FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005067835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005067835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574099
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT639454.062323907200246No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA223881.4222739484619455No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT71850.4564515954841468No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC66730.42392505172800443No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT62940.39984778593976616No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62340.39603608159334325No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT42200.26808987236508No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG33480.2126931025303999No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31530.2003050634045254No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG27380.17394077500843338No Hit
AGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCT25200.16009158254976338No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC24890.1581222019707782No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA24710.15697869066685133No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG24430.1551998953051873No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT22730.14440006632365562No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG21540.1368401860365835No Hit
CCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGC20790.1320755556035548No Hit
GCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTC20280.12883560690909532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATC75850.0140.098192
ATCCACC82950.0127.3221056
TATCCAC85050.0124.093125
GCGTTAT90750.0117.028991
TTATCCA94100.0112.008044
CACGCTG32500.0111.681811
CTCGTAT33450.0107.484598
TCGTATC33350.0107.372189
TGCTCGT34050.0105.164816
GTTATCC102500.0103.42423
GTATCAA10200.0100.91349
GGTATCA10550.098.939728
TCCACCG33600.097.72812145
ACGCTGC37300.096.843612
CACCTTC110300.095.75139
CCACCTT110550.095.469198
GCTCGTA40300.089.5746157
CTGCTCG41250.088.0388645
AAGTTTC23400.087.0492254
CGCTGCT41700.086.594823
Produced by FastQC (version 0.11.5)