FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458983
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34592210.000685172491453No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA391531.131922302017674No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT264940.7659476788408617No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT149070.4309648240537753No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT134130.38777293788376527No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA121340.3507967515307245No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT116950.33810515981142436Clontech SMART CDS Primer II A (100% over 26bp)
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG80220.23191787875222283No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC75760.21902391541097485No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT73490.2124612928135235No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC73220.21168071655743897No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT72280.2089631547769966No Hit
GGGGCTTTCCCTGGTCTCTGCTTATACCAGGCCAACCAACTCCTAACACT57760.16698549833867354No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC56970.16470159003383364No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG55010.15903518461929417No Hit
GGAGGTTAGGGGCTTTCCCTGGTCTCTGCTTATACCAGGCCAACCAACTC54610.15787877535102082No Hit
GTGATGGTGACTCTGTCCCCTACAGATGCAGACAGGGTGGAAGGAGACTG51650.14932134676579792No Hit
GGCCCGGCAAGTGATGGTGACTCTGTCCCCTACAGATGCAGACAGGGTGG45280.13090552916854464No Hit
GCTGAACCTAGATGGGACCCCACTTTCTAAAATAGACGCCACATAGATCA43630.126135340936917No Hit
GCTTATACCAGGCCAACCAACTCCTAACACTCTGACTGGCCCGGCAAGTG43620.12610643070521016No Hit
GGCTTTCCCTGGTCTCTGCTTATACCAGGCCAACCAACTCCTAACACTCT43610.12607752047350335No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG41280.119341436485811No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG40030.11572765752245674No Hit
CCAGGCCAACCAACTCCTAACACTCTGACTGGCCCGGCAAGTGATGGTGA39770.11497599149807905No Hit
GGCCAACCAACTCCTAACACTCTGACTGGCCCGGCAAGTGATGGTGACTC39360.11379067199809886No Hit
GATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGC38520.11136221253472481No Hit
GACGCCACATAGATCAGGAGGTTAGGGGCTTTCCCTGGTCTCTGCTTATA38300.11072618743717445No Hit
GACCCCACTTTCTAAAATAGACGCCACATAGATCAGGAGGTTAGGGGCTT37660.10887593260793707No Hit
CTCTGCTTATACCAGGCCAACCAACTCCTAACACTCTGACTGGCCCGGCA37100.10725695963235438No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC36500.10552234572994432No Hit
GGTCAGTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCGCTGAAC35670.1031227964982771No Hit
GTCCCAGATCCACTGCCGCTGAACCTAGATGGGACCCCACTTTCTAAAAT35320.10211093838853791No Hit
GTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCGCTGAACCTAGA35270.10196638723000374No Hit
CAACCAACTCCTAACACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGT35110.10150382352269438No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)