FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3819941
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA290700.0143.697681
TACAGTG430450.0142.922911
TGAGACG428050.0142.487886
GACGCAC430400.0142.351849
CCAAATC353450.0142.307512
AGTGAGA429950.0142.161714
AGACGCA431050.0142.101678
GAGACGC430100.0141.842447
AATGAAA292850.0141.825762
CGTCATA209100.0141.492727
AGCGTCA209200.0141.459755
TGAAAGG293750.0141.391224
AAAGGAT292000.0140.922746
GCGTCAT210100.0140.819266
AGGATCG296400.0140.322788
GGATCGC296500.0140.301749
ACAGTGA435100.0140.27912
AAGGATC296550.0140.178457
ATGAAAG296550.0140.007323
GTCATAT215700.0139.751318
Produced by FastQC (version 0.11.5)