FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13492129
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC8327086.171805798773492No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC7621265.648671162275427No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA2895482.146051227348923No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTC793890.5884097313329868No Hit
CCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCA639350.4738688756978235No Hit
GCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTT609800.45196721733093426No Hit
GCCTTGGAGCCCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCGG541010.4009819354677086No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT535750.3970833661611151No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAAA532760.394867259273907No Hit
GTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAA513900.3808887389084406No Hit
GACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCG513700.38074050433404544No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC505780.3748704151879959No Hit
GGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCT446920.3312449799434915No Hit
GGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACG444610.3295328706092271No Hit
CTCTGTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTC441020.3268720599988334No Hit
GGAAGTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGACGATACCAAA434710.32219525917666514No Hit
CCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTA431690.31995691710329777No Hit
GCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGAT405330.3004196001980117No Hit
CCCAGCTCGGATGTTGAGAGACGACAGCACCAAGCCCGGAGCCTGGCCCC402700.298470315544715No Hit
GTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGG399640.2962023265564686No Hit
GCCATCAGCATGAGACTCTGTTTCGGGAACTGACGATACCAAAACATAGT392270.2907398824900058No Hit
GTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCC385400.28564802485953106No Hit
GTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGACGATACCAAAACAT378660.2806525197024132No Hit
GCATGAGACTCTGTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCC378400.2804598147556994No Hit
GGCTTGCATGGTTGATGAGAAACTTGTCCTTCTCGACGCCTTGCTCGTAT378160.2802819332664252No Hit
GGTTCCACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGACAGCAC350320.25964768051061476No Hit
CTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACT330560.24500210456036997No Hit
GGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGG323640.2398731882862964No Hit
CGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGG322150.23876884070705226No Hit
GTTCCACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGACAGCACC320220.23733837706413863No Hit
GACTCTGTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAG315160.23358804233194036No Hit
GTGGCCTTGGAGCCCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTT311370.2307789971471515No Hit
CAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCC309830.22963759092430852No Hit
ACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGATGT301720.2236266789325836No Hit
GATAACCCAGCTCGGATGTTGAGAGACGACAGCACCAAGCCCGGAGCCTG297910.22080281029035523No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAAACC297250.22031363619485106No Hit
CACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGACAGCACCAAGC294710.2184310571000322No Hit
GCCCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGAC287100.21279073154429518No Hit
GACGACAGCACCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGAAGCAGCA275260.20401524474009994No Hit
GCACCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGAAGCAGCAGCATCTT275210.20397818609650115No Hit
GAAACTTGTCCTTCTCGACGCCTTGCTCGTATGTGGCCTTGGAGCCCTCA273960.20305172000653124No Hit
CTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGATGTT269920.2000573816037484No Hit
AAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACA268680.19913832724249822No Hit
CGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGAT268020.1986491531469941No Hit
CTCGGATGTTGAGAGACGACAGCACCAAGCCCGGAGCCTGGCCCCAGAAG265190.19655163391930214No Hit
CCTTGGAGCCCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCGGG265080.1964701049033848No Hit
CCATCAGCATGAGACTCTGTTTCGGGAACTGACGATACCAAAACATAGTT262910.19486175977119696No Hit
CCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACC261090.19351282514420073No Hit
GTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACT261060.19349058995804147No Hit
GGACAAGGTCAGGCTTGCATGGTTGATGAGAAACTTGTCCTTCTCGACGC260080.19276424054350505No Hit
CTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTAC259620.1924233010223961No Hit
GGTCAGGCTTGCATGGTTGATGAGAAACTTGTCCTTCTCGACGCCTTGCT259450.19229730163416017No Hit
GAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCT257960.19119295405491601No Hit
CATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGG257000.19048142809781912No Hit
CAGAGGTTCCACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGACA256810.19034060525214366No Hit
CCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGACGA252110.18685709275385673No Hit
ACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGAC251580.18646427113170946No Hit
GGTCACTGTCAGAGTGGACAAGGTCAGGCTTGCATGGTTGATGAGAAACT251220.1861974488977981No Hit
AGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATA250740.18584168591924966No Hit
GGCCTTGGAGCCCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCG248750.18436675190401752No Hit
GGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAG248700.1843296932604187No Hit
CTTACAGATAACCCAGCTCGGATGTTGAGAGACGACAGCACCAAGCCCGG248120.18389981299467267No Hit
GGCACTGGTCACTGTCAGAGTGGACAAGGTCAGGCTTGCATGGTTGATGA247540.18346993272892662No Hit
GAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAG244090.1809128863206096No Hit
CGGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAAC243450.18043853568254498No Hit
CGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGATGTTGA236470.17526514903615287No Hit
GAGAGACGACAGCACCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGAAGC236230.17508726754687862No Hit
ATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGACGATACC235540.1745758582652152No Hit
CACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGA235120.17426456565898532No Hit
CTTCTCGACGCCTTGCTCGTATGTGGCCTTGGAGCCCTCATTGGAAGTTG225700.1672827172049719No Hit
GATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGATGTTGAG225550.1671715412741755No Hit
CTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGACGAT224840.16664530853507256No Hit
GGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGA224070.16607460542365107No Hit
ACTCTGTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGT223070.16533343255167512No Hit
GTCAGAGTGGACAAGGTCAGGCTTGCATGGTTGATGAGAAACTTGTCCTT222860.16517778624856017No Hit
CATCAGCATGAGACTCTGTTTCGGGAACTGACGATACCAAAACATAGTTG222740.16508884550392305No Hit
ATCAGCATGAGACTCTGTTTCGGGAACTGACGATACCAAAACATAGTTGT222160.164658965238177No Hit
CCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGAAGCAGCAGCATCTTCCG220620.16351755901533405No Hit
CCTTGCTCGTATGTGGCCTTGGAGCCCTCATTGGAAGTTGCCATCAGCAT219360.16258368119664435No Hit
CGACGCCTTGCTCGTATGTGGCCTTGGAGCCCTCATTGGAAGTTGCCATC218490.16193886079802527No Hit
GGATGGGCACTGGTCACTGTCAGAGTGGACAAGGTCAGGCTTGCATGGTT214040.1586406415177323No Hit
CTCGTATGTGGCCTTGGAGCCCTCATTGGAAGTTGCCATCAGCATGAGAC212260.1573213538056151No Hit
GTTGAGAGACGACAGCACCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGA211810.15698782601322592No Hit
GCCTGGCCCCAGAAGCAGCAGAAGCAGCAGCATCTTCCGTGATGGCCTCA211800.15698041428450618No Hit
ATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGATGTTGAGA210520.15603171300837695No Hit
CAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCAC210140.1557500673170261No Hit
ATGAGACTCTGTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCCTG209920.1555870092851914No Hit
GAGAAACTTGTCCTTCTCGACGCCTTGCTCGTATGTGGCCTTGGAGCCCT209870.15554995064159258No Hit
CAGATAACCCAGCTCGGATGTTGAGAGACGACAGCACCAAGCCCGGAGCC206730.15322266782358812No Hit
GTTGATGAGAAACTTGTCCTTCTCGACGCCTTGCTCGTATGTGGCCTTGG206260.1528743165737594No Hit
GAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGC205890.1526000826111283No Hit
ACCCAGCTCGGATGTTGAGAGACGACAGCACCAAGCCCGGAGCCTGGCCC205110.15202196777098706No Hit
CTTGCATGGTTGATGAGAAACTTGTCCTTCTCGACGCCTTGCTCGTATGT203620.1509176201917429No Hit
AGACTCTGTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAA199440.14781951758688344No Hit
GAAGTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGACGATACCAAAA198770.14732293176265956No Hit
ATGAGAAACTTGTCCTTCTCGACGCCTTGCTCGTATGTGGCCTTGGAGCC198630.14721916756058293No Hit
ACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCAC198390.1470412860713087No Hit
GCCCGGAGCCTGGCCCCAGAAGCAGCAGAAGCAGCAGCATCTTCCGTGAT197420.14632234838549202No Hit
GCACTGGTCACTGTCAGAGTGGACAAGGTCAGGCTTGCATGGTTGATGAG192150.14241636735017876No Hit
GTCAGGCTTGCATGGTTGATGAGAAACTTGTCCTTCTCGACGCCTTGCTC189770.140652375914876No Hit
CTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGATGTTGAGAGA189130.1401780252768114No Hit
TGAGAGACGACAGCACCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGAAG189030.14010390798961378No Hit
GATGTTGAGAGACGACAGCACCAAGCCCGGAGCCTGGCCCCAGAAGCAGC182420.13520475530585277No Hit
GACAAGGTCAGGCTTGCATGGTTGATGAGAAACTTGTCCTTCTCGACGCC181660.13464146392315104No Hit
ACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTC180880.13406334908300982No Hit
GGATGTTGAGAGACGACAGCACCAAGCCCGGAGCCTGGCCCCAGAAGCAG179560.13308500089200154No Hit
CATGAGACTCTGTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCCT178310.13215853480203163No Hit
CTGTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCA177810.13178794836604366No Hit
GAGTGGACAAGGTCAGGCTTGCATGGTTGATGAGAAACTTGTCCTTCTCG176940.13114312796742456No Hit
GAGACGACAGCACCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGAAGCAG176060.13049089584008572No Hit
GATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATC174360.12923090195772663No Hit
AGAAACTTGTCCTTCTCGACGCCTTGCTCGTATGTGGCCTTGGAGCCCTC173460.12856384637294827No Hit
GAGGTTCCACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGACAGC173200.12837114142623451No Hit
AACTTGTCCTTCTCGACGCCTTGCTCGTATGTGGCCTTGGAGCCCTCATT173170.12834890624007525No Hit
CACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGATG171890.127400204963946No Hit
CCTTCTCGACGCCTTGCTCGTATGTGGCCTTGGAGCCCTCATTGGAAGTT171360.12700738334179876No Hit
ACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAG170760.1265626796186132No Hit
CTTCAGGATGGGCACTGGTCACTGTCAGAGTGGACAAGGTCAGGCTTGCA170380.12628103392726234No Hit
AGCATGAGACTCTGTTTCGGGAACTGACGATACCAAAACATAGTTGTGGC169220.12542127339577022No Hit
CGACAGCACCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGAAGCAGCAGC168900.12518409807673794No Hit
TACAGATAACCCAGCTCGGATGTTGAGAGACGACAGCACCAAGCCCGGAG167580.12420574988572967No Hit
AGGTCAGGCTTGCATGGTTGATGAGAAACTTGTCCTTCTCGACGCCTTGC164600.12199705472724134No Hit
AAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAG161510.11970683055283567No Hit
AGAGACGACAGCACCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGAAGCA160570.11901012805317827No Hit
TGAGAAACTTGTCCTTCTCGACGCCTTGCTCGTATGTGGCCTTGGAGCCC160090.11865436507462981No Hit
GACGCCTTGCTCGTATGTGGCCTTGGAGCCCTCATTGGAAGTTGCCATCA158540.1175055471230671No Hit
AGCCCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGA158540.1175055471230671No Hit
GCTGTCTTCAGGATGGGCACTGGTCACTGTCAGAGTGGACAAGGTCAGGC155480.11523755813482067No Hit
CATGGTTGATGAGAAACTTGTCCTTCTCGACGCCTTGCTCGTATGTGGCC154060.11418509265661483No Hit
TGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCAC154000.11414062228429628No Hit
CCCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGACG153670.11389603523654421No Hit
CTTGCTCGTATGTGGCCTTGGAGCCCTCATTGGAAGTTGCCATCAGCATG152520.11304368643377187No Hit
GCATGGTTGATGAGAAACTTGTCCTTCTCGACGCCTTGCTCGTATGTGGC150710.11170216353549539No Hit
TTCCACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGACAGCACCA150450.11150945858878164No Hit
GCTCGTATGTGGCCTTGGAGCCCTCATTGGAAGTTGCCATCAGCATGAGA149860.11107216659431583No Hit
GACAGCACCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGAAGCAGCAGCA149720.11096840239223922No Hit
GCAGAAGCAGCAGCATCTTCCGTGATGGCCTCACACCACCTTCTCTGGGG149610.11088687337632186No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCA149460.11077569744552547No Hit
AGATAACCCAGCTCGGATGTTGAGAGACGACAGCACCAAGCCCGGAGCCT148490.11005675975970879No Hit
GCTCGGATGTTGAGAGACGACAGCACCAAGCCCGGAGCCTGGCCCCAGAA145510.10784806460122046No Hit
GTCCTTCTCGACGCCTTGCTCGTATGTGGCCTTGGAGCCCTCATTGGAAG144910.10740336087803488No Hit
CCCGGAGCCTGGCCCCAGAAGCAGCAGAAGCAGCAGCATCTTCCGTGATG144520.10711430345796426No Hit
CCACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGACAGCACCAAG144430.10704759789948644No Hit
CTTGGAGCCCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCGGGA143720.1065213651603835No Hit
GTTTTGTCCCCTAGCACTGCAGATGTAGAAGCTGCTGTCTTCAGGATGGG143600.1064324244157464No Hit
ACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCG143220.10615077872439553No Hit
CTTGTCCTTCTCGACGCCTTGCTCGTATGTGGCCTTGGAGCCCTCATTGG142320.10548372313961718No Hit
GGTTGATGAGAAACTTGTCCTTCTCGACGCCTTGCTCGTATGTGGCCTTG141940.10520207744826632No Hit
GGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGC141430.10482407928355858No Hit
GAGCCCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCGGGAACTG140990.10449796321988916No Hit
AGAGTGGACAAGGTCAGGCTTGCATGGTTGATGAGAAACTTGTCCTTCTC140470.10411255332646166No Hit
TGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACA140340.10401620085310478No Hit
GCAGATGTAGAAGCTGCTGTCTTCAGGATGGGCACTGGTCACTGTCAGAG139740.10357149712991923No Hit
ATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGT138380.10256350202403194No Hit
GATGAGAAACTTGTCCTTCTCGACGCCTTGCTCGTATGTGGCCTTGGAGC137160.10165927112022127No Hit
ACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGACAGCACCAAGCC136650.10128127295551353No Hit
ATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCT135730.10059939391329567No Hit
CACCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGAAGCAGCAGCATCTTC135730.10059939391329567No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG1055000.0137.46831
AGTCTCT569750.0137.326951
GTCTCTC680150.0114.748212
TCTCTCA702200.0111.248213
CTCTCAG752600.0103.420934
TCTCAGC752650.0103.259945
CAGCTGG753700.0103.169158
AGCTGGT768250.0101.668219
TCAGCTG785750.099.833487
CTCAGCT783650.099.1651466
CTGATGG1989450.072.636422
GATGGCT2057700.069.59554
CTGAGCC611000.068.57161145
GGCTCAA2094100.068.396677
GCTCAAA2118650.067.740338
CTCAAAC2127400.067.5878149
CGCAAAC16200.067.579199
TGGCTCA2121850.067.415746
ATGGCTC2114850.067.289725
GTGCAGG168850.064.480225145
Produced by FastQC (version 0.11.5)