FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7513603
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT4390515.843414936881812No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC2837353.7762841608746163No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2710393.607310633793135No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC910581.211908587664267No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTA906941.2070640410466191No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC903941.2030712828452608No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACA542090.7214780977914323No Hit
GTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCT227680.30302372909508263No Hit
GGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAGCCACAG199150.2650525986001656No Hit
GCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCACATT193510.2575462131816121No Hit
GTTGAAGGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAG192830.2566411879893042No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT190400.253407053846204No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC189900.25274159414597763No Hit
GCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAGCCACAGGAATATA184570.24564779374156445No Hit
GGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGA171700.22851886105773755No Hit
AGTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAG170100.22638939001701314No Hit
CAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCACA161430.21485031881508776No Hit
CTCAGGGTTGAAGGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAG159220.2119089869400872No Hit
GAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGT159140.211802513388051No Hit
GTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCA158600.2110838169118065No Hit
GAAGGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAGCCA157590.2097395883173492No Hit
CCAAGTTCTTGCTTATACCAAGGGAAGTAGTTTGAGGCACTGTCTGAATA153040.2036839050452892No Hit
CACCAAGTCCAGCTGCAGCCACAGGAATATAAATACAGCTCGAATGGATG148820.19806742517537856No Hit
ACCAAGTCCAGCTGCAGCCACAGGAATATAAATACAGCTCGAATGGATGT148220.19726887353510694No Hit
GCCACAGGAATATAAATACAGCTCGAATGGATGTCATCCTTGTTCTTCCC145110.19312971419969885No Hit
GGTCCTTTTCCAAGTTCTTGCTTATACCAAGGGAAGTAGTTTGAGGCACT143510.19100024315897446No Hit
CTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCT143120.1904811845927979No Hit
CCACATTCTCTCCATTCACCAAGTCCAGCTGCAGCCACAGGAATATAAAT140200.18659489994347586No Hit
GGTTGAAGGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCA132350.17614718264992174No Hit
AGTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCC131590.17513568390557765No Hit
CCAAGGGAAGTAGTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAG129430.1722608980005997No Hit
GCCCACATTTGAACGAATGTCTATAATAAGCTGAGGTCCTTTTCCAAGTT127990.17034437406394776No Hit
CCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCACATTCTCTCCATT125030.1664048526386076No Hit
GTAGTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTC124560.16577932052039482No Hit
GGAAGTAGTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAGCGCTG122290.16275813348136706No Hit
AGCTGAGGTCCTTTTCCAAGTTCTTGCTTATACCAAGGGAAGTAGTTTGA118030.15708841683543834No Hit
GGGTTGAAGGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAGCTGC117030.1557574974349856No Hit
GGAATATAAATACAGCTCGAATGGATGTCATCCTTGTTCTTCCCAATTAA116570.15514527451077734No Hit
GTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCACATTCTCTC114860.1528694023360031No Hit
CTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCACATTCTCTCCA114760.15273631039595784No Hit
CTCCATTCACCAAGTCCAGCTGCAGCCACAGGAATATAAATACAGCTCGA112760.15007447159505233No Hit
CTGCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAGCCACAGGAATA110630.14723961327208798No Hit
TGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGC109960.14634789727378464No Hit
GAAGTAGTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGT109490.14572236515557183No Hit
GTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGG108170.14396555154697419No Hit
CAAGTTCTTGCTTATACCAAGGGAAGTAGTTTGAGGCACTGTCTGAATAA106820.14216881035636297No Hit
CTTGCTTATACCAAGGGAAGTAGTTTGAGGCACTGTCTGAATAAGTACAC106740.14206233680432678No Hit
TGAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGG106410.14162313340217736No Hit
GCTCGAATGGATGTCATCCTTGTTCTTCCCAATTAAGATCAGTCATTGAC106100.141210548388037No Hit
GCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCACATTCT105510.14042530594176988No Hit
AGGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGG104710.13936057042140768No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG103250.13741742809674667No Hit
ACAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCAC103000.13708469824663347No Hit
GCAATTCGTTGGTCTTTCTTTTCGCCCACATTTGAACGAATGTCTATAAT101610.13523472028000416No Hit
GAACGAATGTCTATAATAAGCTGAGGTCCTTTTCCAAGTTCTTGCTTATA101600.1352214110859996No Hit
AAGGGAAGTAGTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAGCG101530.13512824672796792No Hit
GCTTATACCAAGGGAAGTAGTTTGAGGCACTGTCTGAATAAGTACACTTG101310.1348354444598683No Hit
CTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCACATTCTCTCCATTCA100830.134196603147651No Hit
CCATTCACCAAGTCCAGCTGCAGCCACAGGAATATAAATACAGCTCGAAT100070.13318510440330691No Hit
GAGGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTG99720.13271928261314844No Hit
CCAGCTGCAGCCACAGGAATATAAATACAGCTCGAATGGATGTCATCCTT97710.13004413461823844No Hit
CTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCACATTCTC97670.12999089784222032No Hit
GCTGCAGCCACAGGAATATAAATACAGCTCGAATGGATGTCATCCTTGTT97150.12929881975398488No Hit
GTCCTTTTCCAAGTTCTTGCTTATACCAAGGGAAGTAGTTTGAGGCACTG96230.12807437390556833No Hit
GTTGGTCTTTCTTTTCGCCCACATTTGAACGAATGTCTATAATAAGCTGA96040.12782149921948233No Hit
GTTCAATGTAACAGCAATTCGTTGGTCTTTCTTTTCGCCCACATTTGAAC95350.1269031648331699No Hit
CTGGACACTCAGGGTTGAAGGATGCTGCTCCACATTCTCTCCATTCACCA95340.12688985563916538No Hit
ATAAGCTGAGGTCCTTTTCCAAGTTCTTGCTTATACCAAGGGAAGTAGTT94700.12603806722287564No Hit
GCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGAC93660.12465391104640478No Hit
CAGCAATTCGTTGGTCTTTCTTTTCGCCCACATTTGAACGAATGTCTATA92970.12373557666009237No Hit
CTGAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGG92430.12301688018384789No Hit
CATTCACCAAGTCCAGCTGCAGCCACAGGAATATAAATACAGCTCGAATG91740.12209854579753548No Hit
CCTTTTCCAAGTTCTTGCTTATACCAAGGGAAGTAGTTTGAGGCACTGTC90590.12056798848701482No Hit
CAGGGTTGAAGGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAGCT90020.11980936442875674No Hit
GTCTTTCTTTTCGCCCACATTTGAACGAATGTCTATAATAAGCTGAGGTC89900.11964965410070241No Hit
GATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAGCCACAGG89310.11886441165443529No Hit
GTTCTTGCTTATACCAAGGGAAGTAGTTTGAGGCACTGTCTGAATAAGTA89230.11875793810239908No Hit
ACCAAGGGAAGTAGTTTGAGGCACTGTCTGAATAAGTACACTTGATAACA89170.11867808293837191No Hit
GGTCTTTCTTTTCGCCCACATTTGAACGAATGTCTATAATAAGCTGAGGT88910.1183320438942542No Hit
GTCTATAATAAGCTGAGGTCCTTTTCCAAGTTCTTGCTTATACCAAGGGA88080.11722738079187842No Hit
CCTGGACACTCAGGGTTGAAGGATGCTGCTCCACATTCTCTCCATTCACC87620.11661515786767014No Hit
GGGAAGTAGTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAGCGCT87430.11636228318158412No Hit
GGACACTCAGGGTTGAAGGATGCTGCTCCACATTCTCTCCATTCACCAAG84860.11294182032242056No Hit
CTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAGCCACAGGAATATAA84500.11246268933825755No Hit
CTTATACCAAGGGAAGTAGTTTGAGGCACTGTCTGAATAAGTACACTTGA82470.10976092295533849No Hit
AGTAGTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCT82260.10948142988124339No Hit
CACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATG82210.10941488391122076No Hit
GGTTGGGTCTCTGTGATGTGCAGGGAGAAATGTTTGGCTGTCTTGTTCAA80860.10761814272060954No Hit
GGGAGAAATGTTTGGCTGTCTTGTTCAATGTAACAGCAATTCGTTGGTCT80670.10736526803452352No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG80370.10696599221438768No Hit
TCTCCATTCACCAAGTCCAGCTGCAGCCACAGGAATATAAATACAGCTCG79440.10572823717196662No Hit
CTTTCTTTTCGCCCACATTTGAACGAATGTCTATAATAAGCTGAGGTCCT79270.10550198087388968No Hit
TATACCAAGGGAAGTAGTTTGAGGCACTGTCTGAATAAGTACACTTGATA78980.10511601424775838No Hit
CACTCAGGGTTGAAGGATGCTGCTCCACATTCTCTCCATTCACCAAGTCC78880.1049829223077131No Hit
ACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGAT77150.10268043174492983No Hit
CTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACATT76810.10222791914877588No Hit
GCCGAGTCTTCAGGTTGGGTCTCTGTGATGTGCAGGGAGAAATGTTTGGC76800.10221460995477136No Hit
CCCACATTTGAACGAATGTCTATAATAAGCTGAGGTCCTTTTCCAAGTTC76230.10145598589651329No Hit
AACAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCA75760.1008304537783005No Hit
TACCAAGGGAAGTAGTTTGAGGCACTGTCTGAATAAGTACACTTGATAAC75450.10041786876416016No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)