FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16114241
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA6770034.201271409556305No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC4367972.710627202360943No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3494402.1685166555471027No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC3317502.058737982136422No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC2612541.6212615909120387No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTAA1194130.7410401768224765No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTC1159250.7193947266892683No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTAACA663540.4117724191911986No Hit
CTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACA452040.28052205499470934No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGC444030.27555129652088484No Hit
GGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGG392070.24330652619630055No Hit
GTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGAC384980.23890669129250333No Hit
GTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTC336320.20870979899084297No Hit
GGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGG326240.20245446248445706No Hit
GTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCT320260.19874345927927972No Hit
GGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGG319400.1982097698551238No Hit
GTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCT316800.19659629020069885No Hit
GTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGC299160.1856494513145236No Hit
GTCGATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGT288690.179152092859974No Hit
GCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTTATGG281650.17478328641106958No Hit
GTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGA276080.17132671653601309No Hit
CTTGTCGATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGC272590.16916093038449653No Hit
GTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTTATGGTTCATA269570.16728681170897222No Hit
AATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATG267700.16612634749598199No Hit
GTCAGTGGTACCCTCAGAAGCTGAGTAATAAATCAGCCTCAGTCCCATGC267250.1658470914019469No Hit
GTAATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACA263520.16353236866694498No Hit
GTTTAATCTGGAGACATTGTAGCCATTGGGGACTTCTCCTTTGTCAGTGG260710.16178856950197035No Hit
GCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGA259830.1612424686958573No Hit
GTCTTGTCGATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACT258410.16036126057690211No Hit
GAAAAGGCCACACAGCACAGGAGCCCGATGCTCATGGCAGGTGCTACAGG254860.15815824027951425No Hit
CCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCAC253820.15751284841774427No Hit
GCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAG250320.15534085657524918No Hit
GCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGG247410.15353500050048896No Hit
CCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGC246860.1531936874966683No Hit
CTCAGAAGCTGAGTAATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTC246630.15305095660416151No Hit
TGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAG245960.15263517530859816No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT243230.15094102167145196No Hit
CCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCAC242920.1507486452511167No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG242080.15022736720891788No Hit
ATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGA237880.14762097699792376No Hit
ATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGA237470.1473665436678029No Hit
GCCGACTCCAGCCTGAGCGAGAACTCCCGTTTGTTTAATCTGGAGACATT236430.14672115180603293No Hit
CCAAGTTCTTGCTTATACCAAGGGAAGTAGTTTGAGGCACTGTCTGAATA235410.1460881713262201No Hit
GGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGA235080.14588338352392768No Hit
CTTCTCCTTTGTCAGTGGTACCCTCAGAAGCTGAGTAATAAATCAGCCTC230940.14331422745880493No Hit
CCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTTATGGTTCATATC226430.1405154608274755No Hit
GATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGTC224880.13955357872579913No Hit
CACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACACAGCAC224160.139106768975343No Hit
CTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCT224150.13910056328436443No Hit
CTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGAC224010.13901368361066463No Hit
CCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACACAGCACAG223880.1389330096279434No Hit
GCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCACATT223260.1385482567872728No Hit
GGAGAGAAAAGGCCACACAGCACAGGAGCCCGATGCTCATGGCAGGTGCT221910.13771048850516757No Hit
GGTCCTTTTCCAAGTTCTTGCTTATACCAAGGGAAGTAGTTTGAGGCACT213500.13249150239220078No Hit
GGTACCCTCAGAAGCTGAGTAATAAATCAGCCTCAGTCCCATGCCTGGGT211230.13108281054006826No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTCA208480.12937624552096497No Hit
GGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGGACTTGCC208340.12928936584726516No Hit
GTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTG207780.12894184715246595No Hit
CAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCTGCTCCACA207130.12853847723885972No Hit
GTCTGAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCA206240.1279861707417681No Hit
GGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAGCCACAG205890.1277689715575186No Hit
CTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACT204740.1270553170949845No Hit
CATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACAC204070.12663953579942117No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC202610.12573350491655177No Hit
GAATAAGTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGT202120.12542942605860247No Hit
CATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTC201180.12484609110661804No Hit
CTCAGGGTTGAAGGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAG200920.12468474314117556No Hit
CTTGCCCACAGGAGAGAAAAGGCCACACAGCACAGGAGCCCGATGCTCAT199930.12407037973429838No Hit
AATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGT198140.1229595610491366No Hit
GTTGAAGGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAG196920.12220246674975259No Hit
GCTGAGTAATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCA196770.12210938138507424No Hit
GAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACA196740.12209076431213856No Hit
TATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTC195820.12151984074211127No Hit
AGTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAG192840.11967054483050117No Hit
GTAGTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAGCGCTGTCTC192800.11964572206658694No Hit
CACTGGACTTGCCCACAGGAGAGAAAAGGCCACACAGCACAGGAGCCCGA192790.11963951637560838No Hit
GTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGCATTCACTGG189670.11770334079029847No Hit
GGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAG188810.11716965136614253No Hit
CACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGT187100.11610847820880922No Hit
GCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAGCCACAGGAATATA185520.11512797903419714No Hit
CCTGGGTCTTGTCGATACCAGTACATGGAGTTATGGTTCATATCCTGGGC185450.11508453919734725No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT185340.1150162765965831No Hit
ATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACC183050.11359517336249345No Hit
CTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGGATGCT182700.11337797417824394No Hit
GGGGACTTCTCCTTTGTCAGTGGTACCCTCAGAAGCTGAGTAATAAATCA182630.11333453434139405No Hit
CCAAGGGAAGTAGTTTGAGGCACTGTCTGAATAAGTACACTTGATAACAG181270.11249056036831025No Hit
GGGGTCTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAA180990.11231680102091063No Hit
CCTTTGTCAGTGGTACCCTCAGAAGCTGAGTAATAAATCAGCCTCAGTCC177970.1104426823453863No Hit
CCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTTATGGT177020.10985314170242333No Hit
CAAGTTCTTGCTTATACCAAGGGAAGTAGTTTGAGGCACTGTCTGAATAA176000.10922016122261048No Hit
CCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAAT176000.10922016122261048No Hit
AGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTC175540.10893469943759684No Hit
GCCCACATTTGAACGAATGTCTATAATAAGCTGAGGTCCTTTTCCAAGTT174930.10855615228790486No Hit
CTGAGTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCA174420.1082396620479984No Hit
GAAGGATGCTGCTCCACATTCTCTCCATTCACCAAGTCCAGCTGCAGCCA173830.10787352628026353No Hit
ATGGTTCATATCCTGGGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCA172900.10729639701925768No Hit
GCCATTGGGGACTTCTCCTTTGTCAGTGGTACCCTCAGAAGCTGAGTAAT172410.10699231816130837No Hit
ATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTACATGGAGTT171050.10614834418822457No Hit
GAGTAATAAATCAGCCTCAGTCCCATGCCTGGGTCTTGTCGATACCAGTA170750.10596217345886785No Hit
GTTCAATGTAACAGCAATTCGTTGGTCTTTCTTTTCGCCCACATTTGAAC169270.10504373119404134No Hit
CTTGCTTATACCAAGGGAAGTAGTTTGAGGCACTGTCTGAATAAGTACAC167510.10395152958181525No Hit
GTGACACCAGCATTCACTGGACTTGCCCACAGGAGAGAAAAGGCCACACA167130.10371571332463006No Hit
CGATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAGTGT167030.10365365641484448No Hit
CTCCCGTTTGTTTAATCTGGAGACATTGTAGCCATTGGGGACTTCTCCTT165500.1027041856951252No Hit
GCGAGAACTCCCGTTTGTTTAATCTGGAGACATTGTAGCCATTGGGGACT164960.1023690783822831No Hit
GGCACACTGCAGTGTCATGCTCTGTCCTGTCTTCAGGACCTGGAATTTTG163860.10168645237464179No Hit
CTCTGTCCTGTCTTCAGGACCTGGAATTTTGGGGTCTGAGTGACACCAGC163310.10134513937082112No Hit
TGTCGATACCAGTACATGGAGTTATGGTTCATATCCTGGGCACACTGCAG162740.10099141498504335No Hit
GTTGGTCTTTCTTTTCGCCCACATTTGAACGAATGTCTATAATAAGCTGA161750.10037705157816618No Hit
GTACACTTGATAACAGCGCTGTCTCCCTCCTGGACACTCAGGGTTGAAGG161260.10007297272021685No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCT1384700.0130.09451
TCTGATG1059600.0127.1836851
GTCTCTC1491200.0120.355612
TCTCTCA1512050.0118.499423
TCAGCTG1653850.0108.201437
CTCTCAG1660150.0107.721974
TCTCAGC1679150.0106.347635
CAGCTGG1688750.0105.374498
AGCTGGT1706050.0104.8371669
CTCAGCT1735750.0102.64756
CTGATGG1704700.078.8413852
TGATGGC1757600.076.274563
GGCTCAA1771500.075.100657
CTCAAAC1806200.073.9881449
GCTCAAA1806500.073.907628
GATGGCT1800550.073.890714
ATGGCTC1792000.073.6444855
TGGCTCA1804000.073.525486
CTGTGAT1417800.065.59614145
TAGCTGC218500.057.49283145
Produced by FastQC (version 0.11.5)