FastQCFastQC Report
Wed 8 May 2024
EGAF00005067830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988836
Total Bases149.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG115411.167129837505916No Hit
TTGTAGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGC39160.39602118045864027No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC36340.36750280127341645No Hit
CCACCTACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG26380.2667783130873067No Hit
AAACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC25380.25666541266701454No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA23320.23583283780121275No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC21660.2190454231035278No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA21160.21398897289338173No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTC20450.20680881359497427No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA18100.18304349760728778No Hit
TTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGC17960.18162769154844685No Hit
TTTCCTCTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGA17290.17485204826685113No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT16530.1671662439474291No Hit
CGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG12280.1241864171611875No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT11810.11943335396365018No Hit
AGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA11490.1161972258291567No Hit
TGCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTG11360.11488254877451874No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACTAACCTTTAATAGCGGC10560.10679222843828502No Hit
ATGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTG10490.10608432540886456No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)