FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4445455
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAAG327350.0143.268461
CGAAGTC325450.0143.080183
TTCGGTC794000.0142.932391
GTCGTAT783900.0142.415655
GTATCGC792150.0142.404318
TCGTATC783450.0142.367896
CGGTCGT783100.0142.32973
TCGGTCG782750.0142.245132
CGTTCTG287400.0142.170681
TATCGCA795600.0142.081599
CGTATCG784900.0142.075587
TCGAAGT328800.0141.644452
GGTCGTA799750.0141.442434
CGAGAAC321300.0141.38739
AAGTCGA320650.0141.152285
AGTCGAG322250.0140.406466
GTCGAGA323350.0139.837557
TTCTACG40750.0136.10052
TCGAGAA334400.0135.823788
ATTCTAC51200.0135.79211
Produced by FastQC (version 0.11.5)