FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3819941
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38502210.07926562216537No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA526751.3789479994586304No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT195030.5105576237957602No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT176100.46100188458408126No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT175780.4601641753105611No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA139160.364298820322094No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT138420.3623616176270785No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG86190.22563175713970451No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85930.22495111835496936No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG74890.1960501484185227No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT72380.18947936630434867No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT64250.16819631507397628No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC63020.16497637005388305No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC61720.16157317613020725No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG58230.15243690936587764No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT56610.14819600616868167No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC53940.14120636941774756No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC53360.1396880213594922No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG46200.12094427636447787No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG42680.11172947435575575No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC38430.10060364806681568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATC487250.0142.511722
GCGTTAT494100.0141.860261
TTATCCA504300.0136.385274
TATCCAC533400.0128.835955
GTTATCC533750.0128.778643
CACGCTG77200.0128.65961
CACCTTC541550.0127.693589
CCACCTT556850.0123.312728
CGTTATG6150.0120.241012
ATCCACC621150.0110.412076
TCCACCT623700.0109.7993557
GTATCAA17000.0103.635299
TCGTATC95800.0103.152729
CTCGTAT95600.0103.140998
GCTCGTA99300.099.219677
CTGCTCG99700.098.82165
AAGTTTC47600.096.2581944
AGTTTCT49250.094.210945
ACGCTGC107300.092.632912
TGCTCGT106200.092.36246
Produced by FastQC (version 0.11.5)