FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10477038
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTC8467068.081539839790597No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTT3467183.3093131856541897No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTCA1790421.7088990227963285No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC1784761.7034967325688808No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC1654071.5787572785361665No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGTTGTTG1574771.5030679472576125No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTCACT1178341.1246881036415064No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTTA738450.7048270703990956No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGAAAAG491350.46897796877323533No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTTACT467180.44590847145920437No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGTTGTTGC340640.3251300606144599No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC302750.28896525907417725No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC286190.2731592650518209No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGTTGTTGCCA221950.21184422543852566No Hit
GTCAGGAAGGCCTCTTCTGAGAGAGTCACTGGCCCTTCCATCTGGGTCAC200660.19152359665012192No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCCTTCCAG167180.1595680000397059No Hit
CTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTCACTGA167060.15945346385113807No Hit
GTTTATAGTCAGGAAGGCCTCTTCTGAGAGAGTCACTGGCCCTTCCATCT151470.1445733040197048No Hit
GTCACATTCTGTCCTTCCTTTCTGAACAGGTACTTTGGGGACTGAGTGAT146450.13978187346461854No Hit
TCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTCAC142870.13636487717234586No Hit
GGAAGAGAATGAGAAAAGAGACTTGGCTCGGGGGCTTCTCCTGGCCTAAT140960.13454184283764173No Hit
GGAGGAGCTGTAGACCTTCTCCAGGATATTGGACATACCAGAAAAGGGAA140940.1345227534728804No Hit
GGTACTTTGGGGACTGAGTGATTCCACCATCCACGGTGTTTGCTCCCAGG137560.13129665082822073No Hit
GAGTGATTCCACCATCCACGGTGTTTGCTCCCAGGAAACAAAGGACCACA134730.1285955057144968No Hit
GTGCAAAGGGGGCCTTCTTGGAGCTCCAAGCACTTGTTGGAAGAGAATGA134340.12822326310165144No Hit
CAAAGGACCACACAGCAGAGCACCTGGTTGCTCATAGTGCAAAGGGGGCC130590.1246440072089077No Hit
CCAGGATATTGGACATACCAGAAAAGGGAAGGGTATCCTGTGGCTGTGTA124230.11857358921481434No Hit
GTTTGCTCCCAGGAAACAAAGGACCACACAGCAGAGCACCTGGTTGCTCA124160.11850677643814979No Hit
GGGAAGGGTATCCTGTGGCTGTGTACGTGCAGTTTATAGTCAGGAAGGCC122160.1165978399620198No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT121030.11551929085300637No Hit
GGACATACCAGAAAAGGGAAGGGTATCCTGTGGCTGTGTACGTGCAGTTT118680.11327629049355363No Hit
GCCTCTTCTGAGAGAGTCACTGGCCCTTCCATCTGGGTCACTGAATCTCC116540.11123372846409453No Hit
GCTCCAAGCACTTGTTGGAAGAGAATGAGAAAAGAGACTTGGCTCGGGGG115710.11044151982650058No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC114220.10901936215178373No Hit
GCAAAGGGGGCCTTCTTGGAGCTCCAAGCACTTGTTGGAAGAGAATGAGA114190.10899072810464179No Hit
CCCAGGAAACAAAGGACCACACAGCAGAGCACCTGGTTGCTCATAGTGCA112630.1075017576532604No Hit
GTTCACAACTCAGGGTCACATTCTGTCCTTCCTTTCTGAACAGGTACTTT110590.1055546424476078No Hit
CCAAGCACTTGTTGGAAGAGAATGAGAAAAGAGACTTGGCTCGGGGGCTT107060.10218536956723837No Hit
GGGCCTTCTTGGAGCTCCAAGCACTTGTTGGAAGAGAATGAGAAAAGAGA106840.10197538655486407No Hit
GAGAGAGTCACTGGCCCTTCCATCTGGGTCACTGAATCTCCACGGGTTCT106240.10140270561202508No Hit
GTCACTGGCCCTTCCATCTGGGTCACTGAATCTCCACGGGTTCTTCCAAG106080.10124999069393469No Hit
GCCTTCGTGGCTTTCAGGAGGAGCTGTAGACCTTCTCCAGGATATTGGAC105170.10038142459729553No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)