FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23185241
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC9432274.068221676022259No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC8833103.80979434287528No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC5840302.5189731691812045No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCA1161520.5009738738536296No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC899310.38788037614101145No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA759470.3275661443415663No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT667220.28777790146757587No Hit
GTCTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATT658860.28417215934913076No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAG653910.28203718046320936No Hit
GGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTG643430.27751706354917766No Hit
CCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACTT639570.2758522113270248No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACT638290.27530013597874614No Hit
CCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATAT622520.26849839516440654No Hit
GTCCAGGATCCTGCTCCACCTCCTTCTGTTGGCTCCAAACCCAGCTTAAC609600.26292588461771865No Hit
GCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGG590890.25485609573780144No Hit
GAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACA551870.23802642379261874No Hit
GATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTT549090.23682738514557602No Hit
CCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCC542510.23398937280833093No Hit
CCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATATC538610.23230726823154435No Hit
GAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGGTT538500.2322598242563017No Hit
CCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGAC536690.23147915520912635No Hit
GTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGA534870.23069417307329262No Hit
GGAATATCTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAGATCAG528720.2280416235483599No Hit
ATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTC525530.2266657482663217No Hit
GCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATA520100.2243237411247957No Hit
AGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGGT517220.22308157159116873No Hit
GCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTT505470.21801369241751684No Hit
CTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCC487060.21007329619735243No Hit
GTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGA477800.20607937609964888No Hit
AATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATA461390.19900159761116998No Hit
ATGAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAG455790.19658626796245077No Hit
CCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCA446970.19278212376571804No Hit
GGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAATA423280.1825644167339041No Hit
ATTTTAACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTC422840.1823746408329333No Hit
CTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATC417510.18007576457799165No Hit
ATGGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTT412310.17783295847560954No Hit
GTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAA406900.17549957751140047No Hit
GTTGGCTCCAAACCCAGCTTAACTGAAGCCACAGGATCACCAGTAAAACT398150.1717256249352767No Hit
GACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGGCACTCG396960.17121236738492387No Hit
GATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAA396780.1711347317890722No Hit
CTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACC393590.16975885650703393No Hit
GGCGGACTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACAC393360.1696596554678901No Hit
GCCTTGGAGCCCTCATTGGAAGTTGCCATCAGCATGAGACTCTGTTTCGG388680.1676411299757462No Hit
CAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGG382760.16508778148995734No Hit
CTTTTGACTAGATATCTCGAGCTCTGGGTTACTTTCACATCTACGAGGCC380330.16403970094595954No Hit
CTTTCATTTTAACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCT379930.16386717739962245No Hit
GAAAAGTACTGTTGCTGTAAGTGCAGTTGAGAGAAACAATGGCTCCCTCT379240.163569574282191No Hit
CATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCT377720.16291398480611008No Hit
CTCCAAACCCAGCTTAACTGAAGCCACAGGATCACCAGTAAAACTCTCAA377570.16284928847623364No Hit
AGAGACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTAACAT373080.16091271166859986No Hit
CCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTA369840.15951527094326948No Hit
GCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAATATCTCCTTTT365710.15773396532733905No Hit
AGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATG364870.15737166588003118No Hit
ACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGGTTACTTT363550.1568023381771188No Hit
CATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTC352070.15185091239724444No Hit
CTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGGTTACTTTCACATCT350010.15096241613360845No Hit
TGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAG346820.1495865408515702No Hit
GTTGAGAGAAACAATGGCTCCCTCTGGAACACTGAGTGGTCCAGGATCCT344970.14878861944976118No Hit
CCCGTCCTTTTGACTAGATATCTCGAGCTCTGGGTTACTTTCACATCTAC342320.14764565095527796No Hit
GAGAAACAATGGCTCCCTCTGGAACACTGAGTGGTCCAGGATCCTGCTCC338840.14614469610214534No Hit
CGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAA338480.14598942491044195No Hit
AGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACTTTC338040.14579964900947115No Hit
GGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCT335430.14467393286962169No Hit
GGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACG334010.14406147428012503No Hit
GGCTCCAAACCCAGCTTAACTGAAGCCACAGGATCACCAGTAAAACTCTC330960.14274598223930474No Hit
GCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGAT330290.14245700529919011No Hit
CCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATAT329990.1423276126394373No Hit
CCCAGCTCGGATGTTGAGAGACGACAGCACCAAGCCCGGAGCCTGGCCCC329550.1421378367384665No Hit
CTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGGTTACTTTC326690.14090429338215635No Hit
GGTCCAGGATCCTGCTCCACCTCCTTCTGTTGGCTCCAAACCCAGCTTAA326400.14077921381106195No Hit
AAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGGTTAC322580.1391316139435428No Hit
GGAAGTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGACGATACCAAA322210.138972029663181No Hit
AGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAATATCTCCT321760.13877794067355176No Hit
GAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAA320920.1384156412262439No Hit
CTCTAGAGACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTA320150.138083533399545No Hit
GAAACAATGGCTCCCTCTGGAACACTGAGTGGTCCAGGATCCTGCTCCAC320000.13801883706966858No Hit
GGTTGGTGCTGGCGGACTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCT317710.13703113976688877No Hit
CCTCAGGAATATCTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAG316610.1365567000144618No Hit
GCTGTAAGTGCAGTTGAGAGAAACAATGGCTCCCTCTGGAACACTGAGTG313890.1353835398993696No Hit
GACTAGATATCTCGAGCTCTGGGTTACTTTCACATCTACGAGGCCTACAG311900.1345252352563426No Hit
TTTCATTTTAACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCTG311850.13450366981305045No Hit
GGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACA311760.13446485201512462No Hit
ACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCG307250.132519649030174No Hit
GTGCTGGCGGACTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGA307100.13245495270029758No Hit
CTCTGTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTC306860.13235143857249532No Hit
CCAGGATCCTGCTCCACCTCCTTCTGTTGGCTCCAAACCCAGCTTAACTG305680.1318424941108009No Hit
GCCACAGGATCACCAGTAAAACTCTCAAGGATTTCATCATTCTTTTCTGC305210.13163977894385484No Hit
GACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTAACATCAT305070.13157939570263685No Hit
CTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAGATCAGCCGTAGC303390.1308547968080211No Hit
GCCATCAGCATGAGACTCTGTTTCGGGAACTGACGATACCAAAACATAGT302730.13057013295656492No Hit
GGAACACTGAGTGGTCCAGGATCCTGCTCCACCTCCTTCTGTTGGCTCCA301290.1299490481897514No Hit
GAGACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTAACATC298810.12887940220246147No Hit
GCCAGGAAACAAAAGGCCACACGACAGAGGAGCCTGATTCCCATGGCTGC298520.12875432263136707No Hit
TATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCC296290.12779250386053784No Hit
ATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTC294540.12703771334531308No Hit
GTTGTGGCCTGAAAGTCCAGGGAACGGCACTCGATCTTCACAGAGGTTCC294320.12694282539482768No Hit
GTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGG294020.12681343273507487No Hit
CTCGAGCTCTGGGTTACTTTCACATCTACGAGGCCTACAGCCAGGAAACA292300.1260715814858254No Hit
CCAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGG291310.12564458570864112No Hit
GGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTC290570.1253254171479175No Hit
CTGCTCCACCTCCTTCTGTTGGCTCCAAACCCAGCTTAACTGAAGCCACA288680.12451024339147478No Hit
GGTTCCACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGACAGCAC288620.12448436485952422No Hit
GCATGAGACTCTGTTTCGGGAACTGACGATACCAAAACATAGTTGTGGCC285880.12330257856711518No Hit
ATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGAT284440.12268149380030167No Hit
CGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGG281600.12145657662130836No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGT276710.1193474762673375No Hit
GGCTTGCATGGTTGATGAGAAACTTGTCCTTCTCGACGCCTTGCTCGTAT275960.11902399461795544No Hit
GTTACTTTCACATCTACGAGGCCTACAGCCAGGAAACAAAAGGCCACACG274180.11825626483675541No Hit
GCTCCAAACCCAGCTTAACTGAAGCCACAGGATCACCAGTAAAACTCTCA271050.11690626808666772No Hit
AGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAAT270950.11686313720008346No Hit
TGAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGA270580.11670355291972165No Hit
CATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGA270270.11656984717131041No Hit
GCTCTGGGTTACTTTCACATCTACGAGGCCTACAGCCAGGAAACAAAAGG270190.11653534246204297No Hit
TGGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTT269070.11605227653229913No Hit
CTTTCACATCTACGAGGCCTACAGCCAGGAAACAAAAGGCCACACGACAG268360.11574604723755082No Hit
GTTGCCATCAGCATGAGACTCTGTTTCGGGAACTGACGATACCAAAACAT267660.11544413103146092No Hit
ATTGAAAAGTACTGTTGCTGTAAGTGCAGTTGAGAGAAACAATGGCTCCC266340.11487480332854852No Hit
CAGGGAACGGCACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCC265630.1145685740338002No Hit
ATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCG259790.11204973025727875No Hit
GACGACAGCACCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGAAGCAGCA257680.11113966855035064No Hit
CACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTAACATCATAT257290.11097145809267196No Hit
GGGTTACTTTCACATCTACGAGGCCTACAGCCAGGAAACAAAAGGCCACA256730.11072992512780006No Hit
GTGCAGTTGAGAGAAACAATGGCTCCCTCTGGAACACTGAGTGGTCCAGG256560.1106566026206068No Hit
GTTCCACTCTTACAGATAACCCAGCTCGGATGTTGAGAGACGACAGCACC254570.1097982979775798No Hit
AGAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAAC253670.10941011999832134No Hit
GGATCCTGCTCCACCTCCTTCTGTTGGCTCCAAACCCAGCTTAACTGAAG252930.10909095143759773No Hit
AGTAAAACTCTCAAGGATTTCATCATTCTTTTCTGCCCTGGTTCACTGAG252890.10907369908296402No Hit
GATAACCCAGCTCGGATGTTGAGAGACGACAGCACCAAGCCCGGAGCCTG250820.10818088973066961No Hit
ACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCT250440.10801699236164938No Hit
CTCAGGAATATCTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAGA249620.10766331909165835No Hit
GACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCG248580.10721475787118193No Hit
ATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCT245220.10576556008195041No Hit
CCCAGCTTAACTGAAGCCACAGGATCACCAGTAAAACTCTCAAGGATTTC245080.10570517684073244No Hit
GCACCAAGCCCGGAGCCTGGCCCCAGAAGCAGCAGAAGCAGCAGCATCTT244970.10565773286548973No Hit
TGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACAC244690.10553696638305377No Hit
CTCGGATGTTGAGAGACGACAGCACCAAGCCCGGAGCCTGGCCCCAGAAG243280.10492882088221556No Hit
CACAGGATCACCAGTAAAACTCTCAAGGATTTCATCATTCTTTTCTGCCC242820.1047304188039279No Hit
GTTGCTGTAAGTGCAGTTGAGAGAAACAATGGCTCCCTCTGGAACACTGA242590.10463121776478407No Hit
GACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGG242200.10446300730710542No Hit
ACTCGATCTTCACAGAGGTTCCACTCTTACAGATAACCCAGCTCGGATGT241580.10419559581028293No Hit
ACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACT240380.10367802517127168No Hit
CTTTTTCTTTCATTTTAACATCATATGAGAAATAGATCAGCCGTAGCCCC239430.1032682817487211No Hit
GGATCACCAGTAAAACTCTCAAGGATTTCATCATTCTTTTCTGCCCTGGT238560.10289304303543792No Hit
CATGGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTT237940.10262563153861544No Hit
ACTGAAGCCACAGGATCACCAGTAAAACTCTCAAGGATTTCATCATTCTT237440.10240997710569408No Hit
TGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCT236780.10212531325423789No Hit
CTGAGTGGTCCAGGATCCTGCTCCACCTCCTTCTGTTGGCTCCAAACCCA235290.10148266304413225No Hit
GGAACTGACGATACCAAAACATAGTTGTGGCCTGAAAGTCCAGGGAACGG233980.1009176484298783No Hit
GGCCTTTTCTGGAATACTGTCTGTACCACATGAAGTATTGAAAAGTACTG232090.10010247467343557No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)