FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11121458
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC6367025.724986777812765No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC6068795.456829491241166No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1134171.0198033387349033No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1080300.9713654450702417No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG1056650.9501002476473859No Hit
CTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACA414510.372711923202875No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC399420.3591435583356067No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT380030.34170879393691006No Hit
GGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGG327290.29428695410260053No Hit
GTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGAC318330.28623045647432194No Hit
GGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGG292550.26305004253938646No Hit
GTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTC291000.2616563403827088No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA280900.25257479729726084No Hit
GGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGG276210.24835772431995876No Hit
GAAAAGGCCACACAGCACAGGAGCCCGATGCTCATGGCAGGTGCTACAGG270930.24361014536043746No Hit
GTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGC255250.2295112745109499No Hit
GGAGAGAAAAGGCCACACAGCACAGGAGCCCGATGCTCATGGCAGGTGCT246860.221967299611256No Hit
GTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCT246750.221868391716266No Hit
CCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCAC233930.21034112613651915No Hit
GTCGATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGT227100.20419984502032018No Hit
GTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGA224220.20161025649694495No Hit
ATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGA220680.1984272206036295No Hit
GCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTTATGG219970.19778881509960297No Hit
GCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAG219270.19715940122239367No Hit
GTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTTATGGTTCATA217090.19519922657622768No Hit
AATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATG216360.19454283781856657No Hit
TGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAG215020.1933379598250517No Hit
CACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACACAGCAC212110.19072139642122463No Hit
GCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGA209650.18850945622417492No Hit
ATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGA208410.1873944944988328No Hit
GTAATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACA206100.18531742870404222No Hit
GTCAGTGGTACCCTCAGAAGCTGAGTAATAAATCAGCCTCAGTCCCATGC204660.1840226344423546No Hit
CCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACACAGCACAG203160.1826738904197633No Hit
CTTGTCGATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGC203030.18255699927113872No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC198480.1784658090692785No Hit
CCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCAC196770.17692824088352443No Hit
CCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGC194590.17496806623735844No Hit
GCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGG194330.1747342839401093No Hit
GTTTAATCTGGAGACATTGTAGCCATTGGGGACTTCTCCTTTGTCAGTGG193440.1739340291533718No Hit
GGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCC192540.17312478273981705No Hit
GCCGACTCCAGCCTGAGCGAGAACTCCCGTTTGTTTAATCTGGAGACATT190600.17138040713726566No Hit
GTCTTGTCGATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACT189780.17064309373824907No Hit
CACTGGACTTGCCCACAGGAGAGAAAAGGCCACACAGCACAGGAGCCCGA188430.16942922411791692No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG188180.16920443344748504No Hit
CTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGAC184240.16566173248147859No Hit
GAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACA184090.16552685807921946No Hit
CTCAGAAGCTGAGTAATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTC183550.1650413102310866No Hit
CTTGCCCACAGGAGAGAAAAGGCCACACAGCACAGGAGCCCGATGCTCAT183250.16477156142656835No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATGA182620.16420508893708No Hit
CTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCT180210.16203810687411668No Hit
GGTACCCTCAGAAGCTGAGTAATAAATCAGCCTCAGTCCCATGCCTGGGT177520.15961935926026966No Hit
CCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTTATGGTTCATATC177180.1593136439484823No Hit
GTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTG173700.15618455781607052No Hit
GATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGTC172490.1550965709711802No Hit
CTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACT171350.15407152551401085No Hit
GGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAG169400.15231815828464218No Hit
GTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGG166380.14960268698582507No Hit
AATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGT165250.14858663315547296No Hit
CATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACAC164290.14772343698101453No Hit
GGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAA161570.14527771448671567No Hit
GGACTTGCCCACAGGAGAGAAAAGGCCACACAGCACAGGAGCCCGATGCT160370.14419871926864267No Hit
CTTCTCCTTTGTCAGTGGTACCCTCAGAAGCTGAGTAATAAATCAGCCTC158370.1424003939051876No Hit
AGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTC158180.1422295529956594No Hit
CATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTC157060.14122249079212457No Hit
GTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACACA155250.13959500633819774No Hit
GGGGACTTCTCCTTTGTCAGTGGTACCCTCAGAAGCTGAGTAATAAATCA154720.13911845011688218No Hit
ATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACC154390.13882172643191207No Hit
CTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCA154370.13880374317827754No Hit
TATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTC152520.13714029221708163No Hit
GCCCGATGCTCATGGCAGGTGCTACAGGATGGAGGGGTCTTCTGGGTCTG148440.13347170847563333No Hit
GCTGAGTAATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCA148200.1332559094320187No Hit
CACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGT146840.1320330481848693No Hit
CCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTTATGGT146080.13134968454675638No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA146030.13130472641267No Hit
GGCCACACAGCACAGGAGCCCGATGCTCATGGCAGGTGCTACAGGATGGA145890.13117884363722812No Hit
ATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTT143320.1288679955451884No Hit
CCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAAT143300.12885001229155385No Hit
CCTGGGTCTTGTCGATACCAGTACATGGAGTTATGGTTCATATCCTGGGC142180.12784295008801905No Hit
ATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCA140500.1263323567827168No Hit
GGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTG137300.12345503620118874No Hit
GACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACACAGC135200.12156679456956095No Hit
GAGTAATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTA135100.12147687830138817No Hit
ACTGGACTTGCCCACAGGAGAGAAAAGGCCACACAGCACAGGAGCCCGAT134430.12087443930463074No Hit
CGATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGT134160.12063166538056432No Hit
CCTTTGTCAGTGGTACCCTCAGAAGCTGAGTAATAAATCAGCCTCAGTCC133830.12033494169559422No Hit
CTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATT133750.12026300868105604No Hit
AAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAG132830.1194357790138667No Hit
GCCATTGGGGACTTCTCCTTTGTCAGTGGTACCCTCAGAAGCTGAGTAAT130930.11772736991858442No Hit
GCGAGAACTCCCGTTTGTTTAATCTGGAGACATTGTAGCCATTGGGGACT130440.11728678020453794No Hit
ACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGT130010.1169001402513951No Hit
CTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGC129580.11651350029825225No Hit
GCCCACAGGAGAGAAAAGGCCACACAGCACAGGAGCCCGATGCTCATGGC129080.1160639189573885No Hit
GGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTT128880.11588408642104299No Hit
GAGAAAAGGCCACACAGCACAGGAGCCCGATGCTCATGGCAGGTGCTACA126780.1139958447894152No Hit
TGTCGATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAG125020.11241331846957477No Hit
AAAGGCCACACAGCACAGGAGCCCGATGCTCATGGCAGGTGCTACAGGAT124430.11188281248735552No Hit
GCACAGGAGCCCGATGCTCATGGCAGGTGCTACAGGATGGAGGGGTCTTC122730.11035423592841874No Hit
GCCTGAGCGAGAACTCCCGTTTGTTTAATCTGGAGACATTGTAGCCATTG122200.10987767970710315No Hit
CCCACAGGAGAGAAAAGGCCACACAGCACAGGAGCCCGATGCTCATGGCA121700.10942809836623939No Hit
GGAGCCCGATGCTCATGGCAGGTGCTACAGGATGGAGGGGTCTTCTGGGT120910.10871775984767464No Hit
GTGGTACCCTCAGAAGCTGAGTAATAAATCAGCCTCAGTCCCATGCCTGG120200.10807935434364811No Hit
AGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTTATG119680.1076117897491498No Hit
AGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACAC119190.1071712000351033No Hit
CTCCTTTGTCAGTGGTACCCTCAGAAGCTGAGTAATAAATCAGCCTCAGT119100.10709027539374782No Hit
AATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAG118280.10635296199473125No Hit
ATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGG116690.10492329333078451No Hit
CTCCCGTTTGTTTAATCTGGAGACATTGTAGCCATTGGGGACTTCTCCTT116660.10489631845033269No Hit
TGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCT116510.10476144404807355No Hit
CCCGTTTGTTTAATCTGGAGACATTGTAGCCATTGGGGACTTCTCCTTTG116410.1046715277799008No Hit
GAAGCTGAGTAATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATA115390.10375438184453874No Hit
ATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTG114820.10324185911595404No Hit
AGTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTC112830.10145252537931627No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATGACT112320.10099395241163524No Hit
GGAGACATTGTAGCCATTGGGGACTTCTCCTTTGTCAGTGGTACCCTCAG111410.10017571437126319No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)