FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9381205
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC5695116.070765962368374No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC4824665.14290008586317No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC4464104.7585571363167105No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC3672783.915040765018993No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG3329743.5493734546894564No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGA711210.7581222241705623No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG493080.5256041201530081No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG491510.5239305611592541No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGATA444930.47427809114074365No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT371060.39553554154290416No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC363600.38758347141971633No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC325680.34716222489541587No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGAAAAG304720.32481967934822875No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT302370.3223146706633103No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCCTGCCAG290690.30986424451869454No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCCAAAGGG249510.2659679646697839No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC242620.2586234923978316No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG239970.2557986953701577No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG208160.2218904714266451No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA196130.20906695888214788No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGCTGCG185270.19749062087439728No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG178200.18995427559679168No Hit
GTCAGGAAGGCCTCTTCTGAGAGAGTCACTGGCCCTTCCATCTGGGTCAC170540.18178901324510016No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTT147600.15733586463572644No Hit
GGAAGCACTGCATCTGATTCAGGGCTTGGTTCTCCTGGGGAAGAGCCGAG142130.1515050571861504No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG139950.14918126189546013No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA128730.13722117787640287No Hit
GTTTATAGTCAGGAAGGCCTCTTCTGAGAGAGTCACTGGCCCTTCCATCT125570.1338527406660445No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC122860.13096398596981945No Hit
GGAGGAGCTGTAGACCTTCTCCAGGATATTGGACATACCAGAAAAGGGAA120810.12877876562765658No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCAAGTCC112240.11964347863627327No Hit
GCCCGGGGCCCATGGCACAGAGGGACAGGAAGCACTGCATCTGATTCAGG109250.11645625482014305No Hit
GCCCAGCAGAGGAGCCCGGGGCCCATGGCACAGAGGGACAGGAAGCACTG107810.11492127077491643No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG105330.11227768714147063No Hit
GGGAAGGGTATCCTGTGGCTGTGTACGTGCAGTTTATAGTCAGGAAGGCC104190.11106249143899957No Hit
CCAGGATATTGGACATACCAGAAAAGGGAAGGGTATCCTGTGGCTGTGTA103560.11039093591921292No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAGA102990.10978333806797741No Hit
GGACATACCAGAAAAGGGAAGGGTATCCTGTGGCTGTGTACGTGCAGTTT100630.10726766977163381No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGA99240.10578598378353314No Hit
GCCTCTTCTGAGAGAGTCACTGGCCCTTCCATCTGGGTCACTGAATTTCC95770.10208709861899404No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)