FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458983
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTCG891900.0143.47481
CGTCTCG888850.0143.339086
TTCGTCT890500.0143.122334
TCTTCGT889100.0143.111242
CTTCGTC890150.0142.991293
TCGTCTC891950.0142.922185
GTCTCGC893200.0142.703877
ATCGATA83900.0142.229722
TCTCGCT901350.0142.145468
CTCGCTT904550.0141.550529
TCGATAG84750.0140.546593
ATAGATG82650.0140.258156
GATCGAT86250.0138.774751
TAGATGT89600.0134.878837
AGACACT36200.0131.778959
TCTCTAA32850.0131.753281
TAACGCG24700.0130.908149
TCCCACA40300.0129.70383
TAACGTA7050.0128.54152
ACGTTAG20600.0128.102223
Produced by FastQC (version 0.11.5)