FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15695572
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC6834154.354189831374097No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG6140413.9121925597869254No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC5527373.521611063298617No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGGGTG2140191.363562920803396No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGA1084120.6907171016131174No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGATG584850.372621016933948No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT396230.2524469958788377No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGGGTGA386960.24654087152733267No Hit
GTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACAG367570.23418706881150939No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC355170.22628675144811544No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC345580.22017674793884542No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC304440.19396553371868192No Hit
GGTTGGGGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTG290700.18521147238214702No Hit
CAAAAACTTGAGCTCTTCTTCCAGCTTCTTACGATACCAGTAAACATAAC238860.15218304882421615No Hit
GAAAAATTGCACCGCAGCGTGGAATTGGCTCCCTCCTGGAGAATCAGGTC238780.1521320790347749No Hit
CCAAAAACTTGAGCTCTTCTTCCAGCTTCTTACGATACCAGTAAACATAA237490.15131019118003472No Hit
GTTTTTGGAGCATTGGGCTAAAAATCGCTCATTGATTATTTCTGCTTTCT232250.14797166997163277No Hit
GGAGCATTGGGCTAAAAATCGCTCATTGATTATTTCTGCTTTCTGAATAA229420.1461686136701485No Hit
GCTAAAAATCGCTCATTGATTATTTCTGCTTTCTGAATAAGTTCTTCATT226860.14453758040802847No Hit
GGCTGCACCAGGGTCGCTGGCCATTGATGAATAGTCTGAGATCTGACCCC226730.1444547545001864No Hit
GGCCAATACCAACGGAGTAGAAGAGCAGCTGGAGGCCCCTGCCTGCAGCC216880.13817909917523236No Hit
GCCTGTCGGTACCAGTATAGGTTGGGGTTTGATGTTCCCTCCACAGTGCA216750.13809627326739032No Hit
GGTTGATGAGCTGTCCCCAAGGGTTTTGATGAAACCACTGCAAATTGTTC216210.13775222718866187No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGGGTGACC215520.13731261275473108No Hit
GCTGGCCATTGATGAATAGTCTGAGATCTGACCCCAAAGAAAGTGCCCAG215090.13703865013648434No Hit
GCCCACAGGCTGCACCAGGGTCGCTGGCCATTGATGAATAGTCTGAGATC213570.13607022413710057No Hit
GTTGGGGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGC212630.13547132911116586No Hit
GTTCTTCATTCTGAAAGTAAACCAAAAACTTGAGCTCTTCTTCCAGCTTC211490.13474500961162802No Hit
GGATGAGTTTTTGGAGCATTGGGCTAAAAATCGCTCATTGATTATTTCTG208940.13312034757318816No Hit
GTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACAGGCTGCAC208130.13260427845509548No Hit
GGTACCAGTATAGGTTGGGGTTTGATGTTCCCTCCACAGTGCACTCCAGA202840.12923390112829275No Hit
GGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACA201490.1283737859314716No Hit
GATTATTTCTGCTTTCTGAATAAGTTCTTCATTCTGAAAGTAAACCAAAA201340.12827821757626928No Hit
GCAAATTGTTCACAGAGTCAGAAAAATTGCACCGCAGCGTGGAATTGGCT199110.1268574346955944No Hit
GGCCCCTGCCTGCAGCCTGTCGGTACCAGTATAGGTTGGGGTTTGATGTT196830.12540479569651874No Hit
GGATCTCCAAGGTACAGGATGAGTTTTTGGAGCATTGGGCTAAAAATCGC195750.12471670353906184No Hit
GTCTGAGATCTGACCCCAAAGAAAGTGCCCAGGAGAAGGGCAAGGAGAGA193530.12330229188206714No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG191930.12228289609324208No Hit
GAATAAGTTCTTCATTCTGAAAGTAAACCAAAAACTTGAGCTCTTCTTCC190170.12116156072553456No Hit
CTGAGATCTGACCCCAAAGAAAGTGCCCAGGAGAAGGGCAAGGAGAGAGC189790.12091945422568862No Hit
AGTTTTTGGAGCATTGGGCTAAAAATCGCTCATTGATTATTTCTGCTTTC183190.11671444659678538No Hit
GCTGGAGGCCCCTGCCTGCAGCCTGTCGGTACCAGTATAGGTTGGGGTTT182470.11625571849181411No Hit
GATGAAACCACTGCAAATTGTTCACAGAGTCAGAAAAATTGCACCGCAGC181000.11531914861083115No Hit
GATGAATAGTCTGAGATCTGACCCCAAAGAAAGTGCCCAGGAGAAGGGCA178040.11343326640150483No Hit
GGAACACAATCCATCTTTGCTTTCTGTTCACTTGCTTTGACCAGAAGTCT176870.11268783323092654No Hit
CAGAAAAATTGCACCGCAGCGTGGAATTGGCTCCCTCCTGGAGAATCAGG174020.11087203448208195No Hit
GATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACAGGCTG173580.11059170064015506No Hit
GCTCTTCTTCCAGCTTCTTACGATACCAGTAAACATAACTATGTGCTTTT171640.10935568324620473No Hit
GCAGCTGGAGGCCCCTGCCTGCAGCCTGTCGGTACCAGTATAGGTTGGGG171250.10910720552267862No Hit
CCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACAGGCTGCACCAGG170110.10838088602314079No Hit
CTCTGCTCCACTTGTATTCCTCTCACACAGCAAACCTGGGCACTCAAGAG169100.107737392431445No Hit
AGAAAAATTGCACCGCAGCGTGGAATTGGCTCCCTCCTGGAGAATCAGGT168570.10739971757639671No Hit
GGGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCAC167240.1065523448269359No Hit
CTCCAGAGAGAGCGGGCTGCCCACAGGCTGCACCAGGGTCGCTGGCCATT163560.10420773451263834No Hit
GGCTGCCCACAGGCTGCACCAGGGTCGCTGGCCATTGATGAATAGTCTGA162870.10376812007870755No Hit
GCTCATTGATTATTTCTGCTTTCTGAATAAGTTCTTCATTCTGAAAGTAA162000.10321382361853394No Hit
GTTTTGATGAAACCACTGCAAATTGTTCACAGAGTCAGAAAAATTGCACC161730.10304180057916972No Hit
GCTCCCTCCTGGAGAATCAGGTCTGGAGGACTCTGCTCCACTTGTATTCC161380.10281880775036424No Hit
AGTTCTTCATTCTGAAAGTAAACCAAAAACTTGAGCTCTTCTTCCAGCTT159300.10149359322489172No Hit
GCTGTCCCCAAGGGTTTTGATGAAACCACTGCAAATTGTTCACAGAGTCA157840.10056339456758887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCT1278550.0139.313951
TCTGATG923450.0138.014051
GTCTCTC1479250.0119.8527762
TCTCTCA1513550.0116.8584753
CTCTCAG1631700.0108.2543264
TCTCAGC1647300.0106.9210745
AGCTGGT1673900.0106.152249
CTCAGCT1662100.0105.5975656
TCAGCTG1693200.0104.687637
CTGATGG1667800.076.0518342
TGATGGC1716700.073.593873
GATGGCT1719600.072.794954
GGCTCAA1736900.072.3212667
CTCAAAC1759850.071.663769
GCTCAAA1757500.071.6399468
ATGGCTC1758550.070.457025
AGGAAAT1301350.066.18724145
TGCACAG493100.063.610786145
CAGCTGG2825650.063.548588
TCAAACA1779650.059.5571449
Produced by FastQC (version 0.11.5)