FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613197
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT3003458.312444630060304No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT380221.0523090769753214No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT299400.8286290506717457No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT216090.598057620439738No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA135620.37534626537108273No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT123930.3429926461247477No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC116990.3237852793523298No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC113820.3150118855960525No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA108520.3003434354672607No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91190.25238037117821144No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG75430.20876248928580424No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG58900.16301353067657257No Hit
GCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGGAGACTGTGTCAACAC48350.13381501202397766No Hit
GTTGGTACCAGGCTAAGTAGCTGCTAACACTCTGACTGGCCCTGCAGGAG47450.13132414313418284No Hit
GGTTTCTGTTGGTACCAGGCTAAGTAGCTGCTAACACTCTGACTGGCCCT44060.12194187031595566No Hit
GGAGACTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGCTG41870.11588075601745491No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATC377700.0142.482732
GCGTTAT382750.0142.231781
TTATCCA392850.0135.454364
GTTATCC394200.0135.12113
TATCCAC406150.0131.429265
CACCTTC425850.0126.7470259
ATCCACC419050.0126.708636
CGTTATG4800.0119.320642
CACGCTG24850.0106.778651
GGGAAGT89900.0106.127011
CCACCTT508250.0105.328048
GTATCAA19100.0104.003249
GAAGTTT92050.0103.175663
AAGTTTC92400.0102.783414
GGAAGTT94000.0100.958182
TTCTGGC98600.0100.586318
TCGTATC27400.098.6933759
TCTGGCG101750.097.899859
CTCGTAT27700.097.1010368
AAGCAGT21150.094.608121
Produced by FastQC (version 0.11.5)