FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19574774
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT11920806.089878738829884No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC2852441.4572020090755582No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC2353721.2024251212300074No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTTA2351271.2011735103557262No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1719040.8781914927855617No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1440470.7358807820718645No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTTACT1270110.6488504030748963No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG766970.39181550703982587No Hit
GTCACGGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGAC761130.3888320754048042No Hit
GCTCAGGGTCACGGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACT667410.3409541280016822No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG575150.2938220385073156No Hit
GGCTGCTTGTACCAGAATAAATAATAATCACTCTCACTGGTGTCATATGT456560.23323896357628446No Hit
CATATGTGCAGCTCAGGGTCACGGTCTCTGCCTCCTGCACAGACATCTCT443950.22679699903559553No Hit
GGTGTCATATGTGCAGCTCAGGGTCACGGTCTCTGCCTCCTGCACAGACA440140.22485061641069268No Hit
GCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGACTGTCTGAGCCAT432510.22095274254507355No Hit
GGGTCACGGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTG420030.21457719000995873No Hit
GTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGACTGTCTG409890.20939705357517793No Hit
GTCATATGTGCAGCTCAGGGTCACGGTCTCTGCCTCCTGCACAGACATCT405160.20698067829544292No Hit
GCTGCTTGTACCAGAATAAATAATAATCACTCTCACTGGTGTCATATGTG405100.20695002660056253No Hit
ACGAGAATCATCTGCCTGCTGGGAGGCTGCTTGTACCAGAATAAATAATA402990.20587210866393657No Hit
GACATCTCTGGTTGAGACTGAGTGACTGTCTGAGCCATGCTAAATTCAAG395210.20189760556111658No Hit
CCAGAATAAATAATAATCACTCTCACTGGTGTCATATGTGCAGCTCAGGG380590.19442880924193556No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCCTGCCAG379340.1937902322652614No Hit
GGTTGAGACTGAGTGACTGTCTGAGCCATGCTAAATTCAAGACAGGTGGA376290.19223210444217645No Hit
GCTAAATTCAAGACAGGTGGAGATCACAAGTGCCCACAGGAAGCCAGGGC374050.19108777449997635No Hit
GACTGAGTGACTGTCTGAGCCATGCTAAATTCAAGACAGGTGGAGATCAC367150.18756282958873496No Hit
GTGCAGCTCAGGGTCACGGTCTCTGCCTCCTGCACAGACATCTCTGGTTG364850.18638784795165453No Hit
GTTGAGACTGAGTGACTGTCTGAGCCATGCTAAATTCAAGACAGGTGGAG363530.1857135106642866No Hit
GCACAGACATCTCTGGTTGAGACTGAGTGACTGTCTGAGCCATGCTAAAT354200.18094717211039063No Hit
CTCTGGTTGAGACTGAGTGACTGTCTGAGCCATGCTAAATTCAAGACAGG334200.17072994048360407No Hit
GGTCACGGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGA329320.1682369359666681No Hit
GTTGCATTCTGTTGCTTATAAGCTTCTTGGCGAATAACGAGAATCATCTG320790.16387928667784363No Hit
GTCTGAGCCATGCTAAATTCAAGACAGGTGGAGATCACAAGTGCCCACAG319790.16336842509650432No Hit
CACGGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGACTG314920.16088052919538176No Hit
GGTGGAGATCACAAGTGCCCACAGGAAGCCAGGGCATGCCATGCTCACAG314680.16075792241586034No Hit
AGAATAAATAATAATCACTCTCACTGGTGTCATATGTGCAGCTCAGGGTC314170.16049738300937727No Hit
ACCAGAATAAATAATAATCACTCTCACTGGTGTCATATGTGCAGCTCAGG312370.15957783216296648No Hit
GCATTCTGTTGCTTATAAGCTTCTTGGCGAATAACGAGAATCATCTGCCT304000.1553019207271563No Hit
CTGCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGACTGTCTGAGCC302250.15440791295981246No Hit
GTTGCTTATAAGCTTCTTGGCGAATAACGAGAATCATCTGCCTGCTGGGA301680.15411672185844905No Hit
GCCTGCTGGGAGGCTGCTTGTACCAGAATAAATAATAATCACTCTCACTG300250.1533861897971338No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA291050.14868626324881196No Hit
AGCTTCTTGGCGAATAACGAGAATCATCTGCCTGCTGGGAGGCTGCTTGT290750.14853300477441017No Hit
CAGACATCTCTGGTTGAGACTGAGTGACTGTCTGAGCCATGCTAAATTCA286740.14648444983323947No Hit
CTGAGTGACTGTCTGAGCCATGCTAAATTCAAGACAGGTGGAGATCACAA284180.14517664418501078No Hit
GCCATGCTAAATTCAAGACAGGTGGAGATCACAAGTGCCCACAGGAAGCC282720.14443078627625533No Hit
GCAGCTCAGGGTCACGGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAG282440.14428774503348032No Hit
GCTGCTTTCTGGAAGTTCACAGAGAAACGATTCTCTGTTGCATTCTGTTG281310.1437104714465669No Hit
GGCGAATAACGAGAATCATCTGCCTGCTGGGAGGCTGCTTGTACCAGAAT279450.14276026890527574No Hit
AGACTGAGTGACTGTCTGAGCCATGCTAAATTCAAGACAGGTGGAGATCA276510.14125833585613812No Hit
GGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGACTGTCT272580.13925064984147453No Hit
CGGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGACTGTC269010.13742687399609313No Hit
GAAGGATTTGGCTGCTTTCTGGAAGTTCACAGAGAAACGATTCTCTGTTG263780.13475506792568842No Hit
GGGAGGCTGCTTGTACCAGAATAAATAATAATCACTCTCACTGGTGTCAT263120.13441789928200448No Hit
GGAAGTTCACAGAGAAACGATTCTCTGTTGCATTCTGTTGCTTATAAGCT262300.13399899278530625No Hit
ACGGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGACTGT259920.13278314222171864No Hit
CTCAGGGTCACGGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGACTG258680.13214967386085785No Hit
CTGGTGTCATATGTGCAGCTCAGGGTCACGGTCTCTGCCTCCTGCACAGA253130.12931439208442458No Hit
CGAATAACGAGAATCATCTGCCTGCTGGGAGGCTGCTTGTACCAGAATAA252380.1289312458984201No Hit
CGAGAATCATCTGCCTGCTGGGAGGCTGCTTGTACCAGAATAAATAATAA249170.12729138022232084No Hit
AATCACTCTCACTGGTGTCATATGTGCAGCTCAGGGTCACGGTCTCTGCC248000.12669367217215383No Hit
GCTTATAAGCTTCTTGGCGAATAACGAGAATCATCTGCCTGCTGGGAGGC244430.1248698963267724No Hit
GAGGCTGCTTGTACCAGAATAAATAATAATCACTCTCACTGGTGTCATAT242270.12376643531107946No Hit
ATTCAAGACAGGTGGAGATCACAAGTGCCCACAGGAAGCCAGGGCATGCC241940.12359785098923748No Hit
CTCACTGGTGTCATATGTGCAGCTCAGGGTCACGGTCTCTGCCTCCTGCA241770.12351100452040978No Hit
CTCTGCCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGACTGTCTGAG241650.12344970113064907No Hit
