807871465968No Hit
CCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACC199750.10628588279245127No Hit
CTTACAGATAACCCAGCTCGGATGTTGAGAGACGACAGCACCAAGCCCGG197790.10524297750948153No Hit
GGACAAGGTCAGGCTTGCATGGTTGATGAGAAACTTGTCCTTCTCGACGC197290.10497693024341782No Hit
GGTCAGGCTTGCATGGTTGATGAGAAACTTGTCCTTCTCGACGCCTTGCT196530.10457253839900099No Hit
AGTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGACGATACCAAAACA196380.10449272421918186No Hit
CTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTAC195700.10413089993733522No Hit
GGCACTGGTCACTGTCAGAGTGGACAAGGTCAGGCTTGCATGGTTGATGA195350.10394466685109062No Hit
AGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATA194020.10323698112336117No Hit
CGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGAT193410.10291240345876344No Hit
GGTCACTGTCAGAGTGGACAAGGTCAGGCTTGCATGGTTGATGAGAAACT193050.10272084942719757No Hit
CCTTGGAGCCCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCGGG192740.10255590012223809No Hit
GAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCT190180.1011937381199919No Hit
ACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGAC188250.10016679567298598No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)