FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3697332
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT2928647.920954893961375No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA1018572.7548783825742453No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT417061.128002570502189No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT365900.9896325242093489No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT293530.7938967882786832No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT238820.6459252239182199No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG227230.6145782959171641No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG182690.4941130523307077No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG146190.39539321867768434No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC140550.38013897588855966No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC134510.3638028719087169No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA128890.3486027221791281No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT102130.2762262085200896No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG95920.2594303135341917No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC83660.22627126803868303No Hit
CCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGC77830.21050314118396724No Hit
GTCCCAGGTCACCATCACCGGCTCCGGGAAGTAGCCCGTGGCCAGGCAGC70670.1911378258701139No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG66060.17866937564708824No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG51000.13793730181655312No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT47280.12787599274287514No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG45520.12311580350371565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44120.11932928933620242No Hit
GGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTGGG42000.11359542502539668No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG41440.1120808193583914No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC41100.11116123734628104No Hit
CTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCG41010.11091781857836948No Hit
TCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAG40240.1088352357862372No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)