FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005067828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3734701
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA144890.38795609072854825No Hit
TGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC76470.20475534721521213No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACC67370.18038927346526537No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG64990.1740166080229716No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT63360.16965213547215696No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC61640.1650466797743648No Hit
GTGGGTCTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT60830.16287783145156734No Hit
TTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGC55630.14895436073731205No Hit
CCACCTACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG53310.1427423507263366No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT42220.11304787183766518No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTT41320.11063804036789023No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA40270.10782657031981945No Hit
CGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG39020.10447958216735422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)