FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3804636
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1407593.6996706123792134No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT644601.694248805930449No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC637741.676218171725232No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA191100.5022819528596165No Hit
AGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCT167290.4397004076079814No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT135150.35522452082143996No Hit
GCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTC127400.33485463523974435No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG125240.3291773510002008No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT96330.2531911068496434No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT75980.19970372987061047No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42570.11188981022100407No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG40130.10547658172818636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT238200.0132.213471
CGTTATC84400.0130.73742
GTATCAA255050.0124.2177669
GGTATCA260900.0121.2388
AGTGGTA261900.0119.9691545
CAGTGGT262350.0119.293624
AGCAGTG265100.0118.824962
GCAGTGG278550.0113.6845253
ATCCACC97050.0113.243736
GTGGTAT275550.0113.0249566
TATCCAC100050.0110.50175
TGGTATC282200.0109.4124767
CTCGTAT35800.0100.242018
TTATCCA111250.099.311874
GCGTTAT116250.096.913811
TCGTATC37600.094.479129
CTACACG16450.094.313851
CACGCTG38550.094.031471
GTTATCC120050.091.308573
TACACGA17650.087.901582
Produced by FastQC (version 0.11.5)