ATCACTCTCACTGGTGTCATATGTGCAGCTCAGGGTCACGGTCTCTGCCT241380.12331176850368746No Hit
GACTGTCTGAGCCATGCTAAATTCAAGACAGGTGGAGATCACAAGTGCCC241050.12314318418184546No Hit
GCTTGTACCAGAATAAATAATAATCACTCTCACTGGTGTCATATGTGCAG240780.12300525155488386No Hit
AAATAATAATCACTCTCACTGGTGTCATATGTGCAGCTCAGGGTCACGGT240710.1229694912441901No Hit
CTCTCACTGGTGTCATATGTGCAGCTCAGGGTCACGGTCTCTGCCTCCTG239870.12254036751586507No Hit
CACTGGTGTCATATGTGCAGCTCAGGGTCACGGTCTCTGCCTCCTGCACA238180.1216770114434016No Hit
GAATCATCTGCCTGCTGGGAGGCTGCTTGTACCAGAATAAATAATAATCA233000.11903074845206388No Hit
AATAATAATCACTCTCACTGGTGTCATATGTGCAGCTCAGGGTCACGGTC227580.1162618786812047No Hit
AACGAGAATCATCTGCCTGCTGGGAGGCTGCTTGTACCAGAATAAATAAT227000.11596557896402789No Hit
CTCCTGCACAGACATCTCTGGTTGAGACTGAGTGACTGTCTGAGCCATGC225870.11538830537711445No Hit
GAGTGACTGTCTGAGCCATGCTAAATTCAAGACAGGTGGAGATCACAAGT225020.11495407303297601No Hit
CATCTCTGGTTGAGACTGAGTGACTGTCTGAGCCATGCTAAATTCAAGAC224870.11487744379577512No Hit
GTACCAGAATAAATAATAATCACTCTCACTGGTGTCATATGTGCAGCTCA221290.11304855933458031No Hit
GTGACTGTCTGAGCCATGCTAAATTCAAGACAGGTGGAGATCACAAGTGC221100.11295149563412585No Hit
ATCATCTGCCTGCTGGGAGGCTGCTTGTACCAGAATAAATAATAATCACT216900.11080587699250065No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC216590.11064750990228546No Hit
ATCTGCCTGCTGGGAGGCTGCTTGTACCAGAATAAATAATAATCACTCTC214350.10950317996008536No Hit
GAGAAACGATTCTCTGTTGCATTCTGTTGCTTATAAGCTTCTTGGCGAAT214310.10948274549683179No Hit
GCGAATAACGAGAATCATCTGCCTGCTGGGAGGCTGCTTGTACCAGAATA213760.10920177162709517No Hit
GGAGGCTGCTTGTACCAGAATAAATAATAATCACTCTCACTGGTGTCATA210880.10773049027283789No Hit
AACGATTCTCTGTTGCATTCTGTTGCTTATAAGCTTCTTGGCGAATAACG210330.10744951640310124No Hit
CTGCTTTCTGGAAGTTCACAGAGAAACGATTCTCTGTTGCATTCTGTTGC208840.10668833264690565No Hit
CAGCTCAGGGTCACGGTCTCTGCCTCCTGCACAGACATCTCTGGTTGAGA207050.10577389041630826No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG206340.10541117869355733No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG205200.10482879649083049No Hit
GAAACGATTCTCTGTTGCATTCTGTTGCTTATAAGCTTCTTGGCGAATAA205020.10473684140618941No Hit
CACAGACATCTCTGGTTGAGACTGAGTGACTGTCTGAGCCATGCTAAATT204370.10440478137831886No Hit
CCTCCTGCACAGACATCTCTGGTTGAGACTGAGTGACTGTCTGAGCCATG203540.10398076626580721No Hit
TCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTTAC202820.10361294592724289No Hit
CTTGTACCAGAATAAATAATAATCACTCTCACTGGTGTCATATGTGCAGC200630.10249415906410976No Hit
ATAATAATCACTCTCACTGGTGTCATATGTGCAGCTCAGGGTCACGGTCT200170.10225916273669367No Hit
GTCTGAGATCTTGAGACTGAAGGATTTGGCTGCTTTCTGGAAGTTCACAG199610.10197308025114364No Hit
ATGTGCAGCTCAGGGTCACGGTCTCTGCCTCCTGCACAGACATCTCTGGT196710.1004915816652596No Hit
TACCAGAATAAATAATAATCACTCTCACTGGTGTCATATGTGCAGCTCAG196270.10026680256947028No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